Evaluation of Curcumin Effects on Post-Operative Peritoneal Adhesion in Rats

Document Type: Original Article

Authors

1 Department of Surgery, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Pharmaceutical Research Centre, Mashhad University of Medical Science, Mashhad, Iran

3 Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

4 Endoscopic &Minimally Invasive Surgery Research Centre, Ghaem hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s)
The purpose of this study holds, for the first time, an evaluation of the intraperitoneal curcumin lavage on the development of post-operative intra-abdominal adhesions.
Materials and Methods
Thirty male Wistar rats were randomized into five groups. The rats were administered anesthesia and underwent surgery in order to create intra-abdominal adhesions. Before the abdomen was closed, five lavage solutions of normal saline (control group), curcumin 1, 3, and 5% and hydrocortisone 1% were used for 1 min. After five days, the rats underwent laparatomy. Based on a histopathology evaluation and serum levels of hs-CRP, TNFα and Isoprostane, peritoneal adhesion severity were compared in different groups.
Results
The groups that received curcumin 3% and 5% showed a significant decrease in TNFα, hs-CRP and Isoprostane serum concentrations compared to the normal saline group, however, these differences were not significant, between the other groups. The intensity of adhesions in the different groups of curcumin 1, 3 and 5% concentrations and hydrocortisone 1% were compared to the normal saline control group and no significant statistical difference was recorded.
Conclusion
Curcumin was not effective in post-operative peritoneal adhesion; however, further studies on curcumin lavage in higher concentrations are recommended.

Keywords


1. Menzies D, Ellis H. Intestinal obstruction from adhesions how big is the problem? Ann R Coll Surg Engl 1990; 72:60.
2. Gutt CN, Oniu T, Schemmer P, Mehrabi A, Büchler MW. Fewer adhesions induced by laparoscopic surgery? Surg Endosc 2004; 18:898-906.
3. Ray NF, Denton WG, Thamer M, Henderson SC, Perry S. Abdominal adhesiolysis: Inpatient care and expenditures in the United States in 1994. J Am Coll Surg 1998; 186:1-9.
4. Lauder CI, Strickland A, Maddern GJ. Use of a modified chitosan-dextran gel to prevent peritoneal adhesions in a porcine hemicolectomy model. J Surg Res 2011; 176:448-454.
5. Cheong YC, Laird SM, Shelton JB, Ledger WL, Li TC, Cooke ID. The correlation of adhesions and peritoneal fluid cytokine concentrations: A pilot study. Hum Reprod 2002; 17:1039-1045.
6. Liakakos T, Thomakos N, Fine PM, Dervenis C, Young RL. Peritoneal adhesions:Etiology, pathophysiology, and clinical significance. Recent advances in prevention and management. Dig Surg 2001; 18:260.
7. Heydrick SJ, Reed KL, Cohen PA, Aarons CB, Gower AC, Becker JM, et al. Intraperitoneal administration of methylene blue attenuates oxidative stress, increases peritoneal fibrinolysis, and inhibits intraabdominal adhesion formation. J Surg Res 2007; 143:311–319.
8. Zeng Q, Yu Z, You J, Zhang Q. Efficacy and safety of Seprafilm for preventing post-operative abdominal adhesion: Systematic review and meta-analysis. World J Surg 2007; 31:2125-2132.
9. Tang CL, Jayne DG, Seow-Choen F, Ng YY, Eu KW, Mustapha N. A randomized controlled trial of 0.5% ferric hyaluronate gel (Intergel) in the prevention of adhesions following abdominal surgery. Ann Surg 2006; 243:449.
10. Goel A, Kunnumakkara AB, Aggarwal BB. Curcumin as ‘Curecumin’’: From kitchen to clinic. Biochem Pharmacol 2008; 7: 787– 809.
11. Naik SR, Thakare VN, Patil SR. Protective effect of curcumin on experimentally induced inflammation, hepatotoxicity and cardiotoxicity in rats:Evidence of its antioxidant property. Exp Toxicol Pathol 2011; 63:419-431.
12. Whang SH, Astudillo JA, Sporn E, Bachman SL, Miedema BW, Davis W, et al. In search of the best peritoneal adhesion model: comparison of different techniques in a rat model. J Surg Res 2011; 167:245-250.
13. Sharma C, Kaur J, Shishodia S, Aggarwal BB, Ralhan R. Curcumin down regulates smokeless tobacco-induced NF-β activation and COX-2 expression in human oral premalignant and cancer cells. Toxicology 2006; 228:1–15.
14. Varalakshmi Ch, Ali AM, Pardhasaradhi BV, Srivastava RM, Singh S, Khar A. Immunomodulatory effects of curcumin: in-vivo. Int Immunopharmacol 2008; 8:688–700.
15. Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin: A short review. Life Sci 2006; 78:2081–2087.
16. Cekmen M, Ilbey YO, Ozbek E, Simsek A, Somay A, Ersoz C. Curcumin prevents oxidative renal damage induced by acetaminophen in rats. Food Chem Toxicol 2009; 47:1480–1484.
17. Ramirez-Tortosa MC, Ramirez-Tortosa CL, Mesa MD, Granados S, Gil A, Quiles JL. Curcumin ameliorates rabbits's steatohepatitis via respiratory chain, oxidative stress and TNF-α. Free Radic Biol Med 2009; 47:924–931.