Evaluation of Porin Interaction with Adenine Nucleotide Translocase and Cyclophilin-D Proteins after Brain Ischemia and Reperfusion

Document Type: Original Article

Authors

1 Anatomical Sciences Research Center, Kashan University of Medical Sciences, Kashan, Iran

2 Department of Neurology and Pediatrics, the Hospital for Sick Children, Brain and Behavior Programme, University of Toronto, Ontario, Canada

Abstract

Objective (s)
Porin is a mitochondrial outer membrane channel, which usually functions as the pathway for the movement of various substances in and out of the mitochondria and is considered to be a component of the permeability transition (PT) pore complex that plays a role in the PT. We addressed the hypothesis that porin interacts with other mitochondrial proteins after ischemic injury.
Materials and Methods
For this purpose, we used in vivo 4-vessel occlusion model of rat brain and porin purification method by hydroxyapatite column. After SDS gel electrophoresis and silver nitrate staining, Western blotting was done for porin, adenine nucleotide translocase and cyclophilin-D proteins.
Results
Porin was purified from mitochondrial mixture in ischemic brain and control groups. Investigation of interaction of adenine nucleotide transposes (ANT) and cyclophilin-D with porin by Western blotting showed no proteins co-purified with porin from injured tissues.
Conclusion
The present study implies that there may not be interaction between porin, and ANT or cyclophilin-D, and if there is any, it is not maintained during the purification procedure.

Keywords


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