The Effect of Sun Radiation on the Course of Cutaneous Leishmaniasis in BALB/c Mice

Document Type: Original Article

Authors

1 Department of Dermatology, Emamreza Hospital, Mashhad, Iran

2 Department of Dermatology, Gaem Hospital, Mashhad, Iran

3 Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

4 Mashhad University of Medical Sciences, Mashhad, Iran

5 Research Center for Skin Diseases and Cutaneous Leishmaniasis, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s)
Studies have described immunomedulatory effects of sun exposure and ultraviolet radiation on infectious and neoplastic diseases. Here the effect of exposure to low potency radiation of sun on the course of leishmaniasis in mice was studied.
Materials and Methods
Fifteen BALB/c mice were exposed to suberythemogenic doses of sun (mean 180 mJ/cm2/day of UVB) 2 months before and 4 months after Leishmania major inoculation to food pad. Control group was kept in the sun protected environment. From 2nd to 17th week after inoculation, size of the lesion was recorded in each group weekly and at last week the parasite burden in spleen was detected. Results were compared between two groups.
Results
Seven mice from case group and 9 mice from control group survived up to last week. The mean lesion size was 0.90±0.59 cm in exposed and 4.01±3.59 cm in unexposed mice (P= 0.037). Parasite burden in spleen of case and control groups were 5.5±4.61 and 106.94±279.76 respectively (P= 0.006).
Conclusion
Chronic exposure of BALB/c mice to suberythemogenic doses of sun suppressed skin lesion and decreased the extension of L. major to spleen.

Keywords


1. Ardehali SD, Rezaee HR, Nadim AH. Leishmaniasis and Leishmania parasite. Tehran-Iran: Tehran university publication center; 1985.

2. Kemp M, Hey AS, Kurtzhals JA, Christensen CB, Gaafar A, Mustafa MD, et al. Dichotomi of the T cell responseto Leishmania antigens. I.Th1-like response to Leishmania major promastigote antigens in individuals recovered from cutaneousleishmaniasis. Clin Exp Immunol 1994; 96:410–415.

3. Lopez, FV, Hay RJ. Parasitic worms and protozoa. In: Burns T, Breathnatch S, Cox N, Griffiths C. Rook’s Text book of Dermatology .8th ed.Oxford: Blackwell Science; 2010.p. 33-38.

4. Ghosn S, Kurban A. Leishmaniasis and other protozoan in infections. In: Wolff K, Gold Smith L, Katz S, Gilchrest BA, Paller AS, Leffell DJ. Fitzpatrick's Dermatology in General Medicine. 7th ed. New York: Mc Graw Hill; 2008.p. 2001-2010.

5. Hawk JLM, Young AR, Ferguson J. Cutaneous Photobiology. In: Burns T, Breathnatch S, Cox N, Griffiths C. Rook’s Text book of Dermatology. 8th ed.Oxford: Mc Graw Hill; 2010.p.1-29.

6. Musa AM, Khalil EA, Raheem MA, Zijlstra EE, Ibrahim ME, Elhassan IM, et al. The natural history of Sudanese post-kala-azar dermal leishmaniasis: clinical, immunological and prognostic features. Ann Trop Med Parasitol 2002; 96:765–772.

7. Ismail A, Khalil EA, Musa AM, El Hassan IM, Ibrahim ME, Theander TG, et al. The pathogenesis of post kalaazar dermal leishmaniasis from the field to the molecule: does ultraviolet light (UVB) radiation play a role? Med Hypotheses 2006; 66:993–999.

8. Khaskhely NM, Maruno M, Uezato H, Takamiyagi A, Ramzi ST, Al-Kasem KM, et al. Low-dose UVB contributes to host resistance against Leishmania amazonensis infection in mice through induction of gamma interferon and tumor necrosis factor alpha cytokines. Clin Diagn Lab Immunol 2002; 9:677-686.

9. Jaafari MR, Bavarsad N, Fazly Bazzaz A, Samiei A, Soroush D, Ghorbani S, et al. The effect of topical liposomes containing paromomycin sulfate (PM) in the course of Leishmania major infection in susceptible BALB/c mice. Antimicrob Agents Chemother 2009; 53:2259-2265.

10. Jaafari MR, Ghafarian A, Farrokh-Gisour A, Samiei A, Kheiri MT, Mahboudi F. Immune response and protection assay of recombinant major surface glycoprotein of Leishmania (rgp63) reconstituted with liposomes in BALB/c mice. Vaccine 2006; 24: 708-5717.

11. Brown EL, Rivas JM, Ullrich SE, Young CR,Norris SJ, Kripke ML. Modulation of immunity to Borrelia burgdorferi by ultraviolet irradiation: differential effect on Th1 and Th2 immune responses. Eur J Immunol 1995; 25:3017-3022.

12. Denkins Y, Fidler IJ, Kripke ML. Exposure of mice to UV-B radiation suppresses delayed hypersensitivity to Candida albicans. Photochem Photobiol 1989; 49:615-619.

13. Howie S, Norval M, Maingay J. Exposure to low-dose ultraviolet radiation suppresses delayed- Type hypersensitivity to herpes simplex virus in mice. J Invest Dermatol 1986; 86: 125-128.

14. Jeevan A, Kripke ML. Effect of a single exposure to ultraviolet radiation on Mycobacterium bovis bacillus Calmette- Guerin infection in mice. J Immunol 1989; 143:2837-2843.

15. Jeevan A, Gilliam K, Heard H, Kripke ML. Effects of ultraviolet radiation on the pathogenestis of Mycobacterium lepraemurin in mice. Exp Dermatol 1992; 1:152-160.

16. Noonan FP, Alewis FA. UVB- induced immune suppression and infection with Schistosoma mansoni. Photochem Photobiol 1995; 61:99-105.

17. Garssen J, Vandebriel RJ, De Gruijl FR, Wolvers DA, Van Dijk M, Fluitman A.UVB exposure- induced systemic modulation of Th1-and Th2-mediated immune responses. Immunology 1999; 97:506-514.

18. Reiner SL, Locksley RM. The regulation of immunity to Leishrmania major. Annu Rev Immunol 1995; 13:151-177.

19. Abbas AK, Murphy KM, Sher A. Functional diversity of helper T lymphocytes. Nature 1996; 383:787-793.

20. Louis J, Himmelrich H, Parra-Lopez C, Tacchini-Cottier F, Launois P.Regulation of protective immunity against Leishmania major in mice. Curr Opin Immunol 1998; 10:459-464.

21. Schade N, Esser C, Krutmann J. Ultraviolet B radiation-induced immunosuppression: molecular mechanisms and cellular alterations. Photochem Photobiol Sci 2005; 4:699-708.

22. Schwarz T. Mechanisms of UV-induced immunosuppression. Keio J Med 2005; 54:165–171.

23. Sleijffers A, Garssen J, Vos JG, Loveren H. Ultraviolet light and resistance to infectious diseases. J Immunotoxicol 2004; 1:3-14.

24. Narbutt J, Lesiak A, Sysa-Jedrzejowska A, Wozniacka A, Cierniewska-Cieslak A, Boncela J,et al. Repeated low-dose ultraviolet (UV) B exposures of humans induce limited photoprotection against the immune effects of erythemal UVB radiation. Br J Dermatol 2007; 156: 539-547.

25. McLoone P, Norval M. Adaptation to the UV-induced suppression of phagocytic activity in murine peritoneal macrophages following chronic exposure to solar simulated radiation. Photochem Photobiol Sci 2005; 4:792-797.

26. Giannini MS. Suppression of pathogenesis in cutaneous leishmaniasis by UV irradiation. Infect Immun 1986; 51:838-843.

27. Khaskhely NM, Maruno M, Takamiyagi A, Uezato H, Kasem KM, Hosokawa A, et al. Pre-exposure with lowdose UVA suppresses lesion development and enhances Th1 response in BALB/c mice infected with Leishmania amazonensis. J Dermatol Sci 2001; 26:217-232.

28. Kricker A, Armstrong BK, English DR, Heenan PJ. A dose- response curve for sun exposure and basal cell carcinoma. Int J cancer 1995; 60:482-488.

29. Kricker A, Armstrong BK, English DR, Heenan PJ. Dose intermittent sun exposure and basal cell carcinoma? A case-Control study in Western Australia. Int J cancer 1995; 60:489-494.

30. Lee JAH. Melanoma and exposure to sunlight. Epidermol Rev 1982; 4:110-136.

31. Osterlind A, Tucker MA, Stone BJ, Jensen OM. The Danish case-control study of cutaneous malignant melanoma. II. Importance of UV-light exposure. Int J cancer 1988; 42:319-324.