Polymorphisms within Exon 9, But Not Intron 8, of the Vitamin D Receptor Gene Are Associated with Asthma

Document Type: Original Article

Authors

1 Department of Microbiology, Hematology and Immunology, Faculty of Medicine, Rafsanjan University of Medical Science, Rafsanjan, Iran

2 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan-Iran.

3 Department of Infectious Disease, Faculty of Medicine, Rafsanjan University of Medical Science, Rafsanjan, Iran

4 Molecular Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan-Iran

5 Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

6 Ali-Ebne Abitaleb Hospital, Rafsanjan University of Medical Science, Rafsanjan, Iran

7 School of Biomolecular and Physical Science, Eskitis Institute for Cell and Molecular Therapies, Griffith University Nathan, Queensland-Australia

Abstract

Objective(s)
Deregulation of the immune system through allied factors and cytokine responses are thought to be important contributors to the pathogenesis of asthma. Vitamin D3 and its nuclear receptor appear to be factors that maybe involved in regulating immune responses during the progression of asthma. The aim of this study was to investigate the association between polymorphisms in intron 8 and exon 9 of the vitamin D receptor (VDR) and this disease.
Materials and Methods
This study was performed on 100 asthmatic patients and 100 healthy controls. PCR-RFLP was performed to examine polymorphisms in intron 8 and exon 9 of VDR gene.
Results
Our results showed a statistically significant difference in the Taq-1 evaluated genotypes of exon 9 of the VDR gene when comparing healthy patients to asthmatic patients.
Conclusion
Based on our results, it can be concluded that VDR and its functional polymorphisms may play an important role in the pathogenesis of asthma.

Keywords


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