Immunolocalization of Galectin-3 in Mouse Testicular Tissue

Document Type: Original Article


Cell & Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran


Galectin-3 (Gal-3) is a member of the B-galactoside-binding lectins which is expressed in a variety of tissues and plays a role in diverse biological events, such as embryogenesis, adhesion, cellular proliferation, and apoptosis. In this study, the presence and distribution of galectin-3 (Gal-3) in the mouse testicular tissue was investigated.
Materials and Methods
Eight adult NMRI mice were used in this study. Animals were sacrificed by decapitation under ether anesthesia. The testes were excised, fixed in 10% buffered formalin and embedded in paraffin for immunohistochemical (IHC) studies. H-score, a semi-quantitative method, was used for scaling the immunostaining.
Positive immunoreactivity to Gal-3 was detected in the connective tissues of the interstitium, Leydig cells and presumably peritubular myoid cells. Seminiferous tubules showed immuno-positive reaction in a stage dependent manner. Stages I-III and IX-XII of spermatogenic cycle showed mild immunostaining, while moderate immunostaining was observed at stages IV-VI. Highest immunoreactivity was observed at stages VII-VIII. H-score assessment showed a significant increase in stages of VII-VIII in comparison to other stages (P< 0.05).
Expression of Gal-3 in interstitial tissue and seminiferous tubules indicated that this protein probably has multifunctional roles in the mouse testicular tissue.


1. Krzeslak A, Lipinska. A Galectin-3 as a multifunctional protein. Cell Mol Biol Lett 2004; 9: 305-328.

2. Xu XC, el-Naggar AK, Lotan R. Differential expression of galectin-1 and galectin-3 in thyroid tumors. Potential diagnostic implications. Am J Pathol 1995; 147:815-822.

3. Berberat PO, Friess H, Wang L, Zhu Z, Bley T, Frigeri L, et al. Comparative analysis of galectins in primary tumors and tumor metastasis in human pancreatic cancer. Histochem Cytochem 2001; 49:539-549.

4. Lotz MM, Andrews CW, Korzelius CA, Lee EC, Steele GD, Clarke A, et al. Decreased expression of Mac-2 (carbohydrate binding protein 35) and loss of its nuclear localization are associated with the neoplastic progression of colon carcinoma. Proc Natl Acad Sci USA 1993; 90:3466-3470.

5. Song YK, Billiar TR, Lee YJ. Role of galectin-3 in breast cancer metastasis: involvement of nitric oxide. Am J Pathol 2002; 160:1069-1075.

6. Barondes SH, Cooper DN, Gitt MA, Leffler H. Galectins: Structure and function of a large family of animal lectins. J Chem Biol 1994; 269:20807-20810.

7. Davidson PJ, Davis MJ, Patterson RJ, Ripoche MA, Poirier F, Wang JL. Shuttling of galectin-3 between the nucleus and cytoplasm. Glycobiology 2002; 12:329-327.

8. Yu F, Finley RL, Raz A, Kim HR. Galectin-3 translocates to the perinuclear membranes and inhibits cytochrome c release from the mitochondria. A role for synexin in galectin-3 translocation. Biol Chem 2002; 277:15819-15827. 

9. Dagher SF, Wang JL, Patterson RJ. Identification of galectin-3 as a factor in pre-mRNA splicing. Proc Natl Acad Sci USA 1995; 92:1213-1217.

10. Akahani S, Nangia-Makker P, Inohara H, Kim HR, Raz A. Galectin-3: A novel antiapoptotic molecule with a functional BH1 (NWGR) domain of Bcl-2 family. Cancer Res 1997; 57:5272-5276.

11. Kim HR, Lin HM, Biliran H, Raz A. Cell cycle arrest and inhibition of anoikis by galectin-3 in human breast epithelial cells. Cancer Res 1999; 59:4148-4154.

12. Yang RY, Hsu DK, Liu FT. Expression of galectin-3 modulates T-cell growth and apoptosis. Proc Natl Acad Sci USA 1996; 93:6737-6742.

13. Nio J, Takahashi-Iwanaga H, Morimatsu M, Kon Y, Iwanaga T. Immunohistochemical and in situ hybridization analysis of galectin-3, a beta-galactoside binding lectin, in the urinary system of adult mice. Histochem Cell Biol 2006; 126:45-156

14. Kaltner H, Seyrek K, Heck A, Sinowatz F, Gabius HJ. Galectin-1 and galectin-3 in fetal development of bovine respiratory and digestive tracts. Cell Tissue Res 2002; 307:35-46.

15. Kim H, Kang T, Joo H, Shin T. Immunohistochemical localization of galectin-3 in boar testis and epididymis. Acta Histochem 2006; 108:481-485.

16. Ha TY, Ahn MJ, Lee YD, Yang JH, Kim HS, Shin TK. Histochemical detection of glycoconjugates in the male reproductive system of the horse. Vet Sci 2003; 4:21-28.

17. Khorsandi LS , Hashemitabar M, Orazizadeh M, Albughobeish N. Dexamethasone effects on fas ligand expression in mouse testicular germ cells. Pak J Biol Sci 2008; 11:2231-2236.

18. Mahmoud H, Mahmoud O, Layasadat K, Naeim A. Dexamethasone effects on Bax expression in the mouse testicular germ cells. Folia Histochemt Cytobiol 2009; 47:237-241.

19. Russell LD, Ettlin RA, Sinha AP, Clegg ED. Histological and histopathological evaluation of the testis. 1st ed. Florida: Cache River; 1990.

20. Weltzien F, Taranger LG, Karlsen R, Norberg B. Spermatogenesis and related plasma androgen levels in Atlantic halibut (Hippoglossus hippoglossus L.). Comp Biochem Physiol 2002; 132:567-575.

21. Deschildre C, Ji JW, Chater S, Dacheux F, Selva J, Albert M, et al. Expression of galectin-3 and its regulation in the testes. Int J Androl 2007; 30:28-40.

22. Devouassoux-Shisheboran M, Deschildre C, Mauduit C, Berger G, Mejean-Lebreton F, Bouvier R, et al. Expression of galectin-3 in gonads and gonadal sex cord stromal and germ cell tumors. Oncol Rep 2006; 16:335-340.

23. Kim H, Kang TY, Joo HG, Shin T. Immunohistochemical localization of galectin-3 in boar testis and epididymis. Acta Histochem 2006; 108:481-485.

24. Kim H, Ahn M, Moon C, Kim S, Jee Y, Joo H, Shin T. Immunohistochemical study of galectin-3 in mature and immature bull testis and epididymis. J Vet Sci 2008; 9:339-344.

25. Bremner WJ, Millar MR, Sharpe RM, Saunders PT. Immunohistochemical localization of androgen receptors in the rat testis: evidence for stage-dependent expression and regulation by androgens. Endocrinology 1994; 135:1227-1234.

26. Orazizadeh M, Hashemitabar M, Khorsandi LS. Protective effect of minocycline on dexamethasone induced testicular germ cell apoptosis in mice. Eur Rev Med Pharmacol Sci 2009; 13:1-5.

27. Orazizadeh M, Khorsandi LS, Hashemitabar M. Toxic effect of Dexamethasone on mouse testiculsr germ cell. Andrologia 2010; 42:247-253.

28. Stillman BN, Hsu DK, Pang M, Brewer CF, Johnson P, Liu FT, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. Immunology 2006; 176:778-789.

29. Woo HJ, Shaw LW, Messier JM, Mercurio AM. The major non-integrin laminin binding protein of macrophages is identical to carbohydrate binding protein 35 (Mac-2). Biol Chem 1990; 265: 7097-7099.

30. Tapanainen JS, Tilly JL, Vihko KK, Hsueh AJ. Hormonal control of apoptosis cell death in the testis: Gonadotropin and androgens as testicular cell survival factors. Mol Endocrinol 1993; 7:643-650.

31. Boekelheide K, Fleming SL, Johnson KJ, Patel SR, Schoenfeld HA. Role of Sertoli cells in injury-associated testicular germ cell apoptosis. Proc Soc Exp Biol Med 2000; 225:105-115.

32. De Franga LR, Ghosh S, Ye SJ, Russell LD. Surface and surface to volume relationships of the Sertoli cells during the cycle of the seminiferous epithelium in the rat. Boil Reprod 1993; 49:1215-1228.