Antiviral Activity of Obtained Extracts from Different Parts of Cupressus sempervirens against Herpes Simplex Virus Type 1

Document Type: Original Article


1 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Pharmacology and Pharmacological Research Centre of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

4 Department of Microbiology, Quaem Medical Center, Mashhad University of Medical Sciences, Mashhad, Iran


The aim of this study was to search for new antiviral agents from herbal medicines. Ethanol extracts of C. semipervirens, C. semipervirens var. horizontalis and C. semipervirens cv. Cereiformis were used in experiments to test their influence on herpes viruses (HSV-1).
Materials and Methods
HeLa cells monolayers were infected with herpes viruses (HSV-1). Antiviral activity of the plant extracts assessed using Hematoxylin & Eosin method and observed under a light microscope. All tests were compared with a positive control, acyclovir.
Results showed that all three plants have antiviral activity against HSV-1 virus. The most active extract was the obtained extract from C. semipervirens. Among the different parts of this medicinal plant tested, the fruit’s extract appeared to possess the strongest anti- HSV activity.
In conclusion, of the extracts tested in this survey all showed significant antiviral potency.


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