Evaluation of Antibacterial Activities of Some Medicinal Plants from North-West Iran

Document Type: Original Article

Authors

1 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz

2 Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

4 Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Objective(s)                                                                                                                                                                               Aim of the present study was to screen the antibacterial activities of some medicinal plants extracts traditionally used in Azarbaijan area (Iran).
Materials and Methods
Thirty-six extracts obtained from different parts of ten plants including Tanacetum balsamita L. (Copmositae), Muscari caucasicum Baker (Hyacinthaceae), Equisetum arvense L. (Equisetaceae), Achillea millefollum L. (Copmositae), Stachys fruticulosa M. Bieb. (Labiatae), Stachys schtschegleevii Sons. ex, Grossh. (Labiatae), Salvia sahendica Boiss & Buhse (Labiatae), Phlomis caucasica Rech. f. (Labiatae), Etchium italicum L. (Boraginaceae) and Thalictrum minus L. (Ranunculaceae) from north-west Iran with traditional medicinal use were examined for their antibacterial activities against some Gram-negative strains such as Escherichia coli, Pseudomonas aeruginosa, Salmonella paratyphi and Serratia marcescens, also, Gram-positive strains of Staphylococcus aureus, Micrococcus luteus, Staph. epidermidis, Streptococcus pneumoniae and Bacillus cereus. The filter paper disc diffusion method as well as broth serial dilution technique were applied to screen the antibacterial efficacy of the extracts and determination of minimum inhibitory values.
Results                                                                                                                                                                              Results indicated that the majority of tested plant extracts had antibacterial activity at least against one of the selected bacteria, with the exception of Muscari caucasicum. Methanol extract of the aerial part of Thalictrum minus L. (Ranunculaceae) showed the most potent antibacterial activity against Staph. aureus with MIC value of 0.3125 mg/ml.
Conclusion
The results of this study show that most of the studied plants are potentially a good source of antimicrobial agents and support the traditional applications of some of the tested plants.
 

Keywords


1. Saad B, AzaizehH, SaidO. Tradition and perspectives of arab herbal medicine: A Review. Evid Based Complement Alternat Med 2005; 2:475-479.

2. Cooper EL. Drug discovery, CAM and natural products. Evid Based Complement Alternat Med. 2004; 1:215-217.

3. Kalhor R. Contribution of Persian physicians to the development of Islamic medicine. Med Arh 1997; 51:9-12.

 

 4. Zakaria M. Isolation and characterization of active compounds from medicinal plants. Asia Pacific J Pharmacol 1991; 6:15-20.

5. WHO. Traditional Medicine Strategy 2002-2005. WHO Publications. P. 1-6.

6. Zaidan  MRS, Noor  Rain A, Badrul  AR, Adlin  A, Norazah  A, Zakiah I. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed 2005;22:165-170.

7. Rojas J, Ochoa J, Ocampo SA, Muñoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complement Altern Med 2006; 6:2.

8. Shahidi B. Evaluation of antibacterial properties of some medicinal plants used in Iran. J Ethnopharmacol 2004; 94: 301-305.

9. Piddock KJV, Wise R. Mechanism of resistance to quinolones and clinical perspective. J Antimicrob Chemother 1989; 23:475-483.

10. Herrera RM, Perez M, Martin-Herrera DA, Lopez-Garcia R, Rabanal RM. Antimicrobial activity of extracts from plants endemic to the Canary Islands. Phytother Res 1996; 10:364-366.

11. Ho KY, Tsai CC, Huang HS, Chen CP, Lin TC, Lin CC. Antimicrobial activity of tannin components from Vaccinium vitis-idaeaL. J Pharm Pharmacol 2001; 53:187-191.

12. Kelmanson JE, Jager AK, Van Staden J.Zulu medicinal plants with antibacterial activity. J Ethnopharmacol 2000; 69:241-246.

13. Mansouri S, Foroumadi A, Ghanei T, Gholamhosseinian Najar A. Antibacterial activity of the crude extracts and fractionated constituents of Myrtus communis. Pharm Biol 2001; 39:399-401.

14. National Committee for Clinical Laboratory Standards. (1996): Performance Standards for Antimicrobial Disk Susceptibility Tests- Sixth Edition: Approved Standard M2-A6. NCCLS, Wayne, PA.

15. Russell AD, Hugo WB, Ayliffe GAJ. Principles and Practice of Disinfection, Preservation and Sterilization. London: Blackwell Scientific Publication ;1982.p.8-106.

16. Topley and Wilson's. Microbiology and microbial infections. 9th ed. 1988.p. 231-256.

17. Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-Candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005; 97:305-311.

18. Skaltsa HD, Demetzos C, Lazari D, Sokovic M. Essential oil analysis and antimicrobial activity of eight stachys species from Greece. Phytochemistry 2003; 64:743-752.

19. Skaltsa HD, Lazari DM, Chinou IB, Loukis AE. Composition and antibacterial activity of the essential oils of Stachys candida and S. chrysantha from southern Greece.Planta Med2001; 65:255-256.

20. Khanavi M, Sharifzadeh M, Hadjiakhoondi A, Shafiee A. Phytochemical investigation and anti-inflammatory activity of aerial parts of Stachys byzanthina C. Koch.  J Ethnopharmacol 2005; 97:463-468.

21. Tortora GJ, Funke BR, Case CL. Microbiology: An Introduction. Benjamin Cummings. San Francisco: 2001; 88.

22. Yao J, Moellering R. Antibacterial agents. In: Murray P, Baron E, Pfaller M, Tenover F, Yolken R. Manual of Clinical Microbiology.Washington DC: ASM Press; 1995.p. 1281-1290.

23. Giamarellos-Bourboulis EJ, Grecka P, Dionyssiou-Asteriou A, Giamarellou H. In vitro interactions of gamma-linolenic acid and arachidonic acid with ceftazoline on multiresistant Pseudomonas aeruginosa. Lipids 1999; 34: 151-152.