Effect of Portulaca oleraceae L. extracts on the Morphine Dependence in Mice

Document Type: Original Article


1 Department of Pharmacodynamy and Toxicology, School of Pharmacy and Medical Research Toxicology Center, Mashhad University of Medical Sciences, Mashhad, Iran

2 School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Pharmacist


This study was initiated to investigate the effect of Portulaca oleraceae on morphine dependence in mice.
Materials and Methods
Dependence was induced using the subcutaneous injections of morphine for 3 days. On the day 4, morphine
injected 2 h prior to intraperitoneal injection of naloxone. The plant extracts ethanolic or aqueous
administered 0.5 h before the final dose of morphine. The number of jumping during the 30 min period after naloxone injection was considered as a measure of withdrawal syndrome.
Both extracts reduced the jumping episodes dose-dependently. The maximum effect was observed at doses
of 0.28 g/kg and 1.4 g/kg for the aqueous and ethanolic extracts, respectively. Clonidine and extracts
decreased the total activity in locomotion test.
These findings indicated that Portulacea oleraceae extracts can decrease morphine dependence in mice.


1. Brickel C. Encyclopedia of gardening. London: Dorling kindersly; 1992. 337.

2. Zargari A. Medicinal plants. Tehran: University Press; 1987. 218-220.

3. Mirheidar H. Encyclopedia of plants. Tehran: Islamic Culture Press; 1995. 72-76.

4. Zannavi MA. Avicenna Canon. Beirut: Dar al-kotob al Iimyah; 1999. 404-406.

5. Parry O, Marks JA, Okwuasaba F. The skeletal muscle relaxant action of Portulaca oleraceae: role of

potassium ions. J Ethnopharmacol 1993; 40: 187-194.

6. Radhakirshnan R, Zakaria MNM, Islam MW. Neuropharmacological actions of Portulaca oleracea L. v sativa (Hawk). J Ethnopharmacol 2001; 76: 171-176.

7. Chan K, Islam MW, Kamil M. The analgesic and anti-inflammatory effects of Portulaca pleraceae L. subsp. sativa (haw) Celak. J Ethnopharmacol 2000; 73: 445-451.

8. Karimi GH, Hosseinzadeh H, Ettehad N. Evaluation of the gastric antiulcerogenic effects of Portulaca oleraceae extracts in mice. Phytother Res 2004; 18: 484-487.

9. Evans WC. Trease and Evans Pharmacognosy. London: Tindall Press; 1996. 161-167.

10. Hosseinzadeh H, Lary P. Effect of Salvia leriifolia leaf extract on morphine dependence in mice. Phytother Res 2000; 14: 384-387.

11. Pardon M, Joubert C, Cohen-Salmon C. Age-dependent effects of chronic ultramild stress procedure on openfield behavior in female mice. Physiol Behav 2000; 70: 7-13.

12. Capasso A, Picacente S, Pizza C, Sorrentino L. Flavonoids reduce morphine withdrawal. J Pharmacol 1999; 50: 561-564.

13. Suzaki T. Tsuda M, Narita M, Funada M, Misawa M. Diazapam pretreatment suppress morphine withdrawal signs in the mouse. Life Sci 1996; 58: 349-357.

14. Yue ME, Jiang TF, Shi YP. Simultaneous determination of noradrenaline and dopamine in Portulaca oleraceae by capillary zone electrophoreses. J Sep Sci 2005; 360-364.

15. Zarrindast MR, Habibi M, Borzabadi S, Rostamian P. The effect of dopamine receptor agent on naloxone induced jumping in morphine dependent mice. Eur J Pharmacol 2002; 457: 287-293.