Effects of gamma oryzanol on factors of oxidative stress and sepsis-induced lung injury in experimental animal model

Authors

1 Iranian Evidence Based Medicine Center of Excellence, Tabriz University of Medical Sciences, Tabriz, Iran

2 Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

3 Drug Applied Research Center, Pharmaceutics Department, Tabriz University of Medical Sciences, Tabriz, Iran

4 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran

5 Drug Applied Research Center, Clinical Pharmacy (Pharmacotherapy) Department, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Objective (s): There is corroborating evidence to substantiate redox imbalance and oxidative stress in sepsis that finally leads to organ damage or even death. Gamma oryzanol (GO) is one of the major bioactive components in rice bran has been considered to function as an antioxidant. The present study was carried out to evaluate the antioxidant activity of gamma oryzanol in vitro and its efficacy in sepsis.
Materials and Methods: To induce sepsis, cecal ligation and puncture (CLP) method was performed on the rats. A study group of forty male Wistar rats were divided into the following groups: sham group; CLP group; 50 mg/kg GO- treated CLP group and 100 mg/kg GO- treated CLP group. GO was administered with an oral gavage 2 hr prior to inducing sepsis. Tissue and blood samples were collected 12 hr after CLP to prepare tissue sections for histopathological study and assay the oxidative stress biomarkers including: SOD (Superoxide Dismutase), TAC (total antioxidant capacity), MDA (Malondialdehyde), MPO (Myeloperoxidase) and PAI-1 (Plasminogen Activator Inhibitor-1). Data are given as mean ± SD. The ANOVA with Tukey post hoc test was used to determine the differences between groups and P Results: TAC level increased in GO- treated CLP groups (P<0.05). Inflammation score of lung tissue and MPO activity were significantly lower in GO treated CLP group (P<0.05).
Conclusion:It seems that GO has a protective effect on lung inflammation and improves the body redox capacity during sepsis.

Keywords


1. Nguyen HB, Rivers EP, Abrahamian FM, Moran GJ, Abraham E, Trzeciak S, et al. Severe sepsis and septic shock: review of the literature and emergency department management guidelines. Ann Emerg Med 2006; 48:54-e51.

2. Chabot F, Mitchell J, Gutteridge J, Evans T. Reactive oxygen species in acute lung injury. Eur Respir J 1998; 11:745-757.

3. Gutteridge JMC, Mitchell J. Redox imbalance in the critically ill. Br Medical Bull 1999; 55:49-75.

4.Bian K, Murad F. Diversity of endotoxin-induced nitrotyrosine formation in macrophage-endothelium-rich organs. Free Radic Biol Med 2001; 31:421-429.

5. Ritter C, Andrades ME, Reinke A, Menna-Barreto S, Moreira JCF, Dal-Pizzol F. Treatment with N-acetylcysteine plus deferoxamine protects rats against oxidative stress and improves survival in sepsis. Crit Care Med 2004; 32:342-349.

6.Zolali E, Hamishehkar H, Maleki-Dizaji N, Zolbanin NM, Ghavimi H, Kouhsoltani M, et al. Selenium effect on oxidative stress factors in septic rats. Adv Pharm Bull 2014; 4:289-293.

7.Toklu HZ, Tunali Akbay T, Velioglu-Ogunc A, Ercan F, Gedik N, Keyer-Uysal M, et al. Silymarin, the antioxidant component of silybum marianum, prevents sepsis-induced acute lung and brain injury. J Surg Res 2008; 145:214-222.

8. Xiao X, Yang M, Sun D, Sun S. Curcumin protects against sepsis-induced acute lung injury in rats. J Surg Res 2012; 176:e31-e39.

9. Ohara K, Kiyotani Y, Uchida A, Nagasaka R, Maehara H, Kanemoto SH, et al. Oral administration of γ-aminobutyric acid and γ-oryzanol prevents stress-induced hypoadiponectinemia. Phytomed 2011; 18:655-660.

10. Lakkakula NR, Lima M, Walker T. Rice bran stabilization and rice bran oil extraction using ohmic heating. Bioresour Technol 2004; 92:157-161.

11. Xu Z, Godber JS. Purification and identification of components of γ-oryzanol in rice bran oil. J Agr Food Chem 1999; 47:2724-2728.

12. Juliano C, Cossu M, Alamanni MC, Piu L. Antioxidant activity of gamma-oryzanol: mechanism of action and its effect on oxidative stability of pharmaceutical oils. Int J Pharm 2005; 299:146-154.

13. Islam M, Murata T, Fujisawa M, Nagasaka R, Ushio H, Bari A, et al. Anti‐inflammatory effects of phytosteryl ferulates in colitis induced by dextran sulphate sodium in mice. Br J Pharmacol 2008; 154:812-824.

14. Wilson TA, Nicolosi RJ, Woolfrey B, Kritchevsky D. Rice bran oil and oryzanol reduce plasma lipid and lipoprotein cholesterol concentrations and aortic cholesterol ester accumulation to a greater extent than ferulic acid in hypercholesterolemic hamsters. J Nutr Biochem 2007; 18:105-112.

15. Mäkynen K, Chitchumroonchokchai C, Adisa-kwattana S, Failla M, Ariyapitipun T. Effect of gamma-oryzanol on the bioaccessibility and synthesis of cholesterol. Eur Rev Med Pharmacol Sci 2012; 16:49-56.

16. Kim JS, Lee J-S, Chang P-S, Lee HG. Optimization, in vitro release and bioavailability of γ-oryzanol-loaded calcium pectinate microparticles reinforced with chitosan. New Biotechnol 2010; 27:368-373.

17. Ghatak SB, Panchal SJ. Investigation of the immunomodulatory potential of oryzanol isolated from crude rice bran oil in experimental animal models. Phytother Res 2012; 26:1701-1708.

18. Reagan-Shaw S, Nihal M, Ahmad N. Dose translation from animal to human studies revisited. FASEB J 2008; 22:659-661.

19. Rittirsch D, Huber-Lang MS, Flierl MA, Ward PA. Immunodesign of experimental sepsis by cecal ligation and puncture. Nat Protoc 2008; 4:31-36.

20. Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. J Invest Dermatol 1982; 78:206-209.

21. Olgen S, Coban T. Antioxidant evaluations of novel N-H and N-substituted indole esters. Biol Pharm Bull 2003; 26:736-738.

22. Atli M, Erikoglu M, Kaynak A, Esen HH, Kurban S. The effects of selenium and vitamin E on lung tissue in rats with sepsis. Clin Investig Med 2012; 35:E48-E54.

23. Hiramitsu T, Armstrong D. Preventive effect of antioxidants on lipid peroxidation in the retina. Ophthalmic Res 1991; 23:196-203.

24. Chotimarkorn C, Ushio H. The effect of trans-ferulic acid and gamma-oryzanol on ethanol-induced liver injury in C57BL mouse. Phytomed 2008; 15:951-958.

25. Babayigit H, Kucuk C, Sozuer E, Yazici C, Kose K, Akgun H. Protective effect of β-glucan on lung injury
after cecal ligation and puncture in rats. Intensive Care Med 2005; 31:865-870.

26. Guo R-F, Ward PA. Role of oxidants in lung injury during sepsis. Antioxid Redox Signal 2007; 9:1991-2002.

27. Chow C-W, Herrera Abreu MT, Suzuki T, Downey GP. Oxidative stress and acute lung injury. Am J Respir Cell Mol Biol 2003; 29:427-431.

28. Mimuro J. Type 1 plasminogen activator inhibitor: its role in biological reactions. Rinsho Ketsueki, Jpn J Clin Hematol 1991; 32:487-489.

29. Madoiwa S, Nunomiya S, Ono T, Shintani Y, Ohmori T, Mimuro J, et al. Plasminogen activator inhibitor 1 promotes a poor prognosis in sepsis-induced disseminated intravascular coagulation. Int J Hematol 2006; 84:398-405.

30. Robbie L, Dummer S, Booth N, Adey G, Bennett B. Plasminogen activator inhibitor 2 and urokinase‐type plasminogen activator in plasma and leucocytes in patients with severe sepsis. Br J Haematol 2000; 109:342-348.

31. Halliwell B, Gutteridge J. Free Radicals in Biology and Medicine. 4th ed. Oxford and New York: Clarendon Press; 2007:91-94.

32. Ozturk E, Demirbilek S, Begec Z, Surucu M, Fadillioglu E, Kırımlıoglu H, et al. Does leflunomide attenuate the sepsis-induced acute lung injury? Pediatr Surg Int 2008; 24:899-905.

33. Andrades M, Ritter C, de Oliveira MR, Streck EL, Fonseca Moreira JC, Dal-Pizzol F. Antioxidant treatment reverses organ failure in rat model of sepsis: role of antioxidant enzymes imbalance, neutrophil infiltration, and oxidative stress. J Surg Res 2011; 167:e307-e313.

34. Lorente L, Martín MM, Abreu P, Domínguez-Rodriguez A, Labarta L, Díaz C, et al. Sustained high serum malondialdehyde levels are associated with severity and mortality in septic patients. Crit Care 2013; 17:R290.

35. Koksal G, Sayilgan C, Aydin S, Oz H, Uzun H. Correlation of plasma and tissue oxidative stresses in intra-abdominal sepsis. J Surg Res 2004; 122:180-183.

36. Goode HF, Cowley HC, Walker BE, Howdle PD, Webster NR. Decreased antioxidant status and increased lipid peroxidation in patients with septic shock and secondary organ dysfunction. Crit Care Med 1995; 23:646-651.

37. Gadek JE, DeMichele SJ, Karlstad MD, Pacht ER, Donahoe M, Albertson TE, et al. Effect of enteral feeding with eicosapentaenoic acid, gamma-linolenic acid, and antioxidants in patients with acute respiratory distress syndrome. Crit Care Med 1999; 27:1409-1420.

38. Chi C-H, Shiesh S-C, Lin X-Z. Total antioxidant capacity and malondialdehyde in acute abdominal pain. Am J Emerg Med 2002; 20:79-82.

39. Pascual C, Karzai W, Meier-Hellmann A, Oberhoffer M, Horn A, Bredle D, et al. Total plasma antioxidant capacity is not always decreased in sepsis. Crit Care Med 1998; 26:705-709.

40. Tsai K, Hsu T-G, Kong C-W, Lin K-C, Lu F-J. Is the endogenous peroxyl-radical scavenging capacity of plasma protective in systemic inflammatory disorders in humans? Free Radic Biol Med 2000; 28:926-933.