The protective effects of epigallocatechin gallate on lipopolysa ccharide-induced hepatotoxicity: an in vitro study on Hep3B cells

Document Type: Original Article

Authors

1 Department of Medical Microbiology, Medical Faculty, Kafkas University, Kars, Turkey

2 Department of General Surgery, Medical Faculty, Kafkas University, Kars, Turkey

3 Department of Histology and Embryology, Medical Faculty, Kafkas University, Kars, Turkey

4 Department of Pharmacology, Medical Faculty, Ataturk University, Erzurum, Turkey

5 Department of Physiology, Medical Faculty, Kafkas University, Kars, Turkey

6 Department of Biochemistry, Veterinary Faculty, Ataturk University, Erzurum, Turkey

7 Department of Virology, Veterinary Faculty, Ataturk University, Erzurum, Turkey

8 Department of Physiology, Veterinary Faculty, Kafkas University, Kars, Turkey

9 Department of Pharmacology and Toxicology, Veterinary Faculty, Ataturk University, Erzurum, Turkey

Abstract

Objective(s): In the present study, our aim was to investigate the possible protective effects of epigallocatechin gallate (EGCG) on lipopolysaccharide (LPS)-induced hepatotoxicity by using Hep3B human hepatoma cells. Specifically, the study examines the role of some proinflammatory markers and oxidative damage as possible mechanisms of LPS-associated cytotoxicity. Consequently, the hepatocellular carcinoma cell line Hep3B was chosen as a model for investigation of LPS toxicity and the effect of EGCG on LPS-exposed cells.
Materials and Methods:The Hep3B human hepatoma cells were used for this study. The cytotoxic effects of chemicals (EGCG and LPS), AST and ALT levels, SOD and CAT activities, GSH-Px level and TNF-alpha and IL-6 levels were detected by using different biochemical and molecular methods. LPS and EGCG were applied to cells at various times and doses.
Results:The highest treatment dose of EGCG (400 µM) led to a dramatic decrease in SOD level and increase in CAT and GSH levels. Additionally, the highest dose of EGCG also led to a dramatic increase in TNF-alpha and IL-6 levels. On the other hand, effective doses of EGCG (200 and 100 µM) normalized all related parameters levels.
Conclusion:LPS caused hepatotoxicity, but interestingly, a high dose of EGCG was found to be a cytotoxic agent in this study. However, other two doses of EGCG led to a decrease in both inflammatory cytokine levels and antioxidant enzyme levels. Further studies should examine the effect of EGCG on secondary cellular signaling pathways.

Keywords


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