Effect of magnesium sulfate on hyperthermia and pentylen-tetrazol-induced seizure in developing rats

Document Type: Original Article


1 Department of Physiology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Neurophysiology Research Center, Urmia University of Medical Sciences, Urmia, Iran

3 Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran


Objective(s): Febrile seizures (FS) are the most common type of convulsive events among children. Its prevalence has been estimated to be 2-5% in children between 3 months and 5 years old. Also, blood and CSF magnesium levels have been demonstrated to be reduced in children with FS. This study investigates the effect of MgSo4 pretreatment on the behaviors caused by hyperthermia (HT) and effect of these two on pentylen-tetrazol (PTZ)-induced seizure later in life.
Materials and Methods: Thirty two Wistar rats were assigned to 2 groups: saline-hyperthermia-pentylentetrazol (SHP) and magnesium-hyperthermia-pentylentetrazol (MHP). In both groups, HT was induced at the age of 18-19 days old. Before the HT, MHP group received MgSo4 and SHP group received normal saline intraperitoneally (IP). Behaviors of the rats were recorded during the HT. Then, in half of each group (n=8) at the age of 25-26 days old and in other half at the age of 78-79 days, seizure was induced by PTZ.
Results: The HT successfully caused convulsive behaviors in the rats and pretreatment with MgSo4 before HT attenuated HT-induced convulsive behaviors. PTZ-induced seizures a week later was more severe than those of 2 months later.
Conclusion: It can be concluded that pretreatment with MgSO4 inhibits HT-induced seizure and, in a long run, this intervention reduced PTZ-induced seizure later in life.


1. Gholipoor P, Saboory E, Roshan-Milani S, Fereidoni J. Effect of hyperthermia on histamine blood level and convulsive behavior in infant rats. Epilepsy Behav 2013; 29:269-274.

2. Dube CM, Ravizza T, Hamamura M, Zha Q, Keebaugh A, Fok K, et al. Epileptogenesis provoked by prolonged experimental febrile seizures: mechanisms and biomarkers. J Neurosci 2010; 30:7484-7494.

3. Dube CM, Brewster AL, Baram TZ. Febrile seizures: mechanisms and relationship to epilepsy. Brain Dev 2009; 31:366-371.

4. Dube CM, Brewster AL, Richichi C, Zha Q, Baram TZ. Fever, febrile seizures and epilepsy. Trends Neurosci 2007; 30:490-496.

5. Rajab E, Abdeen Z, Hassan Z, Alsaffar Y, Mandeel M, Al Shawaaf F, et al. Cognitive performance and convulsion risk after experimentally-induced febrile-seizures in rat. Int J