Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat

Document Type: Original Article

Authors

1 Postgraduate Student at University of Split School of Medicine, Split, Croatia

2 Department of Medical Chemistry and Biochemistry, University of Split School of Medicine, Split, Croatia

3 Faculty of Kinesiology, University of Split, Split, Croatia

4 Department of Medical Biology, University of Split School of Medicine, Split, Croatia

Abstract

Objective(s): The aim of this study was to estimate effects of hyperbaric (HB) treatment by determination of CD15s and CD11b leukocyte proinflammatory markers expression.  In addition, this study describes changes in CD77 and CD34 expression on rat endothelial cells in renal, pulmonary and cardiac tissue following exposure to hyperbaric pressure.
Materials and Methods:Expression of CD11b and CD15s on leukocytes, as well as CD77 and CD34 expression on endothelial cells in cell suspensions of renal, pulmonary and cardiac tissue in rats after hyperbaric treatment and in control rats were determined by flow cytometry.
Results: Hyperbaric treatment significantly increased percentage of leukocytes expressing CD15s+CD11b- (from 1.71±1.11 to 23.42±2.85, P<0.05). Hyperbaric treatment significantly decreased sum percentage of CD77+CD34- and CD77+CD34+ renal cells (from 16.35±5.5 to 4.48 ±1.28, P<0.05). Hyperbaric treatment significantly increased percentage of CD34+ pulmonary cells (from 3.27±2.01 to 11.92±6.22, P<0.05). Our study is the first reporting the hyperbaric environment influence on CD34+ heart cells in rats.
Conclusion:The current findings of increased percentage of leukocytes expressing endothelial selectin ligand CD15s after hyperbaric treatment, point its role in endothelial damage prevention. We found out a significantly increase in percentage of CD34+ cardiac cells as well as CD34+ pulmonary cells in rats after HB treatment which could be a part of repair mechanism of injured endothelium caused by hyperoxia.

Keywords


1. Madden LA, Chrismas BC, Mellor D, Vince RV, Midgley AW, McNaughton LR, et al. Endothelial function and stress response after simulated dives to 18 msw breathing air or oxygen. Aviat Space Environ Med 2010; 81:41-45.

2. Glavas D, Markotic A, Valic Z, Kovacic N, Palada I, Martinic R, et al. Expression of endothelial selectin ligands on human leukocytes following dive. Exp Biol Med (Maywood) 2008; 233:1181-1188.

3. Maemura K, Fukuda M. Poly-N-acetyllactosaminyl O-glycans attached to leukosialin. The presence of sialyl Le(x) structures in O-glycans. J Biol Chem 1992; 267:24379-2486.

4. Zen K, Cui LB, Zhang CY, Liu Y. Critical role of mac-1 sialyl lewis x moieties in regulating neutrophil degranulation and transmigration. J Mol Biol 2007; 374:54-63.

5. Kalns J, Lane J, Delgado A, Scruggs J, Ayala E, Gutierrez E, et al. Hyperbaric oxygen exposure temporarily reduces Mac-1 mediated functions of human neutrophils. Immunol Lett 2002; 83:125-131.

6. Peschel T, Sixt S, Beitz F, Sonnabend M, Muth G, Thiele H, et al. High, but not moderate frequency and duration of exercise training induces downregulation of the expression of inflammatory and atherogenic adhesion molecules. Eur J Cardiovasc Prev Rehabil  2007; 14:476-482.

7. Chinda D, Nakaji S, Umeda T, Shimoyama T, Kurakake S, Okamura N, et al. A competitive marathon race decreases neutrophil functions in athletes. Luminescence 2003; 18:324-329.

8. Rezic-Muzinic N, Cikes-Culic V, Bozic J, Ticinovic-Kurir T, Salamunic I, Markotic A. Hypercalcemia induces a proinflammatory phenotype in rat leukocytes and endothelial cells. J Physiol Biochem 2013; 69:199-205.

9. Edremitlioglu M, Kilic D, Oter S, Kisa U, Korkmaz A, Coskun O, et al. The effect of hyperbaric oxygen treatment on the renal functions in septic rats: relation to oxidative damage. Surg Today 2005; 35:653-661.

10. Uchida H, Kiyokawa N, Taguchi T, Horie H, Fujimoto J, Takeda T. Shiga toxins induce apoptosis in pulmonary epithelium-derived cells. J Infect Dis 1999; 180:1902-1911..

11. Zoja C, Buelli S, Morigi M. Shiga toxin-associated hemolytic uremic syndrome: pathophysiology of endothelial dysfunction. Pediatr Nephrol 2010; 25:2231-2240.

12. Humphreys BD, Duffield JS, Bonventre JV. Renal stem cells in recovery from acute kidney injury. Minerva Urol Nefrol 2006; 58:329-337.

13. Krause DS, Fackler MJ, Civin CI, May WS. CD34: structure, biology, and clinical utility. Blood 1996; 87:1-13.

14. Nielsen JS, McNagny KM. Novel functions of the CD34 family. J Cell Sci 2008; 121:3683-3692.

15. Laufs U, Werner N, Link A, Endres M, Wassmann S, Jurgens K, et al. Physical training increases endothelial progenitor cells, inhibits neointima formation, and enhances angiogenesis. Circulation 2004; 109:220-226.

16. Heiss C, Keymel S, Niesler U, Ziemann J, Kelm M, Kalka C. Impaired progenitor cell activity in age-related endothelial dysfunction. J Am Coll  Cardiol2005; 45:1441-1448.

17. Imanishi T, Moriwaki C, Hano T, Nishio I. Endothelial progenitor cell senescence is accelerated in both experimental hypertensive rats and patients with essential hypertension. J Hyperten 2005; 23:1831-1837.

18. Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, et al. Dose-dependent contribution of CD34-positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery after myocardial infarction. Circulation 2006; 113:1311-1325.

19. Zhang S, Shpall E, Willerson JT, Yeh ET. Fusion of human hematopoietic progenitor cells and murine cardiomyocytes is mediated by alpha 4 beta 1 integrin/vascular cell adhesion molecule-1 interaction. Circulation Res 2007; 100:693-702.

20. Mackie AR, Losordo DW. CD34-positive stem cells: in the treatment of heart and vascular disease in human beings. Tex Heart Inst J 2011; 38:474-485.

21. Muller AM, Nesslinger M, Skipka G, Muller KM. Expression of CD34 in pulmonary endothelial cells in vivo. Pathobiology 2002; 70:11-17.

22. Culic VC, Kurir TT, Radic S, Zemunik T, Mesaric M, Markotic A. Exposure to hyperbaric pressure alters ganglioside expression in rat liver following partial hepatectomy. Period Biol 2005; 107:267-569.

23. Mogensen CE, Solling. Studies on renal tubular protein reabsorption: partial and near complete inhibition by certain amino acids. Scand J Clin Lab Invest 1977; 37:477-486.

24. Mellembakken JR, Aukrust P, Hestdal K, Ueland T, Abyholm T, Videm V. Chemokines and leukocyte activation in the fetal circulation during preeclampsia. Hypertension 2001; 38:394-398.

25. Park YS, Claybaugh JR, Shiraki K, Mohri M. Renal function in hyperbaric environment. Appl Hum Sci  1998; 17:1-8.

26. Desselle A, Chaumette T, Gaugler MH, Cochonneau D, Fleurence J, Dubois N, et al. Anti-Gb3 monoclonal antibody inhibits angiogenesis and tumor development. PloS One 2012; 7:e45423.

27. Liu L, Mohammadi K, Aynafshar B, Wang H, Li D, Liu J, et al. Role of caveolae in signal-transducing function of cardiac Na+/K+-ATPase. Am J Physiol Cell Physiol 2003; 284:C1550-1560.

28. Markovic-Lipkovski J, Muller CA, Klein G, Flad T, Klatt T, Blaschke S, et al. Neural cell adhesion molecule expression on renal interstitial cells. Nephrol Dial Transplant 2007; 22:1558-1566.

29.Acevedo LM, Londono I, Oubaha M, Ghitescu L, Bendayan M. Glomerular CD34 expression in short- and long-term diabetes. J Histochem Cytochem 2008; 56:605-614.

30. Heyboer M, 3rd, Milovanova TN, Wojcik S, Grant W, Chin M, Hardy KR, et al. CD34+/CD45-dim stem cell mobilization by hyperbaric oxygen - changes with oxygen dosage. Stem Cell Res 2014; 12:638-645.

31. Culic VC, Van Craenenbroeck E, Muzinic NR, Ljubkovic M, Marinovic J, Conraads V, et al. Effects of scuba diving on vascular repair mechanisms. Undersea Hyperb Med 2014; 41:97-104.

32. Dujic Z, Valic Z, Brubakk AO. Beneficial role of exercise on scuba diving. Exerc Sport Sci Rev 2008; 36:38-42.

33. Chamoto K, Gibney BC, Lee GS, Lin M, Collings-Simpson D, Voswinckel R, et al. CD34+ progenitor to endothelial cell transition in post-pneumonectomy angiogenesis. Am J Res Cell Mol Biol 2012; 46:283-289.

34. Blatteis CM. Thermoregulation: Tenth Interna-tional Symposium on the Pharmacology of Thermoregulation. New York: New York Academy of Sciences; 1997.p. 878.

35. Jakob P, Landmesser U. Current status of cell-based therapy for heart failure. Curr Heart Fail Rep 2013; 10:165-176.

36. Massa M, Rosti V, Ferrario M, Campanelli R, Ramajoli I, Rosso R, et al. Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction. Blood 2005; 105:199-206.

37. Takahashi T, Kalka C, Masuda H, Chen D, Silver M, Kearney M, et al. Ischemia- and cytokine-induced mobilization of bone marrow-derived endothelial progenitor cells for neovascularization. Nat Med 1999; 5:434-438.

38. Adams V, Lenk K, Linke A, Lenz D, Erbs S, Sandri M, et al. Increase of circulating endothelial progenitor cells in patients with coronary artery disease after exercise-induced ischemia. Arterioscler Thromb Vasc Biol 2004; 24:684-690.

39. Van Craenenbroeck EM, Vrints CJ, Haine SE, Vermeulen K, Goovaerts I, Van Tendeloo VF, et al. A maximal exercise bout increases the number of circulating CD34+/KDR+ endothelial progenitor cells in healthy subjects. Relation with lipid profile. J Appl Physiol (1985) 2008; 104:1006-1013.

40. Bauwens A, Bielaszewska M, Kemper B, Langehanenberg P, von Bally G, Reichelt R, et al. Differential cytotoxic actions of Shiga toxin 1 and Shiga toxin 2 on microvascular and macrovascular endothelial cells. Thromb Haemost 2011; 105:515-528.

41. Betz J, Bielaszewska M, Thies A, Humpf HU, Dreisewerd K, Karch H, et al. Shiga toxin glycosphingolipid receptors in microvascular and macrovascular endothelial cells: differential association with membrane lipid raft microdomains. J Lipid Res 2011; 52:618-634.

42. Dodelet-Devillers A, Cayrol R, van Horssen J, Haqqani AS, de Vries HE, Engelhardt B, et al. Functions of lipid raft membrane microdomains at the blood-brain barrier. J Mol Med (Berl) 2009; 87:765-774.

43. Park S, Kim JA, Joo KY, Choi S, Choi EN, Shin JA, et al. Globotriaosylceramide leads to K(Ca)3.1 channel dysfunction: a new insight into endothelial dysfunction in Fabry disease. Cardiovasc Res 2011; 89:290-299.

44. Schulz B, Pruessmeyer J, Maretzky T, Ludwig A, Blobel CP, Saftig P, et al. ADAM10 regulates endothelial permeability and T-Cell transmigration by proteolysis of vascular endothelial cadherin. Circ Res 2008; 102:1192-11201.