Effect of chondroitinase ABC on inflammatory and oxidative response following spinal cord injury

Akbari, Mahboobe; Khaksari, Mohammad; Rezaeezadeh-Roukerd, Maryam; Mirzaee, Moghaddameh; Nazari-Robati, Mahdieh

doi:10.22038/ijbms.2017.9016

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Iranian Journal of Basic Medical Sciences

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Effect of chondroitinase ABC on inflammatory and oxidative response following spinal cord injury

Article 11, Volume 20, Issue 7, July 2017, Page 806-812 PDF (992 K)

Document Type: Original Article

DOI: 10.22038/ijbms.2017.9016

Authors

Mahboobe Akbari¹; Mohammad Khaksari²; Maryam Rezaeezadeh-Roukerd³; Moghaddameh Mirzaee⁴; Mahdieh Nazari-Robati

¹^{, 5}

¹Department of Clinical Biochemistry, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

²Endocrinology and Metabolism Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

³Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran

⁴Department of Biostatistics and Epidemiology, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran

⁵Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran

Abstract

Objective(s): Chondroitinase ABC (cABC) treatment improves functional recovery following spinal cord injury (SCI) through degrading inhibitory molecules to axon growth. However, cABC involvement in other pathological processes contributing to SCI remains to be investigated. Here, we studied the effect of cABC I on oxidative stress and inflammation developed in a rat model of SCI.
Materials and Methods: Male rats (220–250 g) were divided into three groups (n=28) including rats that underwent SCI (SCI group), rats subjected to SCI and received an intrathecal injection of phosphate buffer saline (SCI+PBS group), and rats that underwent SCI and received cABC intrathecally (SCI+E group). Then, the level of TNF-α, Il-1β, malondialdehyde, nitric oxide, and myeloperoxidase in injured tissues, as well as hindlimb motor function, were measured at 4 hr, 1, 3 and 7 days post-SCI.
Results: Our data showed that cABC treatment reduced the development of inflammation and oxidative stress associated with SCI at all-time points. In addition, functional recovery was improved in rats that received cABC at 7 days post-SCI.
Conclusion: The present findings indicate that cABC treatment can exert its neuroprotective effect through modulation of post-traumatic inflammatory and oxidative response.

Keywords

Chondroitinase ABC; Functional recovery; Inflammation; Oxidative Stress; Spinal cord injury

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