An Efficient Covalent Coating on Glass Slides for Preparation of Optical Oligonucleotide Microarrays

Document Type: Retraction

Authors

1 Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran 2 Nanobiotechnology center, Department of Chemical Engineering, Jondishapour University of Technology, Dezful, Iran

2 Biomaterials Group (Center of Excellence in Biomaterials), Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran

Abstract

Objective(s): Microarrays are potential analyzing tools for genomics and proteomics researches, which is in needed of suitable substrate for coating and also hybridization of biomolecules.
 
Materials and Methods: In this research, a thin film of oxidized agarose was prepared on the glass slides which previously coated with poly-L-lysine (PLL). Some of the aldehyde groups of the activated agarose linked covalently to PLL amine groups; also bound to the amino groups of biomolecules. These linkages were fixed by UV irradiation. The prepared substrates were compared to only agarose-coated and PLL-coated slides.
 
Results: Results on atomic force microscope (AFM) demonstrated that agarose provided three-dimensional surface which had higher loading and bindig capacity for biomolecules than PLL-coated surface which had two-dimensional surface. In addition, the signal-to-noise ratio in hybridization reactions performed on the agarose-PLL coated substrates increased two fold and four fold compared to agarose and PLL coated substrates, respectively.
 
Conclusion: The agarose-PLL microarrays had the highest signal (2546) and lowest background signal (205) in hybridization, suggesting that the prepared slides are suitable in analyzing wide concentration range of analytes.
 

Keywords


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