Effect of Shark Liver Oil on Peritoneal Murine Macrophages in Responses to Killed-Candida albicans

Document Type: Original Article

Authors

1 Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2 Department of Mycology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Objective(s)
Shark Liver Oil (SLO) is an immunomodulator. Macrophages play a key role in host defense against pathogens like fungi. Candida albicans have mechanisms to escape immune system. We determined the effect of killed-Candida on the in vitro viability of macrophages and the effect of SLO on augmentation of this potency.
Materials and Methods
Peritoneal macrophages were separated and cultured (3*105/well). At first, the effect of killed-Candida (200 cells/well) on macrophage viability was evaluated, using MTT test. Then, MTT was performed on macrophages stimulated with killed-Candida in the presence of SLO.
Results
Killed-Candida suppressed the ability of MTT reduction and hence macrophages viability (P=0.026), but addition of SLO (100 mg/ml) significantly enhanced cell viability (P=0.00). So, SLO could neutralize the inhibitory effect of Candida.
Conclusion
Simultaneous with cytotoxic effect of killed-Candida cells on macrophages viability, SLO augment macrophages viability. So, it can be applied in candidiasis as a complement.

Keywords


1.Marcil A, Harcus D, Thomas DY, Whiteway M. Candida albicans Killing by RAW 264.7 Mouse Macrophage Cells: Effects of Candida Genotype, Infection Ratios, and Gamma Interferon Treatment. Infec Immun 2002; 70:6319-6329.

2.Romani L, Puccetti P, Mencacci A, Cenci E, Spaccapelo R, Tonnetti L, et al. Neutralization of IL-10 up- regulates nitric oxide production and protects susceptible mice from challenge with C.Albicans. J Immunol 1994; 152:3514-21.

3.Cenci E, Romani L, Mencacci A, Spaccapelo R, Tonnetti L, Grohmann U, et al. Interleukin-4 and interleukin- 10 inhibit nitric oxidedependent macrophage killing of Candida albicans. Eur JImmunol 1993; 23:1034-8.

4.Schroppel K, Kryk M, Herrman M, Leberer E, Rollinghoff M, Bogdan Ch. Suppression of type 2 NO-synthase activity in macrophages by Candida albicans. Int J Med Microbiol 2001; 290, 659-668.

5.Lorsbach RB, Russell SW. A specific sequence of stimulation is required to induce synthesis of the antimicrobial molecule nitric oxide by mouse macrophages. Infect Immun 1992; 60: 2133- 2135.

6.Chinen T, Qureshi MH, Koguchi Y, Kawakami K. Candida albicans suppresses nitric oxide (NO) production by interferon-gamma (IFN-g) and lipopoly saccharide (LPS)-stimulated murine peritoneal macrophages. Clin Exp Immunol 1999; 115:491-497. 

7.Danley DL, Higler AE, Winkel CA. Generation of hydrogen peroxide by Candida albicans and influence on murine polymorphonuclear leukocyte activity. Infect Immun. 1983; 40:97-102.

8.Lewkowicz N, Lewkowicz P, Kurnatowska A, Tchorzewski H. Biological action and clinical application of shark liver oil. Pol Merkur Lekarski 2006; 20:598-601.

9.Pugliese PT, Jordan K, Cederberg H, Brohult J. Some biological actions of alkylglycerols from shark liver oil. J Altern Complement Med 1998; 4:87-99.

10.Szostak WB, Szostak-Wegierek D. Health properties of shark oil. Przegl Lek 2006; 63:223-6.

11.Kinchington D, Kngro H, Jeffires DJ. Design and testing of antiviral compounds. In:Dsselberger U. (Ed), Medical virology: a practical approach, IRL Press; New York; 1995; p.147-72.

12.Kinchington D, Kngro H, Jeffires DJ. Design and testing of antiviral compounds. In: Dsselberger U. (Ed), Medical virology: a practical approach, IRL Press; New York; 1995; p.147-72.

13.Ribeiro-Dias F, Russo M, Nascimento FRF, Barbuto JAM, Timenetsky J, Jancar S. Thioglycollate-elicited murinemacrophages are cytotoxic to Mycoplasma arginini-infected YAC-1 tumor cells. Braz J Med Biol Res. 1998; 31: 1425-1428.

14.Murciano C, Villamon E, Connor JE O, Gozalbo D, Luisa MG. Killed Candida albicans Yeasts and Hyphae Inhibit Gamma Interferon Release by Murine Natural Killer Cells.Infect Immun 2006; 74: 1403-1406.

15.Marodi L, Scheiber S, Anderson DC, MacDermott RP, Kochak HM, Johnston RB. Enhancement of macrophage candidacidal activity by interferon-gamma. Increased phagocytosis, killing, and calcium signal mediated by a decreased number of mannose receptors. J Clin Investig 1993; 91:2596-2601.