Ultrasound medicine in the era of precision theranostics: Mechanisms, molecular strategies, and clinical translation

1. Bader KB, Padilla F, Haworth KJ, Ellens N, Dalecki D, Miller DL, et al. Overview of therapeutic ultrasound applications and safety considerations: 2024 update. J Ultrasound Med 2025; 44: 381-433. 
2. Ren J, Li J, Chen S, Liu Y, Ta D. Unveiling the potential of ultrasound in brain imaging: Innovations, challenges, and prospects. Ultrasonics 2025; 145: 107465. 
3. Uddin SMZ, Komatsu DE, Motyka T, Petterson S. Low-intensity continuous ultrasound therapies—a systematic review of current  state-of-the-art and future perspectives. J Clin Med 2021; 10: 2698. 
4. Zhang C, Zhang Q, Xu Q, Jiang X, Ma Y, Liu C, et al. Ultrasound targeted microbubbles for theranostic applications in liver diseases:  From molecular imaging to targeted therapy. Drug Deliv 2025; 32: 2541656. 
5. Jackson SS, Le HM, Kerkhof DL, Corrado GD. Point-of-care ultrasound, the new musculoskeletal physical examination. Curr Sports Med Rep 2021; 20: 109-112. 
6. Glass C, Sarwal A, Zavitz J, Nitsche J, Joyner J, Johnson LL, et al. Scoping review of implementing a longitudinal curriculum in undergraduate medical education: The wake forest experience. Ultrasound J 2021; 13: 23. 
7. Haskins SC, Bronshteyn Y, Perlas A, El-Boghdadly K, Zimmerman J, Silva M, et al. American society of regional anesthesia and pain medicine expert panel  recommendations on point-of-care ultrasound education and training for regional anesthesiologists and pain physicians-part I: Clinical indications. Reg Anesth Pain Med 2021; 46: 1031-1047. 
8. Cid-Serra X, Hoang W, El-Ansary D, Canty D, Royse A, Royse C. Clinical impact of point-of-care ultrasound in internal medicine inpatients: A  systematic review. Ultrasound Med Biol 2022; 48: 170-179. 
9. Dudek M, Szarpak L, Peacock FW, Gasecka A, Michalski T, Wroblewski P, et al. Diagnostic performance of point-of-use ultrasound of resuscitation outcomes: A  systematic review and meta-analysis of 3265 patients. Cardiol J 2023; 30: 237-246. 
10. Demir ZEF, Sheybani ND. Therapeutic ultrasound for multimodal cancer treatment: A spotlight on breast  cancer. Annu Rev Biomed Eng 2025; 27: 371-402. 
11. Fang K, Lei J, Zhao Y, Feng Y, Huang H, Hou C. Progress and application of multifunctional ultrasound theranostic agents. Curr Pharm Biotechnol 2025.
12. Zhu K, Wang J, Wang Z, Chen Q, Song J, Chen X. Ultrasound-activated theranostic materials and their bioapplications. Angew Chemie Int Ed 2025; 64: e202422278. 
13. Du J, Liao M, Zhang D, Li X. Advanced strategies for ultrasound control and applications in sonogenetics and  gas vesicle-based technologies: A review. Int J Nanomedicine 2025; 20: 6533-6549. 
14. Xu X, Cao J, Mu Y, Zhang H, Wang YL, Chen M, et al. Ultrasound-induced nitric oxide-propelled nanomotor for multimodal theranostics  of cancer with deep penetration and extended lifetime. Adv Sci 2025; 12: e16709. 
15. Zheng Q, Xia B, Huang X, Luo J, Zhong S, Li X. Nanomedicines for high‑intensity focused ultrasound cancer treatment and theranostics (Review). Exp Ther Med 2023; 25: 170. 
16. Zhang B, Mo L, Huang R, Chen G, Yang S, Zhang X, et al. Ultrasound-enabled sonogenetics: Pioneering advances in cancer theranostics. Adv Funct Mater 2025; 35: 2418748. 
17. Martínez-Fernández R, Paschen S, del Álamo M, Rodríguez-Rojas R, Pineda-Pardo JA, Blesa J, et al. Focused ultrasound therapy for movement disorders. Lancet Neurol 2025; 24: 698-712. 
18. Zhu Z, Zhang Z, Qi G, Li Y, Li Y, Mu L. A dual-branch network for ultrasound image segmentation. Biomed Signal Process Control 2025; 103: 107368. 
19. Luijten B, Chennakeshava N, Eldar YC, Mischi M, van Sloun RJG. Ultrasound signal processing: from models to deep learning. Ultrasound Med Biol 2023; 49: 677-698. 
20. Yang T, Karakus O, Anantrasirichai N, Achim A. Current advances in computational lung ultrasound imaging: A review. IEEE Trans Ultrason Ferroelectr Freq Control 2023; 70: 2-15. 
21. Izadifar Z, Babyn P, Chapman D. Mechanical and biological effects of ultrasound: A review of present knowledge. Ultrasound Med Biol 2017; 43: 1085-1104. 
22. Dalecki D. Mechanical bioeffects of ultrasound. Annu Rev Biomed Eng 2004; 6: 229-248. 
23. Nightingale K. Acoustic radiation force impulse (ARFI) imaging: A review. Curr Med Imaging Rev 2011; 7: 328-339. 
24. Doherty JR, Trahey GE, Nightingale KR, Palmeri ML. Acoustic radiation force elasticity imaging in diagnostic ultrasound. IEEE Trans Ultrason Ferroelectr Freq Control 2013; 60: 685-701. 
25. Mohammadjavadi M, Ash RT, Glover GH, Pauly KB. Optimization of MR acoustic radiation force imaging (MR-ARFI) for human transcranial focused ultrasound. Magn Reson Med 2025; 94: 1060-1071. 
26. Odéen H, Payne AH, Parker DL. Magnetic resonance acoustic radiation force imaging (MR-ARFI). J Magn Reson Imaging 2025; 62: 20-39. 
27. Church CC, Labuda C, Nightingale K. A theoretical study of inertial cavitation from acoustic radiation force impulse  imaging and implications for the mechanical index. Ultrasound Med Biol 2015; 41: 472-485. 
28. Fowlkes JB, Holland CK. Mechanical bioeffects from diagnostic ultrasound: AIUM consensus statements.  American institute of ultrasound in medicine. J ultrasound Med  Off J Am Inst  Ultrasound Med 2000; 19: 69-72. 
29. Quarato CMI, Lacedonia D, Salvemini M, Tuccari G, Mastrodonato G, Villani R, et al. A review on biological effects of ultrasounds: Key messages for clinicians. Diagnostics (Basel, Switzerland) 2023; 13: 855. 
30. Delalande A, Kotopoulis S, Postema M, Midoux P, Pichon C. Sonoporation: Mechanistic insights and ongoing challenges for gene transfer. Gene 2013; 525: 191-199. 
31. Helfield BL, Chen X, Qin B, Watkins SC, Villanueva FS. Mechanistic insight into sonoporation with ultrasound-stimulated polymer  microbubbles. Ultrasound Med Biol 2017; 43: 2678-2689. 
32. Jiang A, Wang Z, Song D, Zhang X, Guan M, Li X, et al. The application of ultrasound-induced blood–brain barrier opening in neurology and immunology. Small 2025; 21: 2502699. 
33. Gionso M, Herlin E, Uva L, Guidi F, Tortoli P, Durando G, et al. Ultrasound guided blood brain barrier opening using a diagnostic probe in a whole brain model. Sci Rep 2025; 15: 10674. 
34. Sun T, Samiotaki G, Wang S, Acosta C, Chen CC, Konofagou EE. Acoustic cavitation-based monitoring of the reversibility and permeability of  ultrasound-induced blood-brain barrier opening. Phys Med Biol 2015; 60: 9079-9094. 
35. Şen T, Tüfekçioğlu O, Koza Y. Mechanical index. Anatol J Cardiol 2015; 15: 334-336. 
36. Bachu VS, Kedda J, Suk I, Green JJ, Tyler B. High-intensity focused ultrasound: A review of mechanisms and clinical applications. Ann Biomed Eng 2021; 49: 1975-1991. 
37. Shrivastava D, Vaughan JT. A generic bioheat transfer thermal model for a perfused tissue. J Biomech Eng 2009; 131: 74506. 
38. van Rhoon GC, Samaras T, Yarmolenko PS, Dewhirst MW, Neufeld E, Kuster N. CEM43°C thermal dose thresholds: A potential guide for magnetic resonance  radiofrequency exposure levels? Eur Radiol 2013; 23: 2215-2227. 
39. Santos MA, Goertz DE, Hynynen K. Focused ultrasound hyperthermia mediated drug delivery using thermosensitive  liposomes and visualized with in vivo two-photon microscopy. Theranostics 2017; 7: 2718-2731. 
40. Hijnen N, Kneepkens E, de Smet M, Langereis S, Heijman E, Grüll H. Thermal combination therapies for local drug delivery by magnetic resonance-guided high-intensity focused ultrasound. Proc Natl Acad Sci 2017; 114: E4802-4811.
41. Zhong C, Bai J, Yang X, Ji Y, Huang J, Tan X, et al. A study of saponin-encapsulated ultrasound microbubbles Rb 3 NPs@MBs for atherosclerosis targeted treatment. Biomater Sci 2025; 13: 4984-5000.
42. Tian S, Jiang T, Su M, Su P, Jia Z, Ke L, et al. Novel supramolecular self-assembled nanomedicines for ultrasound-triggered tumor programmed therapy. Chem Eng J 2025; 520: 166106. 
43. Cai L, Du J, Han F, Shi T, Zhang H, Lu Y, et al. Piezoelectric metal-organic frameworks based sonosensitizer for enhanced nanozyme  catalytic and sonodynamic therapies. ACS Nano 2023; 17: 7901-7910. 
44. Karthika V, Badrinathan Sridharan, Nam JW, Kim D, Gyun Lim H. Neuromodulation by nanozymes and ultrasound during Alzheimer’s disease management. J Nanobiotechnology 2024; 22: 139. 
45. Cağlı M, Duyur Çakıt B, Pervane S. Efficacy of therapeutic ultrasound added to complex decongestive therapy in breast cancer-related lymphedema. Lymphat Res Biol 2025; 23: 272-280.
46. Yang Y, Sun Y, Mao W wei, Zhang H, Ni B, Jiang L. Oxidative stress induces downregulation of TP53INP2 and suppresses osteogenic differentiation of BMSCs during osteoporosis through the autophagy degradation pathway. Free Radic Biol Med 2021; 166: 226-237. 
47. Hu Y, Zhao G, Qin L, Yu Z, Zhang M, Ma X, et al. Trans, trans-2, 4-Decadienal induces endothelial cell injury by impairing mitochondrial function and autophagic flux. Food Funct 2021; 12: 5488-5500. 
48. Al Refaai KA, AlSawaftah NA, Abuwatfa W, Husseini GA. Drug release via ultrasound-activated nanocarriers for cancer treatment: A review. Pharmaceutics 2024; 16: 1383. 
49. Fokong S, Theek B, Wu Z, Koczera P, Appold L, Jorge S, et al. Image-guided, targeted and triggered drug delivery to tumors using polymer-based  microbubbles. J Control release Off J Control Release  Soc 2012; 163: 75-81. 
50. Mørch Ý, Hansen R, Berg S, Åslund AKO, Glomm WR, Eggen S, et al. Nanoparticle-stabilized microbubbles for multimodal imaging and drug delivery. Contrast Media Mol Imaging 2015; 10: 356-366. 
51. Lv Y, Hao L, Hu W, Ran Y, Bai Y, Zhang L. Novel multifunctional pH-sensitive nanoparticles loaded into microbubbles as drug delivery vehicles for enhanced tumor targeting. Sci Rep 2016; 6: 29321. 
52. Chuang CF, Lin CW, Yeh CK. Ultrasound-triggered drug release and cytotoxicity of microbubbles with diverse drug attributes. Ultrason Sonochem 2025; 112: 107182. 
53. Han X, Wang F, Shen J, Chen S, Xiao P, Zhu Y, et al. Ultrasound nanobubble coupling agent for effective noninvasive deep‐layer drug delivery. Adv Mater 2024; 36: 2306993. 
54. Cui R, Zhou J, Yang W, Chen Y, Chen L, Tan L, et al. Ultrasound-triggered nanogel boosts chemotherapy and immunomodulation in  colorectal cancer. ACS Appl Mater Interfaces 2025; 17: 211-221. 
55. Nittayacharn P, Abenojar E, Cooley MB, Berg FM, Counil C, Sojahrood AJ, et al. Efficient ultrasound-mediated drug delivery to orthotopic liver tumors–Direct comparison of doxorubicin-loaded nanobubbles and microbubbles. J Control Release 2024; 367: 135-147. 
56. Furusawa Y, Hassan MA, Zhao QL, Ogawa R, Tabuchi Y, Kondo T. Effects of therapeutic ultrasound on the nucleus and genomic DNA. Ultrason Sonochem 2014; 21: 2061-2068. 
57. Przystupski D, Ussowicz M. Landscape of cellular bioeffects triggered by ultrasound-induced sonoporation. Int J Mol Sci 2022; 23: 11222. 
58. Elsner HI, Lindblad EB. Ultrasonic degradation of DNA. DNA 1989; 8: 697-701. 
59. Costello M, Pugh TJ, Fennell TJ, Stewart C, Lichtenstein L, Meldrim JC, et al. Discovery and characterization of artifactual mutations in deep coverage targeted  capture sequencing data due to oxidative DNA damage during sample preparation. Nucleic Acids Res 2013; 41: e67. 
60. Harle J, Mayia F, Olsen I, Salih V. Effects of ultrasound on transforming growth factor-beta genes in bone cells. Eur Cell Mater 2005; 10: 70-76. 
61. Hasanova GI, Noriega SE, Mamedov TG, Thakurta SG, Turner JA, Subramanian A. The effect of ultrasound stimulation on the gene and protein expression of chondrocytes seeded in chitosan scaffolds. J Tissue Eng Regen Med 2011; 5: 815-822. 
62. Filieri S, Miciaccia M, Armenise D, Baldelli OM, Liturri A, Ferorelli S, et al. Can focused ultrasound overcome the failure of chemotherapy in treating pediatric  diffuse intrinsic pontine glioma due to a blood-brain barrier obstacle? Pharmaceuticals (Basel) 2025; 18: 525. 
63. McKee JR, Christman CL, O’Brien Jr WD, Wang SY. Effects of ultrasound on nucleic acid bases. Biochemistry 1977; 16: 4651-4654. 
64. Pinamonti S, Caruso A, Mazzeo V, Zebini E, Rossi A. DNA damage from pulsed sonication of human leukocytes in vitro. IEEE Trans Ultrason Ferroelectr Freq Control 1986; 33 :179-185. 
65. Miller DL, Reese JA, Frazier ME. Single strand DNA breaks in human leukocytes induced by ultrasound in vitro. Ultrasound Med Biol 1989; 15: 765-771. 
66. Miller DL, Thomas RM, Frazier ME. Ultrasonic cavitation indirectly induces single strand breaks in DNA of viable cells in vitro by the action of residual hydrogen peroxide. Ultrasound Med Biol 1991; 17: 729-735. 
67. Miller DL, Thomas RM, Frazier ME. Single strand breaks in CHO cell DNA induced by ultrasonic cavitation in vitro. Ultrasound Med Biol 1991;17: 401–406. 
68. Kondo T, Arai S, Kuwabara M, Yoshii G, Kano E. Damage in DNA irradiated with 1.2 MHz ultrasound and its effect on template  activity of DNA for RNA synthesis. Radiat Res 1985; 104: 284-292. 
69. Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S, et al. GammaH2AX and cancer. Nat Rev Cancer 2008; 8: 957-967. 
70. Ward IM, Chen J. Histone H2AX is phosphorylated in an ATR-dependent manner in response to  replicational stress. J Biol Chem 2001; 276: 47759-47762. 
71. Stiff T, O’Driscoll M, Rief N, Iwabuchi K, Löbrich M, Jeggo PA. ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to  ionizing radiation. Cancer Res 2004; 64: 2390–2396. 
72. Furusawa Y, Fujiwara Y, Campbell P, Zhao QL, Ogawa R, Hassan MA, et al. DNA double-strand breaks induced by cavitational mechanical effects of ultrasound  in cancer cell lines. PLoS One 2012; 7: e29012. 
73. Liu S, Li M, Guo Z, Chen Z. Exploring the molecular mechanism of cancer radiosensitization: The impact of physical stimulation therapy. Strahlentherapie und Onkol 2025; 201: 1058-1070.
74. Abdollahi A, Domhan S, Jenne JW, Hallaj M, Dell’Aqua G, Mueckenthaler M, et al. Apoptosis signals in lymphoblasts induced by focused ultrasound. FASEB J Off Publ Fed Am Soc Exp Biol 2004; 18: 1413-1414. 
75. Furusawa Y, Kondo T. DNA damage induced by ultrasound and cellular responses. Mol Biol 2017; 6: 2. 
76. Chen X, Wan JMF, Yu ACH. Sonoporation as a cellular stress: Induction of morphological repression and  developmental delays. Ultrasound Med Biol 2013; 39: 1075-1086. 
77. Hassan MA, Furusawa Y, Minemura M, Rapoport N, Sugiyama T, Kondo T. Ultrasound-induced new cellular mechanism involved in drug resistance. PLoS One 2012; 7: e48291. 
78. Leone P, Malerba E, Susca N, Favoino E, Perosa F, Brunori G, et al. Endothelial cells in tumor microenvironment: Insights and perspectives. Front Immunol 2024; 15: 1367875. 
79. Li Z, Zhang B, Duan S, Liu R, Wang Y, Wang Y, et al. Ultrasound-activated nanovesicles for adenosine exhaustion and immune checkpoint blockade in cancer immunotherapy. J Control Release 2025; 385: 113988. 
80. Anderson NM, Simon MC. The tumor microenvironment. Curr Biol 2020; 30: R921-925. 
81. Wang MJ, Graf-Alexiou ML, Nguyen DKCT, Hoang MTH, Major PP, Le PLH. A study of accuracy and reliability of intraoral ultrasound using ex vivo and in vivo data. Oral Surg Oral Med Oral Pathol Oral Radiol. 2025; 139: e96. 
82. Park K, Veena MS, Shin DS. Key players of the immunosuppressive tumor microenvironment and emerging  therapeutic strategies. Front cell Dev Biol 2022; 10: 830208. 
83. Zhang J, Luan X, Lv Z, Wang Y, Yu Q, Zhong H, et al. Electron transfer driven piezocatalytic degradation of emerging contaminants: Recent advances, modification strategies, and prospects. J Water Process Eng 2025; 75: 108012. 
84. Musiu C, Lupo F, Agostini A, Lionetto G, Bevere M, Paiella S, et al. Cellular collusion: Cracking the code of immunosuppression and chemo resistance  in PDAC. Front Immunol 2024; 15: 1341079. 
85. Singh A, Tijore A, Margadant F, Simpson C, Chitkara D, Low BC, et al. Enhanced tumor cell killing by ultrasound after microtubule depolymerization. Bioeng Transl Med 2021; 6: e10233. 
86. Tijore A, Margadant F, Dwivedi N, Morgan L, Yao M, Hariharan A, et al. Ultrasound-mediated mechanical forces activate selective tumor cell apoptosis. Bioeng Transl Med 2025; 10: e10737. 
87. Lejbkowicz F, Zwiran M, Salzberg S. The response of normal and malignant cells to ultrasound in vitro. Ultrasound Med Biol 1993; 19: 75–82. 
88. Huang S, Cheng C, Wang Z, Li R, Li W, Yu K, et al. Low-intensity-pulsed-ultrasound-treated menstrual-blood-derived mesenchymal stem cells repair endometrial injury by PI3K/AKT pathway inhibition. Reprod Biomed Online 2025; 50: 104486. 
89. Xie X, Zhang J, Wang Y, Shi W, Tang R, Tang Q, et al. Nanomaterials augmented bioeffects of ultrasound in cancer immunotherapy. Mater Today Bio 2024; 24: 100926. 
90. Zhang J, Sun L, Jiang L, Xie X, Wang Y, Wu R, et al. Regulation of CTLs/Tregs via highly stable and ultrasound-responsive cerasomal nano-modulators for enhanced colorectal cancer immunotherapy. Adv Sci 2024; 11: 2400485. 
91. Baez A, Singh D, He S, Hajiaghayi M, Gholizadeh F, Darlington PJ, et al. Immunomodulation of human T cells by microbubble-mediated focused ultrasound. Front Immunol 2024; 15: 1486744. 
92. Campanelli R, Carolei A, Catarsi P, Abbà C, Boveri E, Paulli M, et al. Circulating polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) have a biological role in patients with primary myelofibrosis. Cancers 2024; 16: 2556. 
93. Zhou LX, Jiang YZ, Li XQ, Zhang JM, Li SP, Wei L, et al. Myeloid-derived suppressor cells-induced exhaustion of CD8 + T-cell participates in rejection after liver transplantation. Cell Death Dis 2024; 15: 507. 
94. Pellegatta S, Corradino N, Zingarelli M, Porto E, Gionso M, Berlendis A, et al. The immunomodulatory effects of fluorescein-mediated sonodynamic treatment lead to systemic and intratumoral depletion of myeloid-derived suppressor cells in a preclinical malignant glioma model. Cancers 2024; 16: 792. 
95. Huang D, Xu M, Wang H, Zhao Y, Zhang Z, Yu M, et al. SIRPα blockade therapy potentiates immunotherapy by inhibiting PD-L1+ myeloid cells in hepatocellular carcinoma. Cell Death Dis 2025; 16: 451. 
96. Huang Z, Yue JQ, Zhu RH, Xin JY, Luo HC, Li KY. Perfluorobutane-enhanced US Targeting M2 Tumor-associated Macrophages for Predicting Programmed Cell Death-1 Response in Hepatocellular Carcinoma. Radiol Imaging Cancer 2025; 7: e240472. 
97. Alva A, Kim C, Premdas P, Ferry Y, Lee H, Lal N, et al. Imaging of macrophage accumulation in solid tumors with ultrasound. Nat Commun 2025; 16: 6322. 
98. Fu Z, Zhang L, Chen R, Zhan J, Zhong J, Zheng W, et al. Biphasic co-detection of melanoma aneuploid tumor cells and tumor endothelial cells in guidance of specifying the field cancerized surgical excision margin and administering immunotherapy. Cancer Lett 2024; 598: 217099. 
99. Chinigò G, Scarpellino G, Petrillo S, Genova T, Ruffinatti FA, Munaron L. Modulation of purinergic signaling in endothelial cells by tumor microenvironment. Vascul Pharmacol 2024; 155: 107311. 
100. Sadeghipour N, Tabesh F, Natarajan A, Lutz A, Paulmurugan R, Kaffas A El. Molecular ultrasound imaging of PD-L1 expression on cancer endothelial cells. Ultrasound Med Biol 2025; 51: 1675-1681.
101. Changizi S, Marquette IG, VanSant J, Alghazwat O, Elgattar A, Liao Y, et al. Carbon monoxide release from ultrasound-sensitive microbubbles improves endothelial cell growth. J Biomed Mater Res Part A 2024; 112: 600–612. 
102. Mai Z, Lin Y, Lin P, Zhao X, Cui L. Modulating extracellular matrix stiffness: A strategic approach to boost cancer immunotherapy. Cell Death Dis 2024; 15: 307. 
103. Lin N, Yang Z, Yi W, Chen Y, Lai F. Delving into immune modulation: Thymectomy in myasthenia gravis. J Cardiothorac Surg 2025; 20: 277. 
104. Guipaud O, Lago C, Portier L, Paget V, François A, Supiot S, et al. Exploiting the endothelial-immune axis to improve radiotherapy efficacy. Br J Radiol 2025; 98: 1176-1187. 
105. He M, Zhu H, Dong J, Lin W, Li B, Li Y, et al. Low-intensity pulsed ultrasound improves metabolic dysregulation in obese mice by suppressing inflammation and extracellular matrix remodeling. Ultrasonics 2025; 145: 107488. 
106. Huang R, Zhang B, Chen G, Zhao Y, Wang R, Zhu H, et al. Ultrasound-mediated FAK-targeted nano-sapper for tumor extracellular matrix remodeling to potentiate cancer immunotherapy. Chem Eng J 2025; 515: 163837. 
107. Yilmaz AÇ, Toktas H, Celik S, Sen S. Therapeutic ultrasound modulates cell proliferation and proinflammatory cytokine levels in osteoarthritic chondrocytes. J Cell Mol Med 2025; 29: e70257. 
108. de Araújo Alves CC, de Melo PF, Vieira L, Mathur S, Burtin C, Maldaner VZ, et al. Early detection of muscle wasting assessed by ultrasound and analysis of growth factor and systemic inflammation mediators in critically ill trauma patients: An observational study. Eur J Trauma Emerg Surg 2025; 51: 93. 
109. Zhang C, Chen Y, Han J, Liu R, Liu C, Zhao Y, et al. Ultrasound nanobubble-based combinational strategies of loaded miR-107-3p and CD133 Ab for anti-PD-L1 and anti-hepatocellular cancer stem cells. Int J Pharm 2025; 670: 125140. 
110. Yamaguchi T, Endo-Takahashi Y, Awaji K, Numazawa S, Onishi Y, Tada R, et al. Microfluidic nanobubbles produced using a micromixer for ultrasound imaging and gene delivery. Sci Rep 2025; 15: 14871. 
111. Patil C, Priyanka R, Harshitha BM, Oshik S, Yashwanth S, Darshan BR, et al. Advanced nanotheranostic approaches for targeted glioblastoma treatment: A synergistic fusion of CRISPR-Cas gene editing, AI-driven tumor profiling, and BBB-modulation. Med Oncol 2025; 42: 413. 
112. Hazel K, Singh D, He S, Guertin Z, Husser MC, Helfield B. Focused ultrasound and microbubble-mediated delivery of CRISPR-Cas9 ribonucleoprotein to human induced pluripotent stem cells. Mol Ther 2025; 33: 986-996. 
113. Song J, Parakhonskiy B V, Skirtach AG. Energy transfer influence on superfast calcium carbonate synthesis: Using microwave heating, ultrasound cavitation and mechanical stirring. J Mater Res Technol 2025; 35: 5600–5613. 
114. Careaga J, Nikolić V, Said-Houari B. Westervelt-based modeling of ultrasound-enhanced drug delivery. J Nonlinear Sci 2025; 35: 61. 
115. Sharma D, Czarnota GJ. Using ultrasound and microbubble to enhance the effects of conventional cancer therapies in clinical settings. Cancer Metastasis Rev 2025; 44: 39. 
116. Han L, Dai Q, He C, Xu J, Cui L, Xie X, et al. A tetrahedral DNA nanoplatform with ultrasound-triggered biomimetic nanocarriers for targeted siMCM2 delivery and reversal of imatinib resistance in gastrointestinal stromal tumors. Chem Eng J 2025; 504: 158843. 
117. Kaushik A, Fabiilli ML, Myers DD, Fowlkes JB, Aliabouzar M. Advancing acoustic droplet vaporization for tissue characterization using quantitative ultrasound and transfer learning. IEEE Trans Biomed Eng 2025; 72: 1897-1908. 
118. Song Y, Wang Y, Wang W, Xie Y, Zhang J, Liu J, et al. Advancements in noninvasive techniques for transplant rejection: From biomarker detection to molecular imaging. J Transl Med 2025; 23: 147. 
119. Morawski AM, Lanza GA, Wickline SA. Targeted contrast agents for magnetic resonance imaging and ultrasound. Curr Opin Biotechnol 2005; 16: 89–92. 
120. Weller GER, Lu E, Csikari MM, Klibanov AL, Fischer D, Wagner WR, et al. Ultrasound imaging of acute cardiac transplant rejection with microbubbles  targeted to intercellular adhesion molecule-1. Circulation 2003; 108:2 18–224. 
121. Jin Y, Gao P, Liang L, Wang Y, Li J, Wang J, et al. Noninvasive quantification of granzyme B in cardiac allograft rejection using  targeted ultrasound imaging. Front Immunol 2023; 14: 1164183. 
122. Hou L, Guo Z, Liu S, Liang X, Du M, Chen Z. Ultrasound molecular imaging with VEGFR2 Targeted microbubbles to evaluate intrauterine adhesion after endometrial injury and monitor the therapeutic effects. J Ultrasound Med 2025; 44: 1555–1568. 
123. Liu J, Chen Y, Wang G, Lv Q, Yang Y, Wang J, et al. Ultrasound molecular imaging of acute cardiac transplantation rejection using  nanobubbles targeted to T lymphocytes. Biomaterials 2018; 162: 200–207. 
124. Liang M, Kang X, Liu H, Zhang L, Wang T, Ye M, et al. Ultrasound-energized OX40L-expressing biohybrid for multidimensional mobilization of sustained T cell-mediated antitumor immunity and potent sono-immunotherapy. J Am Chem Soc 2025; 147: 13833–13850. 
125. Li J, Wen L, Guo Y, Yao D, Sun B, Mou H, et al. Combination of low-intensity pulsed ultrasound irradiating immune organs with immune checkpoint blockade augments systemic anti-tumor immunity on low tumor burden 4T-1 breast cancer. Cancer Immunol Immunother 2025; 74: 281. 
126. Liao T, Liu X, Ren J, Zhang H, Zheng H, Li X, et al. Noninvasive and quantitative measurement of C4d deposition for the diagnosis of  antibody-mediated cardiac allograft rejection. EBioMedicine 2018; 37: 236–245. 
127. Kardani H, Vaghani H, Patel P, Kumbhani J. Green synthesis of hydrazone-linked 1,2,3-triazole-coumarin hybrids via ultrasound: In vitro and in silico DNA gyrase inhibition studies. ChemistrySelect 2025; 10: e02939. 
128. Mahmood U, Josephson L. Molecular MR imaging probes. Proc IEEE 2005; 93: 800–808. 
129. Aherne T, Tscholakoff D, Finkbeiner W, Sechtem U, Derugin N, Yee E, et al. Magnetic resonance imaging of cardiac transplants: The evaluation of rejection of  cardiac allografts with and without immunosuppression. Circulation 1986; 74: 145-156. 
130. Maheshwari S, Singh A, Verma A, Shariq M, Akhtar J, Alsaidan OA, et al. Superparamagnetic iron oxide nanoparticles (SPIONs) in targeting brain tumors: Advances and challenges. Med Oncol 2025 ; 42: 338. 
131. Wu YL, Ye Q, Foley LM, Hitchens TK, Sato K, Williams JB, et al. In situ labeling of immune cells with iron oxide particles: An approach to detect  organ rejection by cellular MRI. Proc Natl Acad Sci U S A 2006; 103: 1852–1857. 
132. Toki D, Zhang W, Hor KLM, Liuwantara D, Alexander SI, Yi Z, et al. The role of macrophages in the development of human renal allograft fibrosis in  the first year after transplantation. Am J Transplant 2014; 14: 2126-2136. 
133. Salehi S, Reed EF. The divergent roles of macrophages in solid organ transplantation. Curr Opin Organ Transplant 2015; 20: 446-453. 
134. Ye Q, Wu YL, Foley LM, Hitchens TK, Eytan DF, Shirwan H, et al. Longitudinal tracking of recipient macrophages in a rat chronic cardiac allograft rejection model with noninvasive magnetic resonance imaging using micrometer-sized paramagnetic iron oxide particles. Circulation 2008; 118: 149-156. 
135. Dolan RS, Rahsepar AA, Blaisdell J, Suwa K, Ghafourian K, Wilcox JE, et al. Multiparametric cardiac magnetic resonance imaging can detect acute cardiac allograft rejection after heart transplantation. JACC Cardiovasc Imaging 2019; 12: 1632-1641. 
136. Liu L, Ye Q, Wu Y, Hsieh WY, Chen CL, Shen HH, et al. Tracking T-cells in vivo with a new nano-sized MRI contrast agent. Nanomedicine 2012; 8: 1345–1354. 
137. Giannakodimos I, Kaltsas A, Moulavasilis N, Kratiras Z, Mitropoulos D, Chrisofos M, et al. Fusion MRI/ultrasound-guided transperineal biopsy: A game changer in prostate  cancer diagnosis. J Clin Med 2025; 14: 453. 
138. Ge J, Zhang Q, Zeng J, Gu Z, Gao M. Radiolabeling nanomaterials for multimodality imaging: New insights into nuclear  medicine and cancer diagnosis. Biomaterials 2020; 228: 119553. 
139. Eichendorff S, Svendsen P, Bender D, Keiding S, Christensen EI, Deleuran B, et al. Biodistribution and PET imaging of a novel [68Ga]-anti-CD163-antibody conjugate  in rats with collagen-induced arthritis and in controls. Mol imaging Biol 2015; 17: 87-93. 
140. Fiordelisi MF, Auletta L, Meomartino L, Basso L, Fatone G, Salvatore M, et al. Preclinical molecular imaging for precision medicine in breast cancer mouse models. Contrast Media Mol Imaging 2019; 2019: 8946729. 
141. O’Neill ASG, Terry SYA, Brown K, Meader L, Wong AMS, Cooper JD, et al. Non-invasive molecular imaging of inflammatory macrophages in allograft  rejection. EJNMMI Res 2015; 5: 69. 
142. Li H, Chen Y, Jin Q, Wu Y, Deng C, Gai Y, et al. Noninvasive radionuclide molecular imaging of the CD4-positive T lymphocytes in acute cardiac rejection. Mol Pharm 2021; 18: 1317–1326. 
143. Sharif-Paghaleh E, Yap ML, Meader LL, Chuamsaamarkkee K, Kampmeier F, Badar A, et al. Noninvasive imaging of activated complement in ischemia-reperfusion injury  post-cardiac transplant. Am J Transplant 2015; 15: 2483–2490. 
144. Bhatnagar A, Narula J. Radionuclide imaging of cardiac pathology: A mechanistic perspective. Adv Drug Deliv Rev 1999; 37: 213-223. 
145. Grabner A, Kentrup D, Edemir B, Sirin Y, Pavenstädt H, Schlatter E, et al. PET with 18F-FDG-labeled T lymphocytes for diagnosis of acute rat renal allograft  rejection. J Nucl Med 2013; 54: 1147–1153. 
146. Konishi M, Erdem SS, Weissleder R, Lichtman AH, McCarthy JR, Libby P. Imaging granzyme B activity assesses immune-mediated myocarditis. Circ Res 2015; 117: 502–512. 
147. Larimer BM, Wehrenberg-Klee E, Dubois F, Mehta A, Kalomeris T, Flaherty K, et al. Granzyme B PET imaging as a predictive biomarker of immunotherapy response. Cancer Res 2017; 77: 2318–2327. 
148. Schwenck J, Sonanini D, Cotton JM, Rammensee HG, la Fougère C, Zender L, et al. Advances in PET imaging of cancer. Nat Rev Cancer 2023; 23: 474–490. 
149. Daly KP, Dearling JLJ, Seto T, Dunning P, Fahey F, Packard AB, et al. Use of [18F]FDG positron emission tomography to monitor the development of  cardiac allograft rejection. Transplantation 2015; 99: e132-139. 
150. Ueno T, Dutta P, Keliher E, Leuschner F, Majmudar M, Marinelli B, et al. Nanoparticle PET-CT detects rejection and immunomodulation in cardiac allografts. Circ Cardiovasc Imaging 2013; 6: 568–573. 
151. Lohmann P, Schäfer L, Krause S, Altunay B, Willuweit A, Werner JM, et al. Advancements in non-invasive visualization of the immune environment in glioblastoma: A systematic review. Neuro-Oncology Adv 2025; 7: vdaf176. 
152. Taşdelen E, Kutlay NY, Kaplan İ, Altıner Ş, Alay MT. Association between OX40L rs1234314 and rs844648 polymorphisms and unexplained recurrent pregnancy loss. Mol Biol Rep 2025; 52: 548. 
153. Hirai T, Mayer AT, Nobashi TW, Lin PY, Xiao Z, Udagawa T, et al. Imaging alloreactive T cells provides early warning of organ transplant  rejection. JCI insight 2021; 6: e145360. 
154. Dar O, Dulay MS, Riesgo-Gil F, Morley-Smith A, Brookes P, Lyster H, et al. Cardiac transplant rejection assessment with 18F-FDG PET-CT: Initial  single-centre experience for diagnosis and management. EJNMMI Rep 2024; 8: 9. 
155. Li B, Zhao X, Luo S, Zhong Q, Zhao H, Du C, et al. Preoperative localization of parathyroid glands in secondary hyperparathyroidism: Correlations between 99mTc-MIBI-SPECT/CT, ultrasound, and pathological characteristics. Clin Kidney J 2025; 18: sfaf040. 
156. Liu T, Wang J, Cui T, Yang L, Li Z, Yan F. Targeted biosynthetic nanobubbles for ultrasound molecular imaging of prostate cancer. Ultrasound Med Biol 2025; 51: 1447–1458. 
157. Sheth M, Knight C, Wu Q, Vasilyeva A, Upadhyay A, Bau L, et al. Size matters: Micro- versus nanobubbles in ultrasound imaging and therapy. Sci Adv 2025; 11: eads2177. 
158. Wegierak D, Nittayacharn P, Cooley MB, Berg FM, Kosmides T, Durig D, et al. Nanobubble contrast enhanced ultrasound imaging: A review. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2024; 16: e2007. 
159. Kulkarni AD, Mukarrama T, Barlow BR, Kim J. Recent advances in non-invasive in vivo tracking of cell-based cancer immunotherapies. Biomater Sci 2025; 13: 1939–1959. 
160. Cen P, Luo X, Wang J, Chen H, Tian M, Zhang H. Molecular imaging of stem cell therapies in ischemic stroke. Eur J Nucl Med Mol Imaging 2025; 53: 71-92.
161. Leder T, Seifert P, Gühne F, Freesmeyer M. Simultaneous identification of Tc-99m-sestamibi-positive autonomous thyroid adenoma and adjacent F-18-ethylcholine-positive parathyroid adenoma in patient with graves’ disease using real-time ultrasound fusion imaging. Diagnostics 2025; 15: 1262. 
162. Sriwastwa A, Trout AT, Mahoney BW, Wang LL, Scheler JL. Nuclear medicine imaging in epilepsy. RadioGraphics 2025; 45: e240062. 
163. Zamani-Siahkali N, Mirshahvalad SA, Farbod A, Divband G, Pirich C, Veit-Haibach P, et al. SPECT/CT, PET/CT, and PET/MRI for response assessment of bone metastases. Semin Nucl Med 2024; 54: 356–3570. 
164. Yang L, Gao T, Huang Y, Wang P he, Han X hao, Wu J, et al. Ultrasound-targeted β-catenin gene therapy improves the cardiac function in mice after myocardial infarction. Cardiovasc Toxicol 2025; 25: 74–84. 
165. Tang S, McGinnis R, Cao Z, Baker JR, Xu Z, Wang S. Ultrasound-guided histotripsy triggers the release of tumor-associated antigens from breast cancers. Cancers 2025; 17: 183. 
166. Wang L, Cao L, Shao K, Su J, Li G, Wang C, et al. Phytochlorin-based sonosensitizers combined with free-field ultrasound for immune-sonodynamic cancer therapy. Adv Mater 2025; 37: 2410559. 
167. Casenghi M, Poletti E, Popolo Rubbio A, Brambilla N, Testa L, Bedogni F, et al. [Transcatheter aortic valve implantation for pure aortic regurgitation]. G Ital Cardiol (Rome) 2021; 22: 21S-28S. 
168. Wu Q, Xia Y, Xiong X, Duan X, Pang X, Zhang F, et al. Focused ultrasound-mediated small-molecule delivery to potentiate immune checkpoint blockade in solid tumors. Front Pharmacol 2023; 14: 1169608. 
169. Liu S, Zhang Y, Liu Y, Wang W, Gao S, Yuan W, et al. Ultrasound-targeted microbubble destruction remodels tumour microenvironment to improve immunotherapeutic effect. Br J Cancer 2023; 128: 715-725. 
170. Liu D, Ling Y, Dong L, Zhang J, Li X, Chen X, et al. Ultrasound-triggered drug-loaded nanobubbles for enhanced T cell recruitment in cancer chemoimmunotherapy. Biomaterials 2025; 317: 123086. 
171. Deng J, Zhang Y, Feng J, Wu F. Dendritic cells loaded with ultrasound-ablated tumour induce in vivo specific antitumour immune responses. Ultrasound Med Biol 2010; 36: 441–448. 
172. Liao Y, Wang D, Yang X, Ni L, Lin B, Zhang Y, et al. High‑intensity focused ultrasound thermal ablation boosts the efficacy of immune  checkpoint inhibitors in advanced cancers with liver metastases: A single‑center retrospective cohort study. Oncol Lett 2025; 29: 124. 
173. Hawley JJ, Allen SL, Thompson DM, Schwarz AJ, Tranquart FJM. Commercially available ultrasound contrast agents: Factors contributing to favorable outcomes with ultrasound-mediated drug delivery and ultrasound localization microscopy imaging. Invest Radiol 2025; 60: 813-822.
174. Exner AA, Escoffre JM. Editorial: Biomedical advances in ultrasound-mediated drug/molecule delivery. Front Pharmacol 2022; 13: 974921. 
175. Cai X, Liu Y, Luo G, Yu Z, Jiang C, Xu C. Ultrasound-assisted immunotherapy for malignant tumour. Front Immunol 2025; 16: 1547594. 
176. Unga J, Hashida M. Ultrasound induced cancer immunotherapy. Adv Drug Deliv Rev 2014; 72: 144–153. 
177. Yan  Ruiqian, Li  Haixia, Gao  Junxi. Relationship between the ultrasound features of different molecular subtypes of breast cancer and positive PD-1/PD-L1 expression. J Int Med Res 2025; 53: 03000605251314812. 
178. Dahan M, Cortet M, Lafon C, Padilla F. Combination of focused ultrasound, immunotherapy, and chemotherapy. J Ultrasound Med 2022; 42: 559-573. 
179. Hu C, Li H, Deng T, Liu Z, Yang L, Peng L, et al. Abscopal effect of focused ultrasound combined immunotherapy in animal solid  tumor model: A systematic reviews and meta-analysis. Front Immunol 2024; 15: 1474343. 
180. Joiner JB, Pylayeva-Gupta Y, Dayton PA. Focused ultrasound for immunomodulation of the tumor microenvironment. J Immunol 2020; 205: 2327-2341. 
181. Zhou Y, Pang L, Ding T, Chen K, Liu J, Wu M, et al. Precise in situ delivery of a photo-enhanceable inflammasome-activating nanovaccine activates anticancer immunity. Cancer Res 2024; 84: 3834–3847. 
182. Zhang Y, Deng J, Feng J, Wu F. Enhancement of antitumor vaccine in ablated hepatocellular carcinoma by high-intensity focused ultrasound. World J Gastroenterol 2010; 16: 3584–3591. 
183. Ma J, Yuan H, Zhang J, Sun X, Yi L, Li W, et al. An ultrasound-activated nanoplatform remodels tumor microenvironment through diverse cell death induction for improved immunotherapy. J Control Release 2024; 370: 501-515. 
184. Sethuraman SN, Singh MP, Patil G, Li S, Fiering S, Hoopes PJ, et al. Novel calreticulin-nanoparticle in combination with focused ultrasound induces immunogenic cell death in melanoma to enhance antitumor immunity. Theranostics 2020; 10: 3397-3412. 
185. von Eckstaedt H V, Weng K, Sacksen I, Stovall R, Grivas P, Bhatia S, et al. Sonographic signatures of immune checkpoint inhibitor-associated musculoskeletal adverse events. Cancers 2025; 17: 2344. 
186. Abe S, Nagata H, Crosby EJ, Inoue Y, Kaneko K, Liu CX, et al. Combination of ultrasound-based mechanical disruption of tumor with immune checkpoint blockade modifies tumor microenvironment and augments systemic antitumor immunity. J Immunother Cancer 2022; 10: e003717. 
187. Yilmaz M, Karaaslan M, Şirin ME, Aybal HÇ, Polat ME, Soyturk S, et al. Efficacy of low-intensity pulsed ultrasound (LIPUS) in the treatment of erectile dysfunction: A systematic review. Int Urol Nephrol 2025; 58: 77-89.
188. Bai Y, Li X, Wu K, Heng BC, Zhang X, Deng X. Biophysical stimuli for promoting bone repair and regeneration. 2025; 5: 1-22. 
189. Kearney CJ, Hsu HP, Spector M. The use of extracorporeal shock wave-stimulated periosteal cells for orthotopic bone generation. Tissue Eng Part A 2012; 18: 1500–1508. 
190. Wu S, Xu X, Sun J, Zhang Y, Shi J, Xu T. Low-intensity pulsed ultrasound accelerates traumatic vertebral fracture healing  by coupling proliferation of type H microvessels. J Ultrasound Med  2018; 37: 1733–1742. 
191. Wang X, Lin Q, Zhang T, Wang X, Cheng K, Gao M, et al. Low-intensity pulsed ultrasound promotes chondrogenesis of mesenchymal stem cells via regulation of autophagy. Stem Cell Res Ther 2019; 10: 41. 
192. Liao Q, Li BJ, Li Y, Xiao Y, Zeng H, Liu JM, et al. Low-intensity pulsed ultrasound promotes osteoarthritic cartilage regeneration by BMSC-derived exosomes via modulating the NF-κB signaling pathway. Int Immunopharmacol 2021; 97: 107824. 
193. Zhou J, Zhu Y, Ai D, Zhou M, Li H, Fu Y, et al. Low-intensity pulsed ultrasound regulates osteoblast-osteoclast crosstalk via EphrinB2/EphB4 signaling for orthodontic alveolar bone remodeling. Front Bioeng Biotechnol 2023; 11: 1192720. 
194. Ying S, Tan M, Feng G, Kuang Y, Chen D, Li J, et al. Low-intensity pulsed ultrasound regulates alveolar bone homeostasis in experimental periodontitis by diminishing oxidative stress. Theranostics 2020; 10: 9789–9807. 
195. Teoh KH, Whitham R, Wong JF, Hariharan K. The use of low-intensity pulsed ultrasound in treating delayed union of fifth  metatarsal fractures. Foot (Edinb) 2018; 35: 52–55. 
196. Schofer MD, Block JE, Aigner J, Schmelz A. Improved healing response in delayed unions of the tibia with low-intensity  pulsed ultrasound: Results of a randomized sham-controlled trial. BMC Musculoskelet Disord 2010; 11: 229. 
197. Farkash U, Bain O, Gam A, Nyska M, Sagiv P. Low-intensity pulsed ultrasound for treating delayed union scaphoid fractures:  Case series. J Orthop Surg Res 2015; 10: 72. 
198. Simpson A, Keenan G, Nayagam S, Atkins RM, Marsh D, Clement ND. Low-intensity pulsed ultrasound does not influence bone healing by distraction osteogenesis: a multicentre double-blind randomised control trial. Bone Joint J 2017; 99: 494–502. 
199. Salem KH, Schmelz A. Low-intensity pulsed ultrasound shortens the treatment time in tibial distraction  osteogenesis. Int Orthop 2014; 38: 1477–1482. 
200. Sun S, Tang L, Zhao T, Kang Y, Sun L, Liu C, et al. Longitudinal effects of low-intensity pulsed ultrasound on osteoporosis and  osteoporotic bone defect in ovariectomized rats. Ultrasonics 2021; 113: 106360. 
201. Wada K, Kawano M, Hemmi Y, Suzuki R, Kunoki K, Sakagami H, et al. Effect of low-intensity pulsed ultrasound on healing of bone defects in rat tibia  as measured by reconstructed three-dimensional analysis of micro CT images. In Vivo 2022; 36: 643–648. 
202. Wang Y, Qiu Y, Li J, Zhao C, Song J. Low-intensity pulsed ultrasound promotes alveolar bone regeneration in a periodontal injury model. Ultrasonics 2018; 90: 166–172. 
203. Zeng Q, Qi X, Shi G, Zhang M, Haick H. Wound dressing: From nanomaterials to diagnostic dressings and healing  evaluations. ACS Nano 2022; 16: 1708–1733. 
204. Zhao Y, Wang S, Ding Y, Zhang Z, Huang T, Zhang Y, et al. Piezotronic effect-augmented Cu(2-x)O-BaTiO(3) sonosensitizers for  multifunctional cancer dynamic therapy. ACS Nano 2022; 16: 9304–9316. 
205. Pang X, He X, Qiu Z, Zhang H, Xie R, Liu Z, et al. Targeting integrin pathways: Mechanisms and advances in therapy. Signal Transduct Target Ther 2023; 8: 1. 
206. Chaudhuri O, Cooper-White J, Janmey PA, Mooney DJ, Shenoy VB. Effects of extracellular matrix viscoelasticity on cellular behaviour. Nature 2020; 584: 535–546. 
207. Xia P, Shen S, Lin Q, Cheng K, Ren S, Gao M, et al. Low-intensity pulsed ultrasound treatment at an early osteoarthritis stage  protects rabbit cartilage from damage via the integrin/focal adhesion kinase/mitogen-activated protein kinase signaling pathway. J Ultrasound Med 2015; 34: 1991–1999. 
208. Pascoal S, Monteiro F, Oliveira S, Simoni A, Carvalho Ó, Pinho T. Biomodulation effects induced by ultrasound stimulation in periodontal cells implicated in orthodontic tooth movement: A systematic review. Orthod Craniofac Res 2025; 28: 54–66. 
209. Jing L, Fan S, Yao X, Zhang Y. Effects of compound stimulation of fluid shear stress plus ultrasound on stem  cell proliferation and osteogenesis. Regen Biomater 2021; 8: rbab066. 
210. Zhang G, Li X, Wu L, Qin YX. Piezo1 channel activation in response to mechanobiological acoustic radiation  force in osteoblastic cells. Bone Res 2021; 9: 16. 
211. Zhou H, Dong Y, Wu Z, Peng X, Yan M, Chen S, et al. Ultrasound-assisted enzyme extraction of dendrobium officinale polysaccharides: Extraction process, characterization, immunomodulatory effects. Ultrason Sonochem 2025; 114: 107248. 
212. Svenskaya YI, Genina EA, Parakhonskiy B V, Lengert E V, Talnikova EE, Terentyuk GS, et al. A simple non-invasive approach toward efficient transdermal drug delivery based  on biodegradable particulate system. ACS Appl Mater Interfaces 2019; 11: 17270-17282. 
213. Su M, Li C, Deng S, Xu L, Shan Z, Xing Y, et al. Balance between the CMC/ACP nanocomplex and blood assimilation orchestrates  immunomodulation of the biomineralized collagen matrix. ACS Appl Mater Interfaces 2023; 15: 58166–58180. 
214. Newton JM, Hanoteau A, Liu HC, Gaspero A, Parikh F, Gartrell-Corrado RD, et al. Immune microenvironment modulation unmasks therapeutic benefit of radiotherapy  and checkpoint inhibition. J Immunother Cancer 2019; 7: 216. 
215. Hoefsmit EP, Rozeman EA, Van TM, Dimitriadis P, Krijgsman O, Conway JW, et al. Comprehensive analysis of cutaneous and uveal melanoma liver metastases. J Immunother Cancer 2020; 8: e001501. 
216. Reilley MJ, Morrow B, Ager CR, Liu A, Hong DS, Curran MA. TLR9 activation cooperates with T cell checkpoint blockade to regress poorly  immunogenic melanoma. J Immunother Cancer 2019; 7: 323. 
217. Tang S, Tang R, Chen G, Zhang D, Lin K, Yang H, et al. Personalized neoantigen hydrogel vaccine combined with PD-1 and CTLA-4 double  blockade elicits antitumor response in liver metastases by activating intratumoral CD8+CD69+ T cells. J Immunother Cancer 2024; 12: e009543. 
218. Xu J, Wang H, Xu L, Chao Y, Wang C, Han X, et al. Nanovaccine based on a protein-delivering dendrimer for effective antigen  cross-presentation and cancer immunotherapy. Biomaterials 2019; 207: 1-9. 
219. Gou S, Wang S, Liu W, Chen G, Zhang D, Du J, et al. Adjuvant-free peptide vaccine targeting Clec9a on dendritic cells can induce  robust antitumor immune response through Syk/IL-21 axis. Theranostics 2021; 11: 7308-7321. 
220. Yan S, Wang D, Zhang L, Gan T, Yao H, Zhu H, et al. LIPUS-S/B@NPs regulates the release of SDF-1 and BMP-2 to promote stem cell  recruitment-osteogenesis for periodontal bone regeneration. Front Bioeng Biotechnol 2023; 11: 1226426. 
221. Zhang N, Chow SKH, Leung KS, Cheung WH. Ultrasound as a stimulus for musculoskeletal disorders. J Orthop Transl 2017; 9: 52–59. 
222. Butler S, Ashcroft K, Arrowsmith S, Griffiths R, Studd A. Assessment of thermal index compliance in clinical ultrasound examinations. Ultrasound 2024; 32: 151–156. 
223. Wu J, Xie F, Lof J, Sayyed S, Porter TR. Utilization of modified diagnostic ultrasound and microbubbles to reduce myocardial infarct size. Heart 2015; 101: 1468–1474. 
224. Drukker L, Droste R, Chatelain P, Noble JA, Papageorghiou AT. Safety indices of ultrasound: Adherence to recommendations and awareness during  routine obstetric ultrasound scanning. Ultraschall Med 2020; 41: 138–145. 
225. Abbott JG. Rationale and derivation of MI and TI—a review. Ultrasound Med Biol 1999; 25: 431–441. 
226. Zhu Y, Zhang Q, Wang Y, Liu W, Zeng S, Yuan Q, et al. Identification of necroptosis and immune infiltration in heart failure through bioinformatics analysis. J Inflamm Res 2025; 18: 2465–2481. 
227. Lentacker I, De Cock I, Deckers R, De Smedt SC, Moonen CTW. Understanding ultrasound induced sonoporation: Definitions and underlying mechanisms. Adv Drug Deliv Rev 2014; 72: 49–64. 
228. Tomizawa M, Shinozaki F, Motoyoshi Y, Sugiyama T, Yamamoto S, Sueishi M. Sonoporation: Gene transfer using ultrasound. World J Methodol 2013; 3: 39–44. 
229. Liang J, Chen L, Li Y, Chen Y, Yuan L, Qiu Y, et al. Unraveling the prefrontal cortex-basolateral amygdala pathway’s role on  schizophrenia’s cognitive impairments: A multimodal study in patients and mouse models. Schizophr Bull 2024; 50: 913–923. 
230. Birdi J, Heymans S V., Collado-Lara G, Van Den Abeele K, D’hooge J, Bertrand A. Single-shot attenuation coefficient estimation for ultrasound contrast agents. Front Phys 2022; 10: 1-21. 
231. Bao MH, Lv QL, Li HG, Zhang YW, Xu BF, He BS. A novel putative role of TNK1 in atherosclerotic inflammation implicating the  Tyk2/STAT1 pathway. Mediators Inflamm 2020; 2020: 6268514. 
232. Elmekki H, Islam S, Alagha A, Sami H, Spilkin A, Zakeri E, et al. Comprehensive review of reinforcement learning for medical ultrasound imaging. Artif Intell Rev 2025; 58: 284. 
233. Ting SG, Lea-Banks H, Hynynen K. Physical characterization to improve scalability and potential of anesthetic-loaded nanodroplets. Pharmaceutics 2023; 15: 2077. 
234. Martin KH, Dayton PA. Current status and prospects for microbubbles in ultrasound theranostics. Wiley Interdiscip Rev Nanomed Nanobiotechnol 2013; 5: 329–345. 
235. Gawne PJ, Ferreira M, Papaluca M, Grimm J, Decuzzi P. New opportunities and old challenges in the clinical translation of  nanotheranostics. Nat Rev Mater 2023; 8: 783–798. 
236. Kiessling F, Fokong S, Koczera P, Lederle W, Lammers T. Ultrasound microbubbles for molecular diagnosis, therapy, and theranostics. J Nucl Med 2012; 53: 345–348. 
237. Stern AD. Innovation under regulatory uncertainty: Evidence from medical technology. J Public Econ. 2017; 145: 181–200. 
238. Kiessling F, Fokong S, Bzyl J, Lederle W, Palmowski M, Lammers T. Recent advances in molecular, multimodal and theranostic ultrasound imaging. Adv Drug Deliv Rev 2014; 72: 15-27. 
239. Oddo L, Cerroni B, Domenici F, Bedini A, Bordi F, Chiessi E, et al. Next generation ultrasound platforms for theranostics. J Colloid Interface Sci 2017; 491: 151–160. 
240. Yan L, Li Q, Fu K, Zhou X, Zhang K. Progress in the application of artificial intelligence in ultrasound-assisted  medical diagnosis. Bioeng (Basel, Switzerland) 2025; 12: 288. 
241. Chen Y, Yang H, Pan H, Siddiqui F, Verdone A, Zhang Q, et al. BURExtract-Llama: An LLM for Clinical Concept Extraction in Breast Ultrasound Reports. In: Proceedings of the 1st International Workshop on Multimedia Computing for Health and Medicine. New York, NY, USA: Association for Computing Machinery; 2024. p. 53-58. 
242. Xu X, Sankar R. Large language model agents for biomedicine: a comprehensive review of methods, evaluations, challenges, and future directions. Information 2025; 16: 894. 
243. Wang C, He T, Zhou H, Zhang Z, Lee C. Artificial intelligence enhanced sensors - enabling technologies to  next-generation healthcare and biomedical platform. Bioelectron Med 2023; 9: 17. 
244. Puccetti M, Pariano M, Schoubben A, Giovagnoli S, Ricci M. Biologics, theranostics, and personalized medicine in drug delivery systems. Pharmacol Res 2024; 201: 107086. 
245. Zou F, Liu Y, Luo Y, Xu T. A wearable spatiotemporal controllable ultrasonic device with amyloid-β disaggregation for continuous Alzheimer’s disease therapy. Sci Adv 2025; 11: eadw1732. 
246. Sergeeva O V., Luo L, Guiseppi-Elie A. Cancer theragnostics: Closing the loop for advanced personalized cancer treatment through the platform integration of therapeutics and diagnostics. Front Bioeng Biotechnol 2025; 12: 1499474. 
247. Xie R, Wang Y, Gong S. External stimuli-responsive nanoparticles for spatially and temporally controlled  delivery of CRISPR-Cas genome editors. Biomater Sci 2021; 9: 6012–6022. 
248. Liu Z, Si L, Shi S, Li J, Zhu J, Lee WH, et al. Classification of three anesthesia stages based on near-infrared spectroscopy  signals. IEEE J Biomed Heal informatics 2024; 28: 5270–5279. 
249. Hu F, Yang H, Qiu L, Wang X, Ren Z, Wei S, et al. Innovation networks in the advanced medical equipment industry: Supporting  regional digital health systems from a local-national perspective. Front public Heal 2025; 13: 1635475. 
250. Johanssen VA, Ruan JL, Vince O, Thomas A, Peeters S, Soto MS, et al. Targeted opening of the blood-brain barrier using VCAM-1 functionalised  microbubbles and “whole brain” ultrasound. Theranostics 2024; 14: 4076–4089. 
251. Kaufmann BA, Sanders JM, Davis C, Xie A, Aldred P, Sarembock IJ, et al. Molecular imaging of inflammation in atherosclerosis with targeted ultrasound detection of vascular cell adhesion molecule-1. Circulation 2007; 116: 276–284. 
252. Langbein T, Weber WA, Eiber M. Future of theranostics: An outlook on precision oncology in nuclear medicine. J Nucl Med 2019; 60: 13S-19S. 
253. Pandit-Taskar N, Modak S. Norepinephrine transporter as a target for imaging and therapy. J Nucl Med 2017; 58: 39S-53S. 
254. Gawne PJ, Bryant HE, DuBois SG, George SL, Gray J, Knox L, et al. Theranostics for neuroblastoma: Making molecular radiotherapy work better. J Nucl Med 2025; 66: 490–496. 
255. Eberlein U, Cremonesi M, Lassmann M. Individualized dosimetry for theranostics: necessary, nice to have, or  counterproductive? J Nucl Med 2017; 58: 97S-103S. 
256. Nagarajah J, Janssen M, Hetkamp P, Jentzen W. Iodine symporter targeting with (124)I/(131)I theranostics. J Nucl Med 2017; 58: 34S-38S. 
257. Lin G, Mu Q, Revia R, Stephen Z, Jeon M, Zhang M. A highly selective iron oxide-based imaging nanoparticle for long-term monitoring  of drug-induced tumor cell apoptosis. Biomater Sci 2021; 9: 471–481. 
258. Jiang F, Liu S, Wang L, Chen H, Huang Y, Cao Y, et al. ROS-responsive nanoprobes for bimodal imaging-guided cancer targeted  combinatorial therapy. Int J Nanomedicine 2024; 19: 8071–8090. 
259. Jolesz FA. MRI-guided focused ultrasound surgery. Annu Rev Med 2009; 60: 417–430. 
260. Shen YT, Chen L, Yue WW, Xu HX. Artificial intelligence in ultrasound. Eur J Radiol 2021; 139: 109717. 
261. Du Y, Chen L, Yan MC, Wang YL, Zhong XL, Xv CX, et al. Neurometabolite levels in the brains of patients with autism spectrum disorders: A meta-analysis of proton magnetic resonance spectroscopy studies (N = 1501). Mol Psychiatry 2023; 28: 3092-3103. 
262. Kayarian F, Patel D, O’Brien JR, Schraft EK, Gottlieb M. Artificial intelligence and point-of-care ultrasound: Benefits, limitations, and  implications for the future. Am J Emerg Med 2024; 80: 119–122. 
263. Jiang Y, Zhang L, Liu Z, Wang L. The value of handheld ultrasound in point-of-care or at home EF prediction. Acta Cardiol 2025; 80: 979-985. 
264. Chen L, Jiang Z, Yang L, Fang Y, Lu S, Akakuru OU, et al. HPDA/Zn as a CREB inhibitor for ultrasound imaging and stabilization of atherosclerosis plaque. Chinese J Chem 2023; 41: 199–206.
265. Kim S, Fischetti C, Guy M, Hsu E, Fox J, Young SD. Artificial intelligence (AI) applications for point of care ultrasound (POCUS) in  low-resource settings: A scoping review. Diagnostics (Basel, Switzerland) 2024; 14: 1669. 
266. Akkus Z, Cai J, Boonrod A, Zeinoddini A, Weston AD, Philbrick KA, et al. A survey of deep-learning applications in ultrasound: Artificial intelligence–powered ultrasound for improving clinical workflow. J Am Coll Radiol 2019; 16: 1318-1328. 
267. Luan S, Yu X, Lei S, Ma C, Wang X, Xue X, et al. Deep learning for fast super-resolution ultrasound microvessel imaging. Phys Med Biol 2023; 68: 245023. 
268. Yu X, Luan S, Lei S, Huang J, Liu Z, Xue X, et al. Deep learning for fast denoising filtering in ultrasound localization microscopy. Phys Med Biol 2023; 68: 205002. 
269. Li B, Enichen EJ, Heydari K, Kvedar JC. Artificial intelligence guided imaging as a tool to fill gaps in health care  delivery. NPJ Digit Med 2025; 8: 248. 
270. Liu Z, Li M, Xie Q, Liu Y, Huang J, Zeng Q, et al. Eradicating fungal biofilm-based infections by ultrasound-assisted semiconductor  sensitized upconversion photodynamic therapy. Nat Commun 2025; 16: 6499. 
271. Baloescu C, Bailitz J, Cheema B, Agarwala R, Jankowski M, Eke O, et al. Artificial intelligence-guided lung ultrasound by nonexperts. JAMA Cardiol 2025; 10: 245-253. 
272. Komatsu M, Sakai A, Dozen A, Shozu K, Yasutomi S, Machino H, et al. Towards clinical application of artificial intelligence in ultrasound imaging. Biomedicines 2021; 9: 720. 
273. Wu GG, Zhou LQ, Xu JW, Wang JY, Wei Q, Deng YB, et al. Artificial intelligence in breast ultrasound. World J Radiol 2019; 11: 19-26. 
274. Jiang Z, Chen Z, Xu Y, Li H, Li Y, Peng L, et al. Low-frequency ultrasound sensitive piezo1 channels regulate keloid-related  characteristics of fibroblasts. Adv Sci 2024; 11: e2305489. 
275. García-Herreros S, López Gómez JJ, Cebria A, Izaola O, Salvador Coloma P, Nozal S, et al. Validation of an artificial intelligence-based ultrasound imaging system for  quantifying muscle architecture parameters of the rectus femoris in disease-related malnutrition (DRM). Nutrients 2024; 16: 1806. 
276. Firuzpour F, Saleki K, Aram C, Rezaei N. Nanocarriers in glioblastoma treatment: A neuroimmunological perspective. Rev Neurosci 2024; 36: 431-453.
277. Rahimi M, Fattahi A. Acidity enhancement of α-carbon of beta diketones via hydroxyl substituents: A density functional theory study. J Phys Org Chem 2021; 34: e4157.
278. Omata D, Unga J, Suzuki R, Maruyama K. Lipid-based microbubbles and ultrasound for therapeutic application. Adv Drug Deliv Rev 2020; 154–155: 236–44. 
279. Aram C, Firuzpour F, Barancheshmeh M, Kamali MJ. Unveiling the translational and therapeutic potential of small interfering RNA molecules in combating SARS-CoV-2: A review. Int J Biol Macromol 2025; 318: 145203. 
280. Cai L, Pfob A. Artificial intelligence in abdominal and pelvic ultrasound imaging: Current applications. Abdom Radiol 2025; 50: 1775–1789. 
281. Voelker R. Cardiac ultrasound uses artificial intelligence to produce images. JAMA 2020; 323: 1034. 
282. Fraga S, Brandão A, Soares ME, Morais T, Duarte JA, Pereira L, et al. Short- and long-term distribution and toxicity of gold nanoparticles in the rat  after a single-dose intravenous administration. Nanomedicine 2014; 10: 1757–1766. 
283. Zhang XD, Luo Z, Chen J, Song S, Yuan X, Shen X, et al. Ultrasmall glutathione-protected gold nanoclusters as next generation  radiotherapy sensitizers with high tumor uptake and high renal clearance. Sci Rep 2015; 5: 8669. 
284. Ali MRK, Rahman MA, Wu Y, Han T, Peng X, Mackey MA, et al. Efficacy, long-term toxicity, and mechanistic studies of gold nanorods photothermal therapy of cancer in xenograft mice. Proc Natl Acad Sci 2017; 114: E3110–3118.
285. Wang X, Xie N, Zhang H, Zhou W, Lei J. Isoorientin ameliorates macrophage pyroptosis and atherogenesis by reducing KDM4A  levels and promoting SKP1-Cullin1-F-box E3 ligase-mediated NLRP3 ubiquitination. Inflammation 2025; 48: 3629-3648.
286. Chen L, Cruz E, Oikari LE, Padmanabhan P, Song J, Götz J. Opportunities and challenges in delivering biologics for Alzheimer’s disease by low-intensity ultrasound. Adv Drug Deliv Rev 2022; 189: 114517. 
287. Wan Y, Shen Y, Wang J, Zhang T, Fu X. Knowledge mapping of ultrasound technology and triple-negative breast cancer: A  visual and bibliometric analysis. Discov Oncol 2025; 16: 1248.