Sulfur dioxide reduces hippocampal cells death and improves learning and memory deficits in rat model of transient global ischemia/reperfusion

Document Type : Original Article


1 Neurobiomedical Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

2 Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

3 School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

4 Laboratory of Learning and Memory, Physiology Research Center and Physiology Department, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran

5 Addiction Research Center, Shahroud University of Medical Sciences, Shahroud, Iran


Objective(s): According to recent the findings, sulfur dioxide (SO2) is produced by the cardiovascular system, influencing some major biological processes. Based on previous research, SO2 exhibits antioxidant effects and inhibits apoptosis following cardiac ischemia/reperfusion. Therefore, the objective of the current study was to examine the neuroprotective impact of SO2 following global cerebral ischemia/reperfusion (I/R).
Materials and Methods: Forty-eight male Wistar rats that weighed 260–300 g, were randomly allocated into 4 groups: sham group (n=12), I/R group (n=12), and I/R+SO2 groups (NaHSO3 and Na2SO3; 1:3 ratio; 5 and 10 µg/kg, respectively; for 3 days, n=12). Cerebral ischemia model was prepared by occlusion of both common carotid arteries for 20 min. Saline as a vehicle and SO2 donor at doses 5 µg/kg (intraperitoneally) were injected for 3 days after reperfusion. Four days after ischemia, the passive avoidance memory test was carried out in four groups, and after behavioral assessment, necrosis, apoptosis, and antioxidant enzyme analysis were carried out.
Results: SO2 treatment could significantly improve memory impairments in rats with cerebral ischemia/reperfusion (I/R) (P<0.05). An increase in both superoxide dismutase and glutathione and a reduction in malondialdehyde were reported in the SO2 group versus the ischemic group (P<0.05). Moreover, SO2 could significantly decrease necrotic and apoptotic cells in the CA1 region (P<0.01).
Conclusion: According to the findings, SO2 exerts significant neuroprotective effects on cerebral I/R due to its antioxidant activity.


Main Subjects

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