CircRNA_0109291 regulates cell growth and migration in oral squamous cell carcinoma and its clinical significance

Document Type : Original Article

Authors

1 Department of Oral Prosthodontics, the Affiliated Stomatological Hospital of Nanchang University, Jiangxi Provincial Key Laboratory of Oral Biomedicine, Nanchang 330006, China

2 Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Nanchang University, Nanchang 330006, China

Abstract

Objective(s): Circular RNAs (circRNAs), a new class of non-coding RNAs, have emerged as important regulators during tumorigenesis. However, the functions of circRNAs have not been completely clarified in the progression of cancers. In our study, a novel circRNA hsa_circ_0109291 was investigated in oral squamous cell carcinoma (OSCC) tissues and cell lines.
Materials and Methods: The expression profile of circRNAs in OSCC tumor tissues was performed by high-throughput sequencing. The CCK-8 wound healing and apoptosis assay were measured in OSCC cell lines after transfection with si-0109291 or si-NC.
Results: We discovered that hsa_circ_0109291 was significantly increased in OSCC tissues and cell lines compared with their corresponding control group. Knockdown of hsa_circ_0109291 inhibited proliferation and migration of OSCC cell lines in vitro. In addition, inhibition of hsa_circ_0109291 dramatically induced apoptosis of OSCC cells. We further found that high hsa_circ_0109291 levels in OSCC patients resulted in a poorer prognosis than in patients with low hsa_circ_0109291 levels.
Conclusion: These findings indicated that hsa_circ_0109291 correlated with the progression of OSCC and might be a new therapeutic target for the treatment of OSCC.

Keywords

Main Subjects

References

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Iranian Journal of Basic Medical Sciences
Volume 21, Issue 11
November 2018
Pages 1186-1191

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