Organ toxicity attenuation by nanomicelles containing curcuminoids: Comparing the protective effects on tissues oxidative damage induced by diazinon

Document Type : Original Article

Authors

1 Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran

2 Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran

3 Pharmacutical Science Research Center, Mazandaran University of Medical Sciences, Sari, Iran

4 Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s): Diazinon (DZ) is an organophosphate pesticide that induces oxidative damage in different organs. The aim of this study was to compare the effectiveness of nanomicelles containing curcuminoids (NCUR) and natural curcumin (CUR) in attenuating the oxidative damage induced by DZ in male rats.
Materials and Methods: After a single intraperitoneal (IP) injection of DZ (100 mg/kg), the rats were administered either CUR or NCUR (25 and 60 mg/kg, IP). Biomarkers of cell damage including, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), creatinine (Cr), urea, lactate dehydrogenase (LDH), creatine kinase-MB isoenzyme (CK-MB) and troponin I, were quantified in the serum. Lipid peroxidation (LPO) and glutathione (GSH) content in the liver, kidney, and heart tissues were determined.
Results: DZ administration increased the serum levels of ALT, AST, ALP, Cr, urea, LDH, CK-MB, and troponin I; however, the levels significantly (P<0.001) decreased in the CUR- and NCUR-treated groups compared to those in the DZ group. NCUR significantly decreased LPO (P<0.05) and increased GSH (P<0.05) in the heart, kidney, and liver tissues at all doses (especially, at 60 mg/kg) compared with CUR
Conclusion: Our findings suggest that NCUR treatment counters DZ-induced oxidative tissue damage to a greater extent than CUR.

Keywords

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References

1. Shiri M, Navaei-Nigjeh M, Baeeri M, Rahimifard M, Mahboudi H, Shahverdi AR, et al. Blockage of both the extrinsic and intrinsic pathways of diazinon-induced apoptosis in PaTu cells by magnesium oxide and selenium nanoparticles. Int J Nanotechnol Nanomed 2016; 11:6239.
2. Aggarwal V, Deng X, Tuli A, Goh KS. Diazinon—chemistry and environmental fate: A california perspective.  Rev Environ Contam Toxicol 2013; 223: 107-140.
3. Abdou H, El Mazoudy R. Oxidative damage, hyperlipidemia and histological alterations of cardiac and skeletal muscles induced by different doses of diazinon in female rats. J Hazard Mater 2010; 182:273-278.
4. Boussabbeh M, Salem IB, Hamdi M, Fradj SB, Abid-Essefi S, Bacha H. Diazinon, an organophosphate pesticide, induces oxidative stress and genotoxicity in cells deriving from large intestine. Environ Sci Pollut Res 2016; 23:2882-2889.
5. Sargazi Z, Nikravesh MR, Jalali M, Sadeghnia HR, Rahimi Anbarkeh F, Mohammadzadeh L. Diazinon-induced ovarian toxicity and protection by vitamins E. Iran J Toxicol 2014; 8:1130-1135.
6. Soltaninejad K, Abdollahi M. Current opinion on the science of organophosphate pesticides and toxic stress: a systematic review. Med Sci Monit 2009; 15:RA75-RA90.
7. Aluigi M, Guida C, Falugi C. Apoptosis as a specific biomarker of diazinon toxicity in NTera2-D1 cells. Chem Biol Interact 2010; 187:299-303.
8.Shah MD, Iqbal M. Diazinon-induced oxidative stress and renal dysfunction in rats. Food Chem Toxicol 2010; 48:3345-3353.
9.Jafari M, Salehi M, Ahmadi S, Asgari A, Abasnezhad M, Hajigholamali M. The role of oxidative stress in diazinon-induced tissues toxicity in Wistar and Norway rats. Toxicol Mech Methods 2012; 22:638-647.
10.Akturk O, Demirin H, Sutcu R, Yilmaz N, Koylu H, Altuntas I. The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat heart and ameliorating role of vitamin E and vitamin C. Cell Biol Toxicol 2006; 22:455-461.
11.Krieger R. Handbook of pesticide toxicology, two-volume set: principles and agents: Academic Press; 2001.
12.Ammon HP, Wahl MA. Pharmacology of Curcuma longa. Planta Medica 1991; 57:1-7.
13.Calabrese V, Bates TE, Mancuso C, Cornelius C, Ventimiglia B, Cambria MT, et al. Curcumin and the cellular stress response in free radical‐related diseases. Mol Nutr Food Res 2008; 52:1062-1073.
14.Soltani B, Ghaemi N, Sadeghizadeh M, Najafi F. Curcumin confers protection to irradiated THP-1 cells while its nanoformulation sensitizes these cells via apoptosis induction. Cell Biol Toxicol 2016; 32:543-561.
15.Pandeya N. Old wives’ tales: modern miracles—turmeric as traditional medicine in India. Trees Life J 2005; 1.
16.He Y, Yue Y, Zheng X, Zhang K, Chen S, Du Z. Curcumin, inflammation, and chronic diseases: how are they linked? Molecules 2015; 20:9183-9213.
17.Hatcher H, Planalp R, Cho J, Torti F, Torti S. Curcumin: from ancient medicine to current clinical trials. Cell Mol Life Sci 2008; 65:1631-1652.
18.Anand P, Kunnumakkara AB, Newman RA, Aggarwal BB. Bioavailability of curcumin: problems and promises. Mol Pharm 2007; 4:807-818.
19.Ghalandarlaki N, Alizadeh AM, Ashkani-Esfahani S. Nanotechnology-applied curcumin for different diseases therapy. Biomed Res Int 2014; 2014.
20.Shaikh J, Ankola D, Beniwal V, Singh D, Kumar MR. Nanoparticle encapsulation improves oral bioavailability of curcumin by at least 9-fold when compared to curcumin administered with piperine as absorption enhancer. Eur J Pharm Sci 2009; 37:223-230.
21.Prasad S, Tyagi AK, Aggarwal BB. Recent developments in delivery, bioavailability, absorption and metabolism of curcumin: the golden pigment from golden spice. Cancer Res Treat 2014; 46:2-18.
22.Messarah M, Amamra W, Boumendjel A, Barkat L, Bouasla I, Abdennour C, et al. Ameliorating effects of curcumin and vitamin E on diazinon-induced oxidative damage in rat liver and erythrocytes. Toxicol Ind Health 2013; 29:77-88.
23.Jaafari MR. Formulation and preparation of nanomicelles containing curcuminoids for oral use. Iranian Patent Number 83515 2014.
24. Rahimi HR, Mohammadpour AH, Dastani M, Jaafari MR, Abnous K, Mobarhan MG, et al. The effect of nano-curcumin on HbA1c, fasting blood glucose, and lipid profile in diabetic subjects: a randomized clinical trial. Avicenna J Phytomed 2016; 6:567-577.
25.Akinyemi AJ, Onyebueke N, Faboya OA, Onikanni SA, Fadaka A, Olayide I. Curcumin inhibits adenosine deaminase and arginase activities in cadmium-induced renal toxicity in rat kidney. J Food Drug Anal 2017; 25:438-446.
26. Sak ME, Soydinc HE, Sak S, Evsen MS, Alabalik U, Akdemir F, et al. The protective effect of curcumin on ischemia-reperfusion injury in rat ovary. Int J Surg  2013; 11:967-970.
27. Akinyemi AJ, Oboh G, Fadaka AO, Olatunji BP, Akomolafe S. Curcumin administration suppress acetylcholinesterase gene expression in cadmium treated rats. Neurotoxicology 2017; 62:75-79.
28. Katsumaro T, Tohru H. Diazinon concentrations and blood cholinesterase activities in rats exposed to diazinon. Toxicol Lett 1985; 25:7-10.
29. Izadi F, Jafari M, Bahdoran H, Asgari A, Divsalar A, Salehi M. The role of N-acetyl cysteine on reduction of diazinon-induced oxidative stress in rat liver and kidney. J Rafsanjan Univ Med Sci 2014; 12:895-906.
30. Fernandez F, Goudable C, Sie P, Ton‐That H, Durand D, Suc J, et al. Low haematocrit and prolonged bleeding time in uraemic patients: effect of red cell transfusions. Br J Haematol 1985; 59:139-148.
31. Moron MS, Depierre JW, Mannervik B. Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta Gen Subj 1979; 582:67-78.
32. Barzegar A. The role of electron-transfer and H-atom donation on the superb antioxidant activity and free radical reaction of curcumin. Food Chem 2012; 135:1369-1376.
33. Hoeijmakers JH. DNA damage, aging, and cancer. N Engl J Med 2009; 361:1475-1485.
34. Tiwari H, Rao MV. Curcumin supplementation protects from genotoxic effects of arsenic and fluoride. Food Chem Toxicol 2010; 48:1234-1238.
35. Notarbartolo M, Poma P, Perri D, Dusonchet L, Cervello M, D’Alessandro N. Antitumor effects of curcumin, alone or in combination with cisplatin or doxorubicin, on human hepatic cancer cells. Analysis of their possible relationship to changes in NF-kB activation levels and in IAP gene expression. Cancer Lett 2005; 224:53-65.
36. Yallapu MM, Jaggi M, Chauhan SC. Curcumin nanoformulations: a future nanomedicine for cancer. Drug Discov Today 2012; 17:71-80.
37. Yadav A, Lomash V, Samim M, Flora SJ. Curcumin encapsulated in chitosan nanoparticles: a novel strategy for the treatment of arsenic toxicity. Chem Biol Interact 2012; 199:49-61.
38. Srinivasan M, Prasad NR, Menon VP. Protective effect of curcumin on γ-radiation induced DNA damage and lipid peroxidation in cultured human lymphocytes. Mutat Res Genet Toxicol Environ Mutagen. 2006; 611:96-103.
39. Sonkaew P, Sane A, Suppakul P. Antioxidant activities of curcumin and ascorbyl dipalmitate nanoparticles and their activities after incorporation into cellulose-based packaging films. J Agric Food Chem 2012; 60:5388-5399.
40. Alp H, Aytekin I, Esen H, Basarali K, Kul S. Effects of caffeic acid phenethyl ester, ellagic acid, sulforaphane and curcumin on diazinon induced damage to the lungs, liver and kidneys in an acute toxicity rat model. Kafkas Univ Vet Fak Derg 2011; 17:927-933.
41. El-Shenawy NS, El-Salmy F, Al-Eisa RA, El-Ahmary B. Amelioratory effect of vitamin E on organophosphorus insecticide diazinon-induced oxidative stress in mice liver. Pestic Biochem Physiol 2010; 96:101-107.
42. Ozer J, Ratner M, Shaw M, Bailey W, Schomaker S. The current state of serum biomarkers of hepatotoxicity. Toxicol 2008; 245:194-205.
43. Oruç EÖ, Usta D. Evaluation of oxidative stress responses and neurotoxicity potential of diazinon in different tissues of Cyprinus carpio. Environ Toxicol Pharmacol 2007; 23:48-55.
44. Isik I, Celik I. Acute effects of methyl parathion and diazinon as inducers for oxidative stress on certain biomarkers in various tissues of rainbowtrout (Oncorhynchus mykiss). Pestic Biochem Physiol 2008; 92:38-42.
45. Priscilla DH, Prince PSM. Cardioprotective effect of gallic acid on cardiac troponin-T, cardiac marker enzymes, lipid peroxidation products and antioxidants in experimentally induced myocardial infarction in Wistar rats. Chem Biol Interact 2009; 179:118-124.
46. Hariri AT, Moallem SA, Mahmoudi M, Memar B, Hosseinzadeh H. Sub-acute effects of diazinon on biochemical indices and specific biomarkers in rats: protective effects of crocin and safranal. Food Chem Toxicol 2010; 48:2803-2808.
47. Kose A, Gunay N, Yildirim C, Tarakcioglu M, Sari I, Demiryurek AT. Cardiac damage in acute organophosphate poisoning in rats: Effects of atropine and pralidoxime. Am J Emerg Med 2009; 27:169-175.
48. Tirkey N, Kaur G, Vij G, Chopra K. Curcumin, a diferuloylmethane, attenuates cyclosporine-induced renal dysfunction and oxidative stress in rat kidneys. BMC Pharmacol 2005; 5:1-10.
49. Singh RP, Sharad S, Kapur S. Free radicals and oxidative stress in neurodegenerative diseases: relevance of dietary antioxidants. J Indian Acad Clin Med 2004; 5:218-225.
50. Kumar A, Dogra S, Prakash A. Protective effect of curcumin (Curcuma longa), against aluminum toxicity: Possible behavioral and biochemical alterations in rats. Behav Brain Res 2009; 205:384-390.
51. Rao M. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997; 49:105-107.
52. Somparn P, Phisalaphong C, Nakornchai S, Unchern S, Morales NP. Comparative antioxidant activities of curcumin and its dimethoxy and hydrogenated derivatives. Biol Pharm Bull 2007; 30:74-78.
53. Ramawat KG, Dass S, Mathur M. Herbal drugs: ethnomedicine to modern medicine: Springer; 2009.
Iranian Journal of Basic Medical Sciences
Volume 22, Issue 1
January 2019
Pages 17-24

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