Comparative volatile composition, antioxidant and cytotoxic evaluation of the essential oil of Zhumeria majdae from south of Iran

Document Type : Original Article


1 Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran


Objective(s): The purpose of this study was to evaluate variations in yields, volatile composition and biological activities of essential oils (EOs) obtained from the aerial parts of Zhumeria majdae collected from five localities of the south of Iran.
Materials and Methods: The EOs were analyzed using gas chromatography and gas chromatography-mass spectrometry techniques. The antioxidant activity of the EOs was tested using DPPH and β-carotene/linoleic acid assays. In vitro cytotoxicity was tested against two cancer cell lines (A375 and MCF7) using MTT assay.
Results: The oils yield varied from 6.3% (S2) to 10.2% (V/W) (S4). All of five investigated EOs samples presented three major compounds: linalool (24.4-34.6%), camphor (26.1-34.7%) and trans-linalool oxide (7.6-28.6%). Although the main constituents were common, their percentages were different. Among samples, S1 had a better antioxidant activity in both DPPH and β-carotene/linoleic acid methods (IC50= 8.01 and 11.77 mg/ml, respectively). In vitro cytotoxicity against two cancer cell lines of human melanoma cell line (A375) and breast cancer cell line (MCF7), showed a moderate cytotoxicity of S3 against A375 cells with IC50 value of 624 μg/ml.
Conclusion: Tangezagh (S4) plant materials revealed the highest level of oil yield as the region is recommended for collecting the plant samples.Taken together, despite the weak antioxidant and moderate cytotoxic activities of tested EOs, this study suggested a proper potential for possible use of the EOs of Z. majdae for pharmaceutical and perfume industries.


Main Subjects

1. Emami SA, Aghazari F. Les Phanerogames Endemiquesde la Flore d’Iran.  2011:407.
2. Moein S, Moein MR. Relationship between antioxidant properties and phenolics in Zhumeria majdae. J Med Plants Res 2010; 4:517-521.
3. Goodarzi  S, Hadjiakhoondi  A, Yassa N, Khanavi M, Tofighi Z. Essential oils chemical composition, antioxidant activities and total phenols of Astrodaucus persicus. Iran J Basic Med Sci 2016; 19:159-165
4. Majrouhi A. Chemical composition of the leaf essential oil of Zhumeria majdae growing in South Iran. Chem Nat Compd 2009; 45:429-430.
5. Rustaiyan A, Samadizadeh M, Habibi Z, Jakupovic J. Two diterpenes with rearranged abietane skeletons from Zhumeria majdae. Phytochemistry 1995; 39: 163-165.
6. Izaddoost M, Rustaiyan A. Phytochemical study on Zhumeria majdae. Fitoterapia 1983; 45:70-76.
7. Moein MR, Pawar RS, Khan SI, Tekwani BL, Khan IA. Antileishmanial, antiplasmodial and cytotoxic activities of 12, 16‐dideoxy aegyptinone B from Zhumeria majdae Rech. f. & Wendelbo. Phytother Res 2008; 22:283-285.
8. Sharififar F, Khazaeli P, Alli N, Talebian E, Zarehshahi R, Amiri S. Study of antinociceptive and anti-inflammatory activities of certain Iranian medicinal plants. J Intercult Ethnopharmacol 2012; 1: 19-24.
9. Hosseinzadeh H, Ramezani M, Fadishei M, Mahmoudi M. Antinociceptive, anti-inflammatory and acute toxicity effects of Zhumeria majdae extracts in mice and rats. Phytomedicine 2002; 9:135-141.
10. Mandegary A, Sharififar F, Abdar M, Arab-Nozari M. Anticonvulsant activity and toxicity of essential oil and methanolic extract of Zhumeria majdae rech, a unique Iranian plant in mice. Neurochemical Res 2012; 37:2725-2730.
11. Soltani Poor M, Rezaee M, Moradshahi A. Study on antimicrobial effects of essential oil of Zhumeria majdae Rech. f. & Wendelbo. Iran J Med Arom Plants Res 2004; 20:277-289.
12. Arman M, Yousefzadi M, Ebrahimi SN. Antimicrobial Activity and Composition of the Essential Oil from Zhumeria majdae Rech. f. & Wendelbo. J Essent Oil Bear Pl 2009; 12:630-634.
13. Ebadollahi A, Khosravi R, Sendi JJ, Mahboubi M, Kosari AA. Chemical composition of essential oil from Zhumeria majdae Rech. F. & Wendelbo and its bioactivities against Tribolium castaneum Herbst (Tenebrionidae) larvae. J Essent Oil Bear Pl 2014; 17:824-831.
14. Rustaiyan A, Sigari H, Bamoniri A, Weyerstahl P. Constituents of the essential oil of Zhumeria majdae Rech. Flavour Frag J 1992; 7: 273-274.
15. Abbaszadeh B, Teymoori M, Pouyanfar M, Rezaei M, Mafakheri S. Growth and essential oil of Mentha longifolia L. var. amphilema from different ecological conditions. Ann Biol Res 2013; 4:85-90.
16.Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry: Allured Publishing Corporation; 2007.
17. Shakeri A, Khakdan F, Soheili V, Sahebkar A, Shaddel R, Asili J. Volatile composition, antimicrobial, cytotoxic and antioxidant evaluation of the essential oil from Nepeta sintenisii Bornm. Ind Crops Prod 2016; 84:224-229.
18. Mensor LL, Menezes FS, Leitão GG, Reis AS, Santos TCd, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001; 15:127-130.
19. Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food chem 2004; 85:633-640.
20. Smitha G, Rana VS. Variations in essential oil yield, geraniol and geranyl acetate contents in palmarosa (Cymbopogon martinii, Roxb. Wats. var. motia) influenced by inflorescence development. Ind Crop Prod 2015; 66:150-160.
21. Boira H, Blanquer A. Environmental factors affecting chemical variability of essential oils in Thymus piperella L. Biochem syst ecol 1998; 26:811-822.
22. Sharififar F, Mozaffarian V, Moshafi M, Dehghan-Nudeh G, Parandeh-Rezvani J, Mahdavi Z. Chemical composition and biological activities of Zhumeria majdae Resh. F. & wendelbo. Jundushapur J Nat Pharm Prod 2007; 3: 8-18.
23. Moein S, Moein MR. Relationship between antioxidant properties and phenolics in Zhumeria majdae. J Med Plants Res 2010; 4:517-521.
24. Kavoosi G, Rowshan V. Chemical composition, antioxidant and antimicrobial activities of essential oil obtained from Ferula assa-foetida oleo-gum-resin: effect of collection time. Food chem 2013; 138:2180-2187.
25. Zaouali Y, Bouzaine T, Boussaid M. Essential oils composition in two Rosmarinus officinalis L. varieties and incidence for antimicrobial and antioxidant activities. Food Chem Toxicol 2010; 48:3144-3152.
26. Loizzo MR, Tundis R, Menichini F, Saab AM, Statti GA, Menichini F. Cytotoxic activity of essential oils from Labiatae and Lauraceae families against in vitro human tumor models. Anticancer Res 2007; 27:3293-3299.
27. Chang M-Y, Shen Y-L. Linalool exhibits cytotoxic effects by activating antitumor immunity. Molecules 2014; 19:6694-6706.
28. Sibanda S, Chigwada G, Poole M, Gwebu ET, Noletto JA, Schmidt JM, et al. Composition and bioactivity of the leaf essential oil of Heteropyxis dehniae from Zimbabwe. J Ethnopharmacol 2004; 92:107-111.
29. Silva-Filho SE, de Souza Silva-Comar FM, Wiirzler LAM, do Pinho RJ, Grespan R, Bersani-Amado CA, et al. Effect of camphor on the behavior of leukocytes in vitro and in vivo in acute inflammatory response. Trop J Pharml Res 2015; 13:2031-2037.
30. Cherneva E, Pavlovic V, Smelcerovic A, Yancheva D. The effect of camphor and borneol on rat thymocyte viability and oxidative stress. Molecules 2012; 17:10258-10266.