Evaluation of Immunological Parameters in Purified Protein Derivative Positive Tuberculin Workers

Document Type : Original Article


1 Department of Microbiology, Faculty of Science, Islamic Azad University, Karaj Branch, Karaj, Iran

2 Razi Vaccine and Serum Research Institute, Karaj, Iran

3 Department of Allergy and Clinical Immunology, Rasoul Akram Hospital, Tehran University of Medical Sciences, Tehran, Iran



According to the occupationally risk of infection in staff workers who have direct contact with mycobacterium species, we investigated their immunological parameters and compared with healthy purified protein derivative (PPD) negative volunteers.
Materials and Methods
: We investigated 20 PPD positive volunteers working at Tuberculin Unit of Razi Vaccine and Serum Research Institute and PPD negative healthy controls with no exposure or history of active tuberculosis. The percentages of circulating lymphocyte subpopulations were detected by flowcytometry. IL-4 and IFN-γ production levels were measured by ELISA in supernatants of PPD-stimulated peripheral blood mononuclear cells (PBMCs) culture.
: Tuberculin workers showed an increase in IFN-γ level and significant decrease of CD4+ T cells percentage and CD4/CD8 ratio compared to PPD negative normal individuals. However the IL-4 production and percentage of other lymphocyte population has been unchanged.
These observations suggest that the immunological parameters of tuberculin workers with PPD positive reaction, who are occupationally exposed to mycobacterium antigens, could be changed. Future studies will be directed towards cytokine networking and regulatory lymphocytes, which will help us validate the significant data presented in this study.


Swaminathan S. Tuberculosis, the only infection disease to be declared a global emergency by the WHO, is a major cause of death in adults and children worldwide. J Pediatr 2000; 67:S1-S2
2. Texeria CH, Abramo C, Munk ME. Immunological diagnosis of tuberculosis, problems and strategies for success. J Bras Pneumol 2007; 33:323-334.
3. Lange C, Mori T. Advances in the diagnosis of tuberculosis. Respirology 2010; 15:220-240.
4. Cho SN. Current issues on molecular and immunological diagnosis of tuberculosis. Yonsei Med J 2007; 48:347-359.
5. Smith I. Mycobacterium tuberculosis pathogenesis and molecular determinants of virulence. Clin Microbial Rev 2003; 16:463-496.
6. Dietrich J, Doherty TM. Interaction of
Mycobacterium tuberculosis
with the host: consequence For Vaccine development. APMIS. 2009;117(5-6):440-57
7. Schluger NW, Rom WN. The host immune response to tuberculosis. Am J Respir Crit Care Med 1998; 157:679-691.
8. Caccamo N, Guggino G, Meraviglia S, Gelsomino G, Di Carlo P, Titone L,
et al.
Analysis of Mycobacterium tuberculosis-specific CD8 T-cells in patients with active tuberculosis and in individuals with latent infection. PLoS One 2009; 4:e 5528.
9. Uppal SS, Tewari SC,Verma S, Dhot PS. Comparison of CD4
+ and CD8+
Lymphocyte counts in HIV-Negative Pulomonary TB patients with those in normal blood donors and the effect of antitubercular treatment: Hospital-Based flow cytometric study. Cytometry Clin Cytometry 2004; 61B:20-26.
10. Jashua SM. Wood Worth and Samuel M.Behar
؟؟. Mycobacterium tuberculosis specific CD8+ T Cells and Their role in immunity.
Crit Rev Immunol 2006; 26:317-352.
11. Vilcek J, Kilon A, Henriksen-Destefano D, Zemts, A , Davidson DM, Davidson M,
et al.
Defective gamma-interferon production in peripheral blood leukocytes of patients with acute tuberculosis. J Clin Immunol 198 6; 6:146-51.
12. Sodhi A, Gong J, Silva C, Gian D, Barnes PF. Clinical Correlates of interferon gamma production in patients with tuberculosis. Clin Infect Dis 1997; 25:617-620.
13. Raje A. Immunology of tuberculosis. Indian J Med Res 2004; 120:213-232.