Thiamine reduced metabolic syndrome symptoms in rats via down-regulation of hepatic nuclear factor-kβ and induction activity of glyoxalase-I

Document Type : Original Article

Authors

1 Department of Clinical Biochemistry, Ardabil University of Medical Sciences, Ardabil, Iran

2 Department of Genetics and Pathology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

3 Research Laboratory for Embryology and Stem Cells, Department of Anatomical Sciences, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Abstract

Objective(s): Metabolic syndrome (MS) is a cause of death worldwide. The hepatic nuclear factor- NF-kβ (NF-kβ) is the cardinal player of hepatic homeostasis, insulin sensitivity, and lipid metabolism. Thus, we investigated the effect of thiamine on hepatic gene expression of NF-kβ and its levels of activators in MS rats.
Materials and Methods: Male Wistar rats were randomly divided into 4 equal groups (ten rats in each group): normal, MS, and two alike groups under thiamine treatment. MS was induced in rats with a high sucrose solution (40 % in drinking water) for 4 months. Treated groups of rats received 0.18 % of thiamine daily in drinking water. Hematoxylin-Eosin stains were employed to determine the histopathological changes of the liver. Metabolic profile, glycation products, oxidative stress, inflammatory markers, the activity of glyoxalase-I, as well as NF-kβ hepatic expression of all rat groups, were determined.
Results: Acute hepatitis was not observed in the livers of the thiamine treated MS rats. Besides, the treatment showed an advantageous effect on glucose, lipid metabolism, and body weight via down-regulation of hepatic NF-kβ and induction of glyoxalase system activity. Furthermore, the treatment decreased diverse glycation, oxidative stress, and inflammatory markers (P>0.001).
Conclusion: Thiamine decreased body weight and improved metabolism and activity of glyoxalase-I in MS rats with anti-glycation, antioxidant, and anti-inflammatory activities. Further, the treatment had a hepato-protective effect via reduction of NF-kβ signaling.

Keywords

References

1. Hess PL, Al-Khalidi HR, Friedman DJ, Mulder H, Kucharska-Newton A, Rosamond WR, et al. The metabolic syndrome and risk of sudden cardiac death: The atherosclerosis risk in communities study. J Am Heart Assoc 2017;6: 6103-6114.
2. Spahis S, Borys JM, Levy E. Metabolic syndrome as a multifaceted risk factor for oxidative stress. Antioxid Redox Signal 2017; 26:445-461.
3.  Mazloomzadeh S, Karami Zarandi F, Shoghli A, Dinmohammadi H. Metabolic syndrome, its components and mortality: A population-based study. Med J Islam Repub Iran 2019; 33:11-16.
4. Gehrke N, Schattenberg JM. Metabolic inflammation-a role for hepatic inflammatory pathways as drivers of comorbidities in nonalcoholic fatty liver disease? Gastroenterology 2020; 158:1929-1947.
5. Petersen MC SG. Mechanisms of insulin action and insulin resistance. Physiol Rev 2018; 98:2133-2223.
6. Piuri G, Basello K, Rossi G, Soldavini CM, Duiella S, Privitera G, et al. Methylglyoxal, glycated albumin, paf, and tnf-α: possible inflammatory and metabolic biomarkers for management of gestational diabetes. Nutrients 2020; 12-27.
7. Wen  Jin BC, Pingping Li, FangHua, Xiaoxi Lv, Jichao Zhou, Zhuowei Hu Xiaowei Zhang. 1,25-Dihydroxyvitamin D3 protects obese rats from metabolic syndrome via promoting regulatory Tcell-mediated resolution of inflammation. Acta Pharmaceutica Sinica B  2018; 8:178–187.
8. Jayarathne S, Koboziev I, Park OH, Oldewage-Theron W, Shen CL, Moustaid-Moussa N. Anti-inflammatory and anti-obesity properties of food bioactive components: effects on adipose tissue. Prev Nutr Food Sci 2017; 22:251-262.
9. Okura T, Ueta E, Nakamura R, Fujioka Y, Sumi K, Matsumoto K, et al. High serum advanced glycation end products are associated with decreased insulin secretion in patients with type 2 diabetes: a brief report. J Diabetes Res 2017; 2017:5139750.
10. Mahdavifard S, Nakhjavani M. Thiamine pyrophosphate improved vascular complications of diabetes in rats with type 2 diabetes by reducing glycation, oxidative stress, and inflammation markers. Med J Islam Repub Iran 2020; 34:331-336.
11. Mahdavifard S, Nakhjavani M. Effect of Pyridoxal phosphate on atherosclerosis and nephropathy progression in atherosclerotic rats. J Adv Med Biomed Res 2021; 29:21-27.
12. Uribarri JC, Woodward W, Tripp M, Goldberg E,  Pyzik L,  Yee R,  et al. Elevated serum advanced glycation endproducts in obese indicate risk for the metabolic syndrome: A link between healthy and unhealthy obesity? J Clin Endocrinol MeTable 2015; 100:1957-1966.
13. R I. RAGE and glyoxalase in kidney disease. Glycoconj J 2016; 33:619-626.
14. Mahdavifard S, Nakhjavani M. Effect of linalool on the activity of glyoxalase-i and diverse glycation products in rats with type 2 diabetes. J Maz Uni Med Sci 2020; 30:24-33.
15. Nigro C, Leone A, Raciti GA, Longo M, Mirra P, Formisano P, et al. Methylglyoxal-glyoxalase 1 balance: the root of vascular damage. Int J Mol Sci 2017; 18:188-202.
16. Kerns JC, Arundel C, Chawla LS. Thiamin deficiency in people with obesity. Advances in nutrition 2015; 6:147-153.
17. Alaei-Shahmiri F, Soares MJ, Zhao Y, Sherriff J. The impact of thiamine supplementation on blood pressure, serum lipids and C-reactive protein in individuals with hyperglycemia: A randomised, double-blind cross-over trial. Diabetes Metab Syndr 2015; 9:213-217.
18. Tanaka T, Kono T, Terasaki F, Yasui K, Soyama A, Otsuka K, et al. Thiamine prevents obesity and obesity-associated metabolic disorders in OLETF rats. J Nutr Sci Vitaminol 2010; 56:335-346.
19. Souza Cruz EM, Bitencourt de Morais JM, Dalto da Rosa CV, da Silva Simões M, Comar JF, de Almeida Chuffa LG, et al. Long-term sucrose solution consumption causes metabolic alterations and affects hepatic oxidative stress in Wistar rats. Biol Open 2020; 9:1-10
20. Rabbani N, Alam SS, Riaz S, Larkin J, Akhtar MW, Shafi T, et al. High-dose thiamine therapy for patients with type 2 diabetes and microalbuminuria: a randomised, double-blind placebo-controlled pilot study. Diabetologia 2009; 52:208-212.
21. Lv ZH, Ma P, Luo W, Xiong H, Han L, Li SW, et al. Association between serum free fatty acid levels and possible related factors in patients with type 2 diabetes mellitus and acute myocardial infarction. BMC Cardiovasc Disord 2014; 14:159-66.
22. Matthews DR HJ, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: Insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia 1985; 28:412-419.
23. Song YS, Hwang YC, Ahn HY, Park CY. Comparison of the usefulness of the updated homeostasis model assessment (HOMA2) with the original HOMA1 in the prediction of type 2 diabetes mellitus in koreans. Diabetes Metab J 2016; 40:318-325.
24. Xu YJ, Wu XQ, Liu W, Lin XH, Chen JW, He R. A convenient assay of glycoserum by nitroblue tetrazolium with iodoacetamide. Clinica Chimica Acta 2002; 325:127-131.
25. Cohen MP, Shea EA, Wu V-Y. Inhibiting LDL glycation ameliorates increased cholesteryl ester synthesis in macrophages and hypercholesterolemia and aortic lipid peroxidation in streptozotocin diabetic rats. Metabolism 2010; 59:658-663.
26. Pappalardo M, Pappalardo L, Brooks P. Rapid and Reliable HPLC Method for the Simultaneous Determination of Dihydroxyacetone, Methylglyoxal and 5-Hydroxymethylfurfural in Leptospermum Honeys. PLoS One 2016; 11:1-9.
27. Kalousova M, Skrha J, Zima T. Advanced glycation end-products and advanced oxidation protein products in patients with diabetes mellitus. Physiol Res 2002; 51:597-604.
28. Mahdavifard S, Bathaie S, Nakhjavani M, Heidarzadeh H. L-cysteine is a potent inhibitor of protein glycation on both albumin and LDL, and prevents the diabetic complications in diabetic–atherosclerotic rat. Food Research International 2014; 62:909-916.
29. Witko-Sarsat V, Friedlander M, Capeillère-Blandin C, Nguyen-Khoa T, Nguyen AT, Zingraff J, et al. Advanced oxidation protein products as a novel marker of oxidative stress in uremia. Kidney Int. 1996; 49:1304-1313.
30. Ahotupa M, Marniemi J, Lehtimäki T, Talvinen K, Raitakari OT, Vasankari T, et al. Baseline diene conjugation in LDL lipids as a direct measure of in vivo LDL oxidation. Clin Biochem 1998; 31:257-261.
31. Esterbauer H, Gebicki J, Puhl H, Jürgens G. The role of lipid peroxidation and anti-oxidants in oxidative modification of LDL. Free Radic Biol and Med 1992; 13:341–390.
32. Begic A, Djuric A, Gobeljic B, Stevanovic I, Lukic V, Stanojevic I, et al. The simple isocratic HPLC—UV method for the simultaneous determination of reduced and oxidized glutathione in animal tissue. Acta Chromatographica 2017; 29:67-84.
33. Ceron JJ, Tecles F, Tvarijonaviciute A. Serum paraoxonase 1 (PON1) measurement: an update. BMC Vet Res 2014; 10:74-85.
34. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121-129.
35. Mazani M, Rezagholizadeh L, Shamsi S, Mahdavifard S, Ojarudi M, Salimnejad R, et al. Protection of CCl(4)-induced hepatic and renal damage by linalool. Drug Chem Toxicol 2020:1-9.
36. Kavita S, Neerja, R., Khushboo, G. & Saurabh, S. Probable benefits of green tea with genetic implications J Oral Maxillofac Pathol 2017; 21:107-114.
37. Gao D MM, Ding C, Fok M, Steele T, Ford C, et al. Interleukin-1beta mediates macrophage-induced impairment of insulin signaling in human primary adipocytes. Am J Physiol Endocrinol Metab 2014; 307:289-304.
38. Bing C. Is interleukin-1β a culprit in macrophage-adipocyte crosstalk in obesity? Adipocyte 2015; 4:149-152.
39. Mahdavifard S, Nakhjavani M. Effect of glutamine on oxidative stress, inflammatory, and glycation markers, and the activity of glyoxalase system in diabetic rats with atherosclerosis. J Mazandaran Univ Med Sci 2019; 28:33-42.
40. Zhang H, Peng AL, Zhao FF, Yu LH, Wang MZ, Osorio JS, et al. Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis. J Dairy Sci 2020; 103:1931-1943.
41. Maguire D, Talwar D, Shiels PG, McMillan D. The role of thiamine dependent enzymes in obesity and obesity related chronic disease states: A systematic review. Clin Nutr ESPEN 2018; 25:8-17.
42. Fernandes LMP, Bezerra FR, Monteiro MC, Silva ML, de Oliveira FR, Lima RR, et al. Thiamine deficiency, oxidative metabolic pathways and ethanol-induced neurotoxicity: how poor nutrition contributes to the alcoholic syndrome, as Marchiafava-Bignami disease. Eur J Clin Nutr 2017; 71:580-586.
43. Page GL, Laight D, Cummings MH. Thiamine deficiency in diabetes mellitus and the impact of thiamine replacement on glucose metabolism and vascular disease. Int J Clin Pract 2011; 65:684-690.
44. Page G, Laight D, Cummings M. Thiamine deficiency in diabetes mellitus and the impact of thiamine replacement on glucose metabolism and vascular disease. Int J Clin Prac 2011; 65:684-690.
45. vinh quoc Luong K, Nguyen LTH. The impact of thiamine treatment in the diabetes mellitus. J Clin Med Res 2012; 4:153-160.
46. Verdile G, Keane KN, Cruzat VF, Medic S, Sabale M, Rowles J, et al. Inflammation and oxidative stress: the molecular connectivity between insulin resistance, obesity, and Alzheimer’s disease. Mediators Inflamm 2015; 2015:1-17.
47. Rodrigues T, Matafome P, Sereno J, Almeida J, Castelhano J, Gamas L, et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 2017; 7:1698-1711
48. Frizell EL, SL, Abraham V, Ozaki I, Eghbali M, Sage EH, Zern MA. Expression of SPARC in normal and fibrotic livers. Hepatology 1995; 21: 847–854.
49. Waheed P, Naveed AK, Ahmed T. Thiamine deficiency and its correlation with dyslipidaemia in diabetics with microalbuminuria. J Pak Med Assoc. 2013; 63:340-345.
50. Thornalley PJ. The potential role of thiamine (vitamin B1) in diabetic complications. Curr Diabetes Rev 2005; 1:287–298.
51. Cieslak M WA, Cieslak M. Role of pro-inflammatory cytokines of pancreatic islets and prospects of elaboration of new methods for the diabetes treatment. Acta Biochim Pol 2015; 62:15-21.
52. Rumberger JM WT, Hering MA, Marshall S. Role of hexosamine biosyn-thesis in glucose-mediated up-regulation of lipogenic enzyme mRNA levels: effects of glucose, glutamine, and glucosamine on glycerophosphate dehy-drogenase, fatty acid synthase, and acetyl-CoA carboxylase mRNA levels. J Biol Chem 2003; 278:28547–28552.
53. Sarandol E, Tas S, Serdar Z, Dirican M. Effects of thiamine treatment on oxidative stress in experimental diabetes. Bratisl Lek Listy 2020; 121:235-241.
54. Kattoor AJ, Kanuri SH, Mehta JL. Role of Ox-LDL and LOX-1 in atherogenesis. Curr Med Chem 2019; 26:1693-1700.
55. Harrison DG, Gongora MC. Oxidative stress and hypertension. Med Clin North Am 2009;93: 621-635.

Iranian Journal of Basic Medical Sciences
Volume 24, Issue 3
March 2021
Pages 293-299

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