Antibacterial Activity of Elephant Garlic and Its Effect against U2OS Human Osteosarcoma Cells

Document Type : Original Article

Authors

1 Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China

2 Jain-Wu Ren, College of Biological Sciences and Biotechnology, Beijing Forestry University Beijing 10083, China

Abstract

 




Objective(s):
The present study was designed to investigate the antibacterial function and pharmacological effect of elephant garlic (Allium ampeloprasum var. ampeloprasum) on U2OS human osteosarcoma cells.
 
Materials and Methods:
Seven kinds of bacteria were reconstituted, inoculated and tested in this research to evaluate elephant garlic antibacterial activity. By the means of FACS analysis, cell proliferation assay, confocal laser scanning microscopy and Transwell migration assays, the effect of elephant garlic against U2OS human osteosarcoma cells was unveiled.
Rerults:
The antimicrobial activity of elephant garlic was stronger than ampicillin when used against Escherichia coli, Bacillus subtilis, Bacillus thuringiensis, Staphylococcus actinomycetes, and gray actinomycetes. Even at a very low concentration (12.5%), elephant garlic still had an antibacterial effect on common bacteria E. coli and S. aureus. The G0/G1 ratio of elephant garlic treated group cells increased while S phase decreased. Elephant garlic extract inhibited the growth of human osteosarcoma cells, U2OS, through preventing the transition from G1 phase to S phase. It reduced osteosarcoma cell, U2OS, invasion ability and significantly increased the proportion of apoptosis. It significantly affected the cytoskeleton generation.
Conclusion:
Elephant garlic exhibits antibacterial property and has an inhibitory effect on osteosarcoma cells (U2OS) proliferation and cell activity, suggesting the mechanism of its anticancer effects on U2OS human osteosarcoma cells.

Keywords

References

 
1. Gantait S, Mandal N, Das PK. Field evaluation of micropropagated vs. conventionally propagated elephant garlic[J]. J Agri Technol 2010; 7: 97-103.
2. Ahmadabad HN, Zuhair MH, Safari E, Bozorgmehr M , Moazzeni SM. Evaluation of the effect of the 47
kDa protein isolated from aged garlic extract on dendritic cells. Iran J Basic Med Sci 2012; 15:745-751.
3. Antony ML, Singh SV. Molecular mechanisms and targets of cancer chemoprevention by garlic-derived bioactive compound diallyl trisulfide[J]. Indian J Exp Biol 2011; 49:805.
4. Rounds L, Havens C M, Feinstein Y.?, et al. Plant extracts, spices, and essential oils inactivate Escherichia coli O157: H7 and reduce formation of potentially carcinogenic heterocyclic amines in cooked beef patties[J]. J Agric food Chem 2012; 60:3792-3799.
5. Delshad AA, Heshmati M. Garlic extract can induce apoptotic cell death in the human colon adenocarcinoma HT29 cell line. Iran J Pathol 2010; 5:126-131.
6. Slusarenko AJ, Patel A, Portz D. Control of plant diseases by natural products: Allicin from garlic as a case study[M]//Sustainable disease management in a European context. Netherlands: Springer; 2008.p. 313-322.
7. Block E, Naganathan S, Putman D, Zhao SH. Allium chemistry: HPLC analysis of thiosulfinates from onion, garlic, wild garlic (ransoms), leek, scallion, shallot, elephant (great-headed) garlic, chive, and Chinese chive. Uniquely high allyl to methyl ratios in some garlic samples. J Agric Food Chem 1992; 40:2418-2430.
8. 8 Rattanachaikunsopon P, Phumkhachorn P. Antimicrobial activity of elephant garlic oil against Vibrio cholerae in vitro and in a food model[J]. Biosci Biotechnol Biochem 2009; 73:1623-1627.
9. Sata N, MatSUNAGA S, FUSETANI N. New Antifungal and Cytotoxic Steroidal Saponins from the Bulbs of an Elephant Garlic Mutant[J]. Biosc Biotechnol Biochem 1998; 62:1904-1911.
10. Pai MH, Kuo YH, Chiang EPI. S-Allylcysteine inhibits tumour progression and the epithelial–mesenchymal transition in a mouse xenograft model of oral cancer[J]. Br J Nutr 2012; 108:28.
11. Karaman I, Sahin F, Gullule M. Antimicrobial activity of aqueous and methanol extracts of Juniperus Oxycedrus L. J Ethano Pharmacol 2003; 85:5-231.
12. Masika PJ, Afolayan AJ. Antimicrobial activity of some plants used for the treatment of livestock disease in the Eastern Cape. South Africa. J Ethanopharmacol 2002; 83:34-129.
13. Bathaie SZ, Miri H, Mohagheghi MA. Saffron aqueous extract inhibits the chemically-induced gastric cancer progression in the wistar albino rat[J]. Iran J Basic Med Sci 2013; 16: 27.
14. Sadeghi Aliabadi H, Emami Ahmad SB, Jafarian A. In vitro cytotoxicity of two subspecies of Juniperus excelsa on cancer cells[J]. Iran J Basic Med Sci 2009; 11:250-253.
15. Razavi SM, Zarrini MG, Ghasemi G. Bioactivity of Malva sylvestris L., a medicinal plant from Iran[J]. Iran J Basic Med Sci 2011.
16. Zhang P, Yang Y, Zweidler-McKay PA. Critical role of notch signaling in osteosarcoma invasion and metastasis[J]. Clin Cancer Res 2008; 14:2962-2969.
17. Volk T, Geiger B, Raz A. Motility and adhesive properties of high-and low-metastatic murine neoplastic cells [J]. Cancer Res 1984; 44:811-824.
Elephant Garlic against U2OS Human Osteosarcoma Cells Huang et al
Iran J Basic Med Sci, Vol. 16, No. 10, Oct 2013
 
1094
　
18. Sijin L. The ROCK signaling and breast cancer metastasis. Mol Biol Rep 2011; 38:1363–1366.
19. Prager‐Khoutorsky M, Goncharov I, Rabinkov A. Allicin inhibits cell polarization, migration and division via its direct effect on microtubules[J]. Cell motility and the cytoskeleton 2007; 64:321-337.
20. Milner JA. A historical perspective on garlic and cancer[J]. J Nutr 2001; 131:1027S-1031S.
21. El Astal Z. The inhibitory action of aqueous garlic extract on the growth of certain pathogenic bacteria[J]. Eur Food Res Technol 2004; 218:460-464.
22. Rattanachaikunsopon P, Phumkhachorn P. Diallyl sulfide content and antimicrobial activity against food-borne pathogenic bacteria of chives (Allium
schoenoprasum)[J]. Biosci Biotechnol Biochem 2008; 72:2987-2991.
23. Wargovich MJ. Diallyl sulfide, a flavor component of garlic (Allium sativum), inhibits dimethyihydrazine-induced colon cancer [J]. Carcinogenesis 1987; 8:487-489.
24. Stan SD, Kar S, Stoner GD. Bioactive food components and cancer risk reduction [J]. J Cell Biochem 2008; 104:339-356.
25. Sahai E, Marshall CJ. Differing modes of tumour cell invasion have distinct requirements for Rho/ROCK signalling and extracellular proteolysis[J]. Nat Cell Biol 2003, 5:711-719.
Iranian Journal of Basic Medical Sciences
Volume 16, Issue 10 - Serial Number 10
October 2013
Pages 1088-1094

Files

Share

How to cite

Statistics