Potential protective effect of astaxanthin on ovary ischemia-reperfusion injury

Document Type : Original Article


1 Kafkas University, Faculty of Medicine, Department of Histology and Embryology, Kars, Turkey

2 Erzincan Binali Yildirim University, Faculty of Medicine, Department of Histology and Embryology, Erzincan, Turkey

3 Ataturk University, Faculty of Medicine, Department of Histology and Embryology, Erzurum, Turkey

4 Ataturk University, Faculty of Pharmacy, Department of Pharmacology, Erzurum, Turkey

5 Alanya Alaaddin Keykubat University, Faculty of Medicine, Department of Histology and Embryology, Antalya, Turkey


Objective(s): We thought that astaxanthin (ASX) might be a protective agent in oxidative stress damage that develops against ischemia and reperfusion injury in the rat ovary.
Materials and Methods: The experimental groups consisted of healthy, I (Ischemia), I+ASX50, I+ASX100, I/R (Ischemia/Reperfusion), I/R+ ASX50, and I/R+ ASX100. Vascular clamps were applied to the ovaries for 3 hr to induce ischemia. For the reperfusion groups, the clamps were opened and blood flow was restored to the ovaries for 3 hr. At the end of the experiment, biochemical, histopathological, and immunohistochemical analyses were made from the tissue samples taken.
Results: While MDA levels increased significantly in I and I/R groups, SOD levels decreased. It was found that ASX significantly decreased MDA levels and increased SOD activity in treatment groups depending on the dose. Caspase 3, IL-1 β, and IL-6 expressions were severely increased in ischemia and I/R groups, while the severity of I+ASX50 and I/R+ASX100 immunoreactivity was decreased. While severe hemorrhage areas were observed in I and IR groups, minimal hemorrhage areas were observed in the treatment groups, especially in I/R+ASX100 groups. In addition, inflammatory cells and necrotic cells in the I/R group were not observed in I/R+ASX50 and I/R+ASX100 groups.
Conclusion: As a result, it was determined that ASX has a strong protective role against oxidative damage in the treatment of ovarian ischemia-reperfusion injury.


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