Effect of whole body vibration therapy on circulating serotonin levels in an ovariectomized rat model of osteoporosis

Document Type : Original Article


1 1Department of Rehabilitation, General Hospital of Guangzhou Military Command of PLA, Guangzhou, China

2 2Key Laboratory of Orthopaedics & Traumatology, Guangzhou University of Traditional Chinese Medicine, Guangzhou, China



Objective(s): Studies have reported that whole body vibration (WBV) played a vital role in bone remodeling. Circulating serotonin is also involved in negative regulating bone mass in rodents and humans. However, both WBV and inhibition of serotonin biosynthesis may suppress receptor activator of nuclear factor-kappaB ligand (RANKL)-induced osteoclastogenesis in vitro
. The purpose of the current study was to investigate the effect of WBV therapy on the levels of serum serotonin in ovariectomized rats.
Materials and Methods:
Thirty-six-month-old female Sprague Dawley rats weighing 276.15±37.75 g were ovariectomized to induce osteoporosis, and another ten rats underwent sham operation to establish sham control (SHAM) group. After 3 months, ovariectomized rats were divided into three subgroups and then separately treated with WBV, Alendronate (ALN) and normal saline (OVX), SHAM group was given normal saline. After 6 weeks of treatment, rats were sacrificed. Serum serotonin, RANKL, bone turnover markers, and bone mineral density (BMD), bone strength were evaluated.
The serum serotonin level was significantly lower in WBV group than OVX and ALN groups (PP<0.001). RANKL levels significantly decreased in WBV and ALN groups compared to OVX group (PPP

These data indicated that WBV enhanced the bone strength and BMD in ovariectomized rats most likely by reducing the levels of circulating serotonin


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