Silicon nanoparticles: A promising approach for control of Pseudomonas aeruginosa biofilms

Document Type : Original Article

Authors

1 Aquatic Diagnostic and Research Center Bahria University Karachi 75260 Pakistan

2 Department of Microbiology, University of Karachi, Pakistan

3 Microbiology Section, PCSIR Laboratories Complex Karachi-75280 Pakistan

4 College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China

5 Centre for Development of Laboratory Equipment, PCSIR Laboratories Complex Karachi-75280 Pakistan

6 Institute of Microbiology and Molecular Biology, Bahauddin Zakariya University, Multan, Pakistan

7 Department of Prosthodontics, Dow International Dental Collage (DUHS) Karachi, Sindh-Pakistan

8 Department of Biological Sciences, Government Degree Collage Larkana, Sindh-Pakistan

9 Department of Physics and Oxide Research Center, Hankuk University of Foreign Studies, Yongin, 17035, Republic of Korea

Abstract

Objective(s): The current study aimed to investigate the control and treatment of biofilm-producing isolates of Pseudomonas aeruginosa using silicon nanoparticles (SiNPs). 
Materials and Methods: Biofilm-producing isolates of P. aeruginosa were recovered from various food samples and identified through fluorescent green colony formation on selective and differential media, as well as the amplification of oprI and oprL genes. Tube methods, Congo-red agar method, and scanning electron microscopy (SEM) were used to study biofilm phenotypes. The effect of SiNPs was evaluated by broth dilution assay.
Results: The biofilm assay revealed that these isolates formed biofilms on glass surfaces within 72 hr of incubation. Scanning electron micrographs showed that the biofilm communities were composed of multicellular clusters of P. aeruginosa encased in matrix material. However, these isolates were unable to form biofilms on SiNPs-coated surfaces. The results showed that the planktonic isolates of P. aeruginosa were comparatively sensitive to the antibacterial properties of SiNPs, with minimum inhibitory concentration (MIC) ranging from 100 to 200 µg/ml. Contrarily, the biofilms were found to be 500 times more tolerant to the highest concentration of SiNPs (MIC of 500 µg/ml) and were more resistant. Under static conditions, the sedimentation of SiNPs resulted in their ineffectiveness. However, under shaking conditions, the biofilms were effectively dispersed and the cells were lysed. The results showed that SiNPs were effective against both the planktonic and the metabolically inactive forms of P. aeruginosa.
Conclusion: This study suggests that SiNPs could be a useful tool for preventing the formation of biofilms and removing pre-existing biofilms.

Keywords

Main Subjects

References

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Iranian Journal of Basic Medical Sciences
Volume 26, Issue 12
December 2023
Pages 1475-1483

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