Effect of electroacupuncture combined with sulforaphane in the treatment of sarcopenia in SAMP8 mice

Document Type : Original Article

Authors

1 Department of TCM Acupuncture, Huzhou Central Hospital & Affiliated Central Hospital Huzhou University, 313000, Huzhou, China

2 Department of Pathology, Huzhou Central Hospital & Affiliated Central Hospital Huzhou University, 313000, Huzhou, China

3 Department of Neurology, Huzhou Central Hospital & Affiliated Central Hospital Huzhou University, 313000, Huzhou, China

Abstract

Objective(s): Skeletal muscles mitochondrial dysfunction is the main cause of sarcopenia. Both electroacupuncture (EA) and sulforaphane (SFN) have been shown to improve oxidative stress and inflammation levels to maintain mitochondrial function, but the effects and mechanisms of their combination on sarcopenia are unclear. This study aimed to investigate the regulatory effects of EA combined with SFN on sarcopenia.
Materials and Methods: SAMP8 mice were used and intervened with EA or SFN, respectively, and Masson and HE staining were used to observe pathological changes in skeletal muscle tissue. Transmission electron microscopy was used to detect tissue mitochondrial changes. TUNEL staining was used to assess apoptosis. The biochemical and molecular content was tested by ELISA, western blot, and qRT-PCR.
Results: The results showed that oxidative stress, apoptosis, and IL-6, TNF-α, Atrogin-1, and MuRF1 levels in skeletal muscles cells were suppressed and mitochondrial damage was repaired after EA or SFN intervention. In addition, we found that the above changes were associated with the activation of the AMPK/Sirt1/PGC-1α pathway in skeletal muscle tissues, and the promotion effect of combined EA and SFN intervention was more significant.
Conclusion: In conclusion, this study found that EA combined with SFN mediated the repair of mitochondrial damage through activation of the AMPK/Sirt1/PGC-1α pathway, thereby alleviating skeletal muscles morphology and function in sarcopenia. This study combines EA with SFN, which not only broadens the use of electroacupuncture and SFN but also provides a scientific experimental basis for the treatment of sarcopenia.v

Keywords

Main Subjects

References

1. Santo RCDE, Baker JF, Santos LPD, Silva MMM, Xavier RM. Sarcopenia in immune-mediated rheumatic diseases-review. J Clin Rheumatol 2022; 29:354-362.
2. O’Donovan G, Sarmiento OL, Hessel P, Muniz-Terrera G, Duran-Aniotz C, Ibáñezet A. Associations of body mass index and sarcopenia with screen-detected mild cognitive impairment in older adults in Colombia. Front Nutr 2022; 9:1-10.
3. Melouane A, Yoshioka M, St-Amand J. Extracellular matrix/mitochondria pathway: A novel potential target for sarcopenia. Mitochondrion 2020; 50:63-70.
4. Picca A, Calvani R, Bossola M, Allocca E, Menghi A, Pesce V, et al. Update on mitochondria and muscle aging: All wrong roads lead to sarcopenia. Biol Chem 2018; 399:421-436.
5.    Ferri E, Marzetti E, Calvani R, Picca A, Cesari M, Arosio B. Role of age-related mitochondrial dysfunction in sarcopenia. Int J Mol Sci 2020; 21:5236-5248.
6.    Ahn B, Ranjit R, Kneis P, Xu H, Piekarz KM, Freeman WM, et al. Scavenging mitochondrial hydrogen peroxide by peroxiredoxin 3 overexpression attenuates contractile dysfunction and muscle atrophy in a murine model of accelerated sarcopenia. Aging Cell 2022; 21:1-13.
7.    Mahmoud Moustafa E, Rashed ER, Rashed RR, Omar NN. Piceatannol promotes hepatic and renal AMPK/SIRT1/PGC-1α mitochondrial pathway in rats exposed to reserpine or gamma-radiation. Int J Immunopathol Pharmacol 2021; 35:1-15.
8.    Pang Y, Zhang L, Liu Q, Peng H, He J, Jin H, et al. NRF2/PGC-1α-mediated mitochondrial biogenesis contributes to T-2 toxin-induced toxicity in human neuroblastoma SH-SY5Y cells. Toxicol Appl Pharmacol 2022; 451:116167.
9.    Liu H, Zhu S, Han W, Cai Y, Liu C. DMEP induces mitochondrial damage regulated by inhibiting Nrf2 and SIRT1/PGC-1 α signaling pathways in HepG2 cells. Ecotoxicol Environ Saf 2021; 221:1-9.
10.    Huang DD, Yan XL, Fan SD, Chen XY, Yan JY, Dong QT, et al. Nrf2 deficiency promotes the increasing trend of autophagy during aging in skeletal muscle: A potential mechanism for the development of sarcopenia. Aging (Albany NY) 2020; 12:5977-5991.
11.    Liu Y, Zhang D, Li X, Xiao J, Gao L. Enhancement of ultrasound-assisted extraction of sulforaphane from broccoli seeds via the application of microwave pretreatment. Ultrason Sonochem 2022; 87:1-7.
12.    Yang PM, Cheng KC, Huang JY, Wang SY, Lin YN, Tseng YT, et al. Sulforaphane inhibits blue light-induced inflammation and apoptosis by upregulating the SIRT1/PGC-1α/Nrf2 pathway and autophagy in retinal pigment epithelial cells. Toxicol Appl Pharmacol 2021; 421:1-11.
13.    Taheri M, Roudbari NH, Amidi F, Parivar K. Investigating the effect of sulforaphane on AMPK/AKT/NRF2 pathway in human granulosa-lutein cells under H(2)O(2)-induced oxidative stress. Eur J Obstet Gynecol Reprod Biol 2022; 276:125-133.
14.    Taheri M, Hayati Roudbari N, Amidi F, Parivar K. The protective effect of sulforaphane against oxidative stress in granulosa cells of patients with polycystic ovary syndrome (PCOS) through activation of AMPK/AKT/NRF2 signaling pathway. Reprod Biol 2021; 21: 100563.
15.    Bose C, Alves I, Singh P, Palade PT, Carvalho E, Børsheim E, et al. Sulforaphane prevents age-associated cardiac and muscular dysfunction through Nrf2 signaling. Aging Cell 2020; 19:1-14.
16.    Duan HR, Li R, Song SS, Hu SQ, Zhuang ST, Li QY. [Electroacupuncture improves glucose and lipid metabolism by regulating APN/AMPK/PPARα signaling of skeletal muscle in Zucker diabetic obese rats). Zhen Ci Yan Jiu 2021; 46:907-913.
17.    Zhu ZW, Tang CL, Li XH, Yang ZX, Yang YH, Pang P, et al. [Effects of electroacupuncture on proangiogenesis process and protein turnover in a mouse model of sarcopenia). Zhen Ci Yan Jiu 2020; 45:973-979.
18.    Zhang YJ, Li J, Huang W, Mo GY, Wang LH, Zhuo Y, et al. [Effect of electroacupuncture combined with treadmill exercise on body weight and expression of PGC-1α irisin and AMPK in skeletal muscle of diet-induced obesity rats). Zhen Ci Yan Jiu 2019; 44:476-80.
19.    Liu J, Liu J, Wang G, Liu G, Zhou H, Fan Y, et al. Electroacupuncture at guanyuan (CV 4), zusanli (ST 36) and baihui (DU 20) regulate the aging-related changes in gene expression profile of the hippocampus in sub-acutely aging rats. PLoS One 2018; 13:1-18.
20.    Huang Y, Wu B, Shen D, Chen J, Yu Z, Chen C. Ferroptosis in a sarcopenia model of senescence accelerated mouse prone 8 (SAMP8). Int J Biol Sci 2021; 17:151-162.
21.    Morita Y, Ishida T, Morisawa S, Jobu K, Ou Y, Fujita H, et al. Juzentaihoto suppresses muscle atrophy and secreased motor function in SAMP8 mice. Biol Pharm Bull 2021; 44:32-38.
22.    Chen LH, Chang SS, Chang HY, Wu CH, Pan CH, Chang CC, et al. Probiotic supplementation attenuates age-related sarcopenia via the gut-muscle axis in SAMP8 mice. J Cachexia Sarcopenia Muscle 2022; 13:515-531.
23.    Caballero-García A, Pascual-Fernández J, Noriega-González DC, Bello HJ, Pons-Biescas A, Roche E, et al. L-citrulline supplementation and exercise in the management of sarcopenia. Nutrients 2021; 13:3133-3145.
24.    Munguia L, Rubio-Gayosso I, Ramirez-Sanchez I, Ortiz A, Hidalgo I, Gonzalez C, et al. High flavonoid cocoa supplement ameliorates plasma oxidative stress and inflammation levels while improving  mobility and quality of life in older subjects: A double-blind randomized clinical trial. J Gerontol A Biol Sci Med Sci 2019; 74:1620-1627.
25.    Han YG, Qin X, Zhang T, Lei M, Sun FY, Sun JJ, et al. Electroacupuncture prevents cognitive impairment induced by lipopolysaccharide via inhibition of oxidative stress and neuroinflammation. Neurosci Lett 2018; 683:190-195.
26.    Ynag JY, Jiang J, Tian H, Wang ZD, Ren JY, Liu H, et al. [Effect of electroacupuncture on learning-memory ability and expression of IL-1β, IL-6 and TNF-α in hippocampus and spleen in mice with alzheimer’s disease). Zhen Ci Yan Jiu 2021; 46:353-361.
27.    Wang K, Wu SB, Cui S, Xiang SY, Wu X, Zhou MQ. [Effect of electroacupuncture on hippocampal IL-6, IL-1β, TNF-α and norepinephrine levels in acute myocardial ischemia rats). Zhen Ci Yan Jiu 2018; 43:365-369.
28.    Subedi L, Lee JH, Yumnam S, Ji E, Kim SY. Anti-Inflammatory effect of sulforaphane on LPS-activated microglia potentially through JNK/AP-1/NF-kB inhibition and Nrf2/HO-1 activation. Cells 2019; 8:194-211.
29.    Jiang B, Yang YJ, Dang WZ, Li H, Feng GZ, Yu XC, et al. Astragaloside IV reverses simvastatin-induced skeletal muscle injury by activating the AMPK-PGC-1α signalling pathway. Phytother Res 2020; 34:1175-1184.
30.    Zhang, YJ, Wu Q. Sulforaphane protects intestinal epithelial cells against lipopolysaccharide-induced injury by activating the AMPK/SIRT1/PGC-1α pathway. Bioengineered 2021; 12:4349-4360.
31.    Dong JZ, Wei YT, Xu HY, Zhang Y, Yong RL, Xue YN, et al. [Electroacupuncture of “zusanli” (ST 36) raises muscular force by adjusting AMPK/PGC-1α signaling in rats with chronic fatigue syndrome). Zhen Ci Yan Jiu 2018; 43:335-340.
32.    Wang M, Jiang R, Liu J, Xu X, Sun G, Zhao D, et al. 20(s) │ginseonside│Rg3 modulation of AMPK/FoxO3 signaling to attenuate mitochondrial dysfunction in a dexamethasone│injured C2C12 myotube│based model of skeletal atrophy in vitro. Mol Med Rep 2021; 23:306-316.
33.    Zhang SF, Zhang Y, Li B, Chen N. Physical inactivity induces the atrophy of skeletal muscle of rats through activating AMPK/FoxO3 signal pathway. Eur Rev Med Pharmacol Sci 2018; 22:199-209.
Iranian Journal of Basic Medical Sciences
Volume 27, Issue 5
May 2024
Pages 560-566

Files

Share

How to cite

Statistics