Potential antiaging activity of secretome gel of human Wharton’s jelly mesenchymal stem cells (hWJ-MSCs) in UV-induced mice models

Document Type : Original Article


1 Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia

2 Department of Neurosurgery, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia

3 Oncology and Stem Cell Working Group, Faculty of Medicine, Universitas Padjadjaran-Dr. Hasan Sadikin Hospital, Bandung, West Java, Indonesia

4 Faculty of Pharmacy, Pancasila University, South Jakarta, Indonesia

5 Biomolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia

6 Faculty of Pharmacy, University of Surabaya, Universitas Surabaya, Surabaya, East Java, Indonesia

7 Biomedical Engineering Department of Electrical Engineering, Faculty of Engineering University of Indonesia, Jakarta, Indonesia

8 Clinical Pathology Department, School of Medicine and Health Sciences, Atma Jaya Catholic University, Jakarta, Indonesia

9 Eijkman Research Center for Molecular Biology, National Research and Innovation Agency, Bogor, West Java, Indonesia

10 Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran

11 Experimental Cancer Medicine, Institution for Laboratory Medicine, Karolinska, Institute, Stockholm, Sweden


Objective(s): Skin aging is a degenerative process that can be induced by UV irradiation. UV radiation can produce reactive oxidate stress which causes premature aging. This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJ-MSCs) in a UVB-induced mice model. 
Materials and Methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min twice daily for 14 days. The gel was topically applied to the mice’s dorsal skin. Two treatments of secretome gel: secretome 1 is applied once and secretome 2 is applied twice daily after UVB radiation. TGF-β1, IL-10, and IL-18 gene expression was determined using RT-PCR. Hematoxylin Eosin staining was used to observe the inflammation and collagen density of skin tissue. An immunohistochemistry assay was used to analyze the protein expression of P53, COL4A1, MMP-2, and MMP-13. The data were statistically analyzed using the ANOVA test followed by the Tukey post hoc test (P<0.05). 
Results: UVB induction caused loss of collagen, increasing inflammation and high expression of aging mediators. SC increased the gene expression of TGF-β1 and IL-10 and decreased IL-18 gene expression. Histopathological tests showed that SG increased collagen density, lowered inflammation, and repaired cell damage in skin tissue. Immunohistochemistry test showed that SC decreased MMP-2, MMP-13, and P53 expression, in contrast, increased COL4A1. 
Conclusion: The secretome gel of hWJ-MSCs showed antiaging activities with potential for preventing and curing skin aging.


Main Subjects

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