Spectrofluorimetric determination of cefixime using terbium-danofloxacin probe

Document Type : Original Article

Authors

1 Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran

2 Tuberculosis and Lung Disease Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

3 Drug Applied Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

Objective(s):Cefixime (Cfx), is a semi-synthetic third-generation oral cephalosporin antibiotic that is prescribed for the treatment of susceptible infections. There are some procedures for the determination of Cfx in pharmaceutical formulations and biological samples. Herein a spectrofluorimetric method was proposed for Cfx determination based on the fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx.
Materials and Methods: Cfx was detected based on fluorescence quenching of terbium-danofloxacin (Tb3+-Dano) in the presence of Cfx with maximum excitation and emission wavelengths at 347 nm and 545 nm, respectively. The quenched fluorescence intensity of Tb3+- Dano system is proportional to the concentration of Cfx. The optimum conditions for the determination of Cfx were studied.
Results: The maximum response was achieved under optimum conditions of [Tris buffer]= 0.008 mol/l (pH 6.5), [Tb3+]=1×10-4 mol/l  and [Dano]=1×10-4 mol/l. The developed method was evaluated in terms of accuracy, precision and limit of detection. The linear concentration ranges for quantification of Cfx were 8.8×10-8-8.8×10-7 mol/l and 1.1×10-7-8.8×10-7 mol/l in standard and human serum samples with the detection limits (S/N=3) of 2.8×10-8 mol/l and 3.9×10-8 mol/l, respectively. The Cfx was determined in pharmaceutical tablets and spiked serum samples and the results were satisfactory.  
Conclusion: This method is simple, practical and relatively interference-free for determination of Cfx in pharmaceutical tablets and serum samples.

Keywords

References

1. Knapp CC, Sierra-Madero J, Washington JA. Antibacterial activities of cefpodoxime, cefixime, and ceftriaxone. Antimicrob Agents Chemother 1988; 32:1896-1898.
2. Sanders CC. β-Lactamase stability and in vitro activity of oral cephalosporins against strains possessing well-characterized mechanisms of resistance. Antimicrob Agents Chemother 1989; 33:1313-1317.
3. Brogden RN, Campoli-Richards DM. Cefixime. A review of its antibacterial activity, pharmacokinetic properties and therapeutic potential. Drugs 1989; 38:524-550.
4. Al-Momani IF. Spectrophotometric determination of selected cephalosporins in drug formulations using flow injection analysis. J Pharm Biomed Anal 2001; 25:751-757.
5. Shankar DG, Sushma K, Lakshmi RV, Srinivasa Rao Y, Reddy MN, Murthy TK. Spectrophotometric determination of cefixime trihydrate. Asian J Chem 2001; 13:1649-1651.
6. Shah PB, Pundarikakshudu K. Spectrophotometric, difference spectroscopic, and high-performance liquid chromatographic methods for the determination of cefixime in pharmaceutical formulations. J AOAC Int 2006; 89:987-994.
7. Khan A, Iqbal Z, Khan MI, Javed K, Ahmad L, Shah Y, et al. Simultaneous determination of cefdinir and cefixime in human plasma by RP-HPLC/UV detection method: Method development, optimization, validation, and its application to a pharmacokinetic study. J Chromatogr B Anal Technol Biomed Life Sci 2011; 879:2423-2429.
8. Eric-Jovanovic S, Agbaba D, Zivanov-Stakic D , Vladimirov S. HPTLC determination of ceftriaxone, cefixime and cefotaxime in dosage forms. J Pharm Biomed Anal 1998; 18:893-898.
9. Jain R, Gupta VK, Jadon N, Radhapyari K. Voltammetric determination of cefixime in pharmaceuticals and biological fluids. Anal Biochem 2010; 407:79-88.
10. Golcu A, Dogan B, Ozkan SA. Anodic voltammetric behavior and determination of cefixime in pharmaceutical dosage forms and biological fluids. Talanta 2005; 67:703-712.
11. Madhusudana Reddy T, Sreedhar M, Jayarama Reddy S. Voltammetric behavior of Cefixime and Cefpodoxime Proxetil and determination in pharmaceutical formulations and urine. J Pharm Biomed Anal 2003; 31:811-818.
12. El Walily AFM, Gazy AAK, Belal SF, Khamis EF. Use of cerium (IV) in the spectrophotometric and spectrofluorimetric determinations of penicillins and cephalosporins in their pharmaceutical preparations. Spectrosc Lett 2000; 33:931-948.
13. Bebawy LI, El Kelani K, Fattah LA. Fluorimetric determination of some antibiotics in raw material and dosage forms through ternary complex formation with terbium (Tb3+). J Pharm Biomed Anal 2003; 32:1219-1225.
14. Bukhari N, Al-Warthan AA, Wabaidur SM, Othman ZA, Javid M, Haider S. Spectrofluorimetric determination of cefixime in pharmaceutical preparation and biological fluids using calcein as a fluorescence probe. Sens Lett 2010; 8:280-284.
15. Elbashir AA, Ahmed SMA, Aboul-Enein HY. New spectrofluorimetric method for determination of cephalosporins in pharmaceutical formulations. J Fluoresc 2012; 22:857-864.
16. Shah J, Jan MR, Shah S, Inayatullah. Spectrofluorimetric method for determination and validation of cefixime in pharmaceutical preparations through derivatization with 2-cyanoacetamide. J Fluoresc 2011; 21:579-585.
17. Ruiz-Medina A, Llorent-Martinez EJ, Ortega-Barrales P, Cordova MLFD. Lanthanide-sensitized luminescence as a promising tool in clinical analysis. Appl Spectrosc Rev 2011; 46:561-580.
18. Rodríguez-Díaz RC, Fernández-Romero JM, Aguilar-Caballos MP, Gómez-Hens A. Determination of fluoroquinolones in milk samples by postcolumn derivatization liquid chromatography with luminescence detection. J Agric Food Chem 2006; 54:9670-9676.
19. Mohammadpour AH, Ramezani M, Tavakoli NA, Malaekeh BN, Amel AF, Hosseinzadeh H. Development and validation HPLC method for determination of protein, a constituent of saffron in human serum samples. Iran J Basic Med Sci 2013; 16:47-55.
20. Manzoori JL, Jouyban A, Amjadi M, Panahi-Azar V, Karami-Bonari AR, Tamizi E. Spectrofluorimetric determination of buparvaquone in biological fluids, food samples and a pharmaceutical formulation by using terbium–deferasirox probe. Food Chem 2011; 126:1845-1849.
21. Manzoori JL, Jouyban A, Amjadi M, Panahi-Azar V, Tamizi E, Vaez-Gharamaleki J. Terbium-sensitized fluorescence method for the determination of deferasirox in biological fluids and tablet formulation. Luminescence 2011; 26:244-250.
22. Bansal S, DeStefano A. Key elements of bioanalytical method validation for small molecules. AAP S J 2007; 9:E109-E114.
23. Ingle JD, Crouch SR. Spectrochemical Analysis. 3th ed. Prentice Hall. Englewood Cliffs: New Jersey: 1988.

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