The Relaxant Effects of Different Methanolic Fractions of Nigella sativa on Guinea Pig Tracheal Chains

Document Type : Original Article

Authors

1 Tuberculosis and Lung Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

2 Department of Physiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

3 Research Centre for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

Abstract

 
Objective(s): In regard to the high incidence of asthma and the side-effects of the drugs used, finding novel treatments for this disease is necessary. Our previous studies demonstrated the preventive effect of Nigella sativa extract on ovalbumin-induced asthma. In addition, water-soluble substances of N. sativa extract and methanol fraction of this plant were responsible for the relaxant effect of this plant on tracheal chains of guinea pigs. Therefore, for the first time, in the present study, in order to identify main constituents of the methanolic extract, the relaxant effects of five different methanolic fractions (20%, 40%, 60%, 80%, and 100%) of N. sativa
 
on tracheal chains of guinea pigs were examined.
 
 
Materials and Methods:
 
 
The relaxant effects of four cumulative concentrations of each fraction (0.8, 1.2, 1.6, and 2.0 g%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.2, 0.4, 0.6, and 0.8 mM) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1) and 10 μM methacholine (group 2).
 
 
Results:
In group 1, all concentrations of only theophylline showed significant relaxant effects but all concentrations of these methanolic fractions showed significant contractile effects compared with that of saline (P<0.001 to P<0.05). However, in group 2, all concentrations of theophylline and these methanolic fractions showed significant relaxant effects compared with that of saline (P<0.001 to P<
 
0.05).
 
 
Conclusion:
These results showed a potent relaxant effect of 20% methanolic fractions from N. sativa
 
on tracheal chains of guinea pigs that were higher than that of theophylline at the used concentrations
 
 

Keywords

References

Bousquet J, Mantzouranis E, Alvaro A. Cruz AA, Aït-Khaled N, Baena-Cagnani CE, Bleecker ER, et al.
Uniform definition of asthma severity, control, and exacerbations: Document presented for the World Health Organization Consultation on severe asthma. J Allergy Clin Immunol 2010; 126:926-938.
2. Goreja WG. Black seed: Nature’s miracle remedy. New York, NY: Amazing herbs press; 2003.
 
3. El-Dakhakhny M. Studies on chemical constitution of Egyptian
 
 
Nigella sativa
L. seeds. II. The essential oil. Planta Med 1963; 11:465- 470.
4. Atta UR, Malik SO. Nigellidine, a new indazol alkaloid from seeds of
 
 
Nigella sativa
. J Res Inst 1995; 36:1993-1996.
5. Atta UR, Malik SO. Nigellimine N-oxide, a new isoquinoline alkaloid from the seeds of
 
 
Nigella sativa
. Hetrocycles 1985; 23:953-955.
6. Ansari AK, Sadiy HAS. Structural studies on a saponin isolated from the seeds of
 
 
Nigella sativa
. Phyto Chem 1989; 7:377-379.
7. Ave-Sina. Law in Medicine, Interpreter; Sharafkhandy A, Teheran: Ministry of Guidance publication; 1990. p.314.
 
8. Ali BH, Blunden G. Pharmacological and toxicological properties of
 
 
Nigella sativa
. Phytother Res 2003; 17:299-305.
9. Labib Salem M. Immunomodulatory and therapeutic properties of the
 
 
Nigella sativa
L. seed. Int Immunopharmacol 2005; 5:1749- 1770.
10. Aqel MB. The relaxing effect of volatile oil of
 
 
Nigella sativa
seed on vascular smooth muscle. Jordan Ser B 1992; 1: 91-100.
11. Aqel MB. Effects of
 
 
Nigella sativa
seeds on intestinal smooth muscle. Inter J Pharmacogn 1993; 31: 55-60.
12. Reiter M, Brandt W. Relaxant effects on tracheal and ileal smooth muscles of the guinea- pig. Arzneimittelforschung 1985; 35:408- 414.
 
13. Boskabady MH, Shahabi M. Bronchodilatory and anticholinergic effects of
 
 
Nigella sativa
on isolated guinea-pig tracheal chains. Iran J Med Sci 1997; 22:127-133.
14. Boskabady MH, Shiravi, N. Inhibitory effect of
 
 
Nigella sativa on histamine (H1
) receptors of isolated guinea pig tracheal chains. Eur Respir J 2000; 16:461s.
15. Boskabady MH, Shirmohammadi B. Effect of
 
 
Nigella sativa
on isolated guinea pig tracheal chains. Arch Iran Med 2002; 5:103-107.
16. Boskabady MH, Shirmohammadi B, Jandaghi P, Kiani S. Possible mechanisms for relaxant effect of aqueous and macerate extracts from
 
 
Nigella sativa
on tracheal chains of guinea pig. BMC Pharmacol 2004; 4:3.
17. Boskabady MH, Kiani S, Jandaghi P. Stimulatory effect of
 
 
Nigella sativa on ß2
-adronceptors of guinea pig tracheal chains. Med J I R Iran 2004; 18:153-158.
18. Boskabady MH, Kiani S, Jandaghi P, Ziaei T, Zarei A. Antitussive of
 
 
Nigella sativa
. Pak J Med Sci 2004; 20:224-228.
19. Boskabady MH, Keyhanmanesh R, Khamneh S, Doostar Y, Khakzad MR. Potential immunomodulation effect of the extract of
 
 
Nigella sativa
on ovalbumin sensitized guinea pigs. J Zhejiang Univ Sci 2011; 12:201-209.
20. Boskabady MH, Keyhanmanesh R, Khamneh S, Ebrahimi MA. The effect of
 
 
Nigella sativa
extract on tracheal responsiveness and lung inflammation in ovalbumin sensitized guinea pigs. Clinics (Sao Paulo) 2011; 66:879-887.
21. Keyhanmanesh R, Boskabady MH, Ebrahimi Saadatloo MA. The contribution of water and lipid soluble substances in the relaxant effects of
 
 
Nigella sativa extract on guinea pig tracheal smooth muscle (in vitro
). Iran J Basic Med Sci 2007; 10:154–161.
22. Boskabady MH, Keyhanmanesh R, Ebrahimi Saadatloo MA. Relaxant effects of different fractions from
 
 
Nigella sativa
L. on guinea pig tracheal chains and its possible mechanism(s). Indian J Exp Biol 2008; 46:805-810.
23. Miyahara Y, Kizawa Y, Sano M, Murakami H. Effect of organic and inorganic ca
 
 
+2
antagonist on acetylcholine induced contraction in malluscan (myfile edulis) smooth muscle. G Pharmacol 1993; 24:1419-1423.
24. Burits M, Bucar F. Antioxidant activity of
 
 
Nigella sativa
essential oil. Phytother Res 2000; 14: 323-328.
25. Thippeswamy NB, Naidu KA. Antioxidant potency of cumin varieties-cumin, black cumin and bitter cumin- on antioxidant systems. Eur Food Res Technol 2005; 220:472-476.
 
26. Yoruk O, Ozabacigil F, Uyanik H, Yasar M, Mutlu V, Altas E,
 
 
et al. Antioxidant Effects of Nigella sativa
in the Treatment of Experimentally Induced Rhinosinusitis. Macedonian J Med Sci 2010; 3:132-137.
 
Iranian Journal of Basic Medical Sciences
Volume 16, Issue 2 - Serial Number 2
February 2013
Pages 123-128

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