Genetic analysis of two STR loci (VWA and TPOX) in the Iranian province of Khuzestan

Document Type : Original Article

Authors

Department of Genetics, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran

Abstract

Objective(s): Short tandem repeat (STR) loci are the most informative DNA genetic markers for attempting to individualize biological material for application in paternity and forensic cases.
Materials and Methods: Blood samples were collected and the total genomic DNA was extracted. The DNA samples were used for genotyping VWA and TPOX STR loci using PCR and polyacrylamide gel electrophoresis.
Results: This report presents allele frequency data and parameters of biological or legal interest, such as heterozygosity value, polymorphic information content (PIC), genetic diversity index (GD) and population parameter (θ) in Arab and non-Arab population of Khuzestan province (Iran) for the loci VWA and TPOX. Blood samples (N= 392 for VWA and N=308 for TPOX) were collected from individuals unrelated throughout Khuzestan province. The loci were genotyped using the polymerase chain reaction (PCR) followed by polyacrylamide gel electrophoresis (PAGE) and silver staining. Chi-square test showed that neither STR loci were in agreement with the Hardy-Weinberg equilibrium.
Conclusion: The examined STR loci in this study have proven a relatively high genetic variation in the Iranian population. The data could be used for construction of a forensic genetic database for the Iranian population.

Keywords

References

1.Beckmann JS, Weber JL. Survey of human and rat microsatellites. Genomics 1992; 12:627-631.
2.Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M. Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods Appl 1993; 3:13-22.
3.Hagelberg E, Gray IC, Jeffreys AJ. Identification of the skeletal remains of a murder victim by DNA analysis. Nature 1991; 352:427-429.
4.Gill P, Kimpton CP, Sullivan KM. A rapid polymerase chain reaction method for identifying fixed specimens. Electrophoresis 1992; 13:173-175.
5.Jeffreys AJ, Allen MJ, Hagelberg E, Sonnberg A. Identification of the skeletal remains of Josef Mengele by DNA analysis. Forensic Sci Int 1992; 56:65-76.
6.Gill P, Sullivan K, Werrett DJ. The analysis of hypervariable DNA profiles: Problems associated with the objective determination of the probability of a match. Hum Genet 1990; 85:75-79.
7.Evett IW, Gill P. A discussion of the robustness of methods for assessing the evidential value of DNA single locus profiles in crime investigations. Electrophoresis 1991;12:226-30.
8.Angers B, Bernatchez L. Combined use of SMM and Non-SMM methods to infer fine structure and evolutionary history of closely related brook charr (salvelinus fontinalis, salmonidae) populations from microsatellites. Mol Biol Evol 1998; 15:143–159.
9.Butler JM. Forensic DNA typing: biology, technology, and genetics of STR markers. 2nd ed. New York: Elsevier; 2005.
10.Gill P. Role of short tandem repeat DNA in forensic casework in the UK-past, present, and future perspectives. BioTechniques 2002; 32:366–372.
11.Jobling MA, Gill P. Encoded evidence: DNA in forensic analysis. Nat Rev Genet 2004; 5:739–751.
12.Butler JM. Genetics and genomics of core short tandem repeat loci used in human identity testing. J Forensic Sci 2006; 51:253-265.
13.Sambrook J, Fritsch E, Maniatis T. Molecular cloning: a laboratory manual. NewYork: Cold Spring Harbor Laboratory Press; 2003.
14.Budowle B, Chakraborty R, Giusti AM, Eisenberg AJ, Allen, RC. Analysis of the VNTR locus D1S80 by the PCR followed by high-resolution PAGE. Am J Hum Genet 1991; 48:137-144.
15.Excoffier L, Laval G, Schneider S. Arlequin, Version 3.1: Software for population genetics data analysis. Genetics and Biometry Laboratory. Department of Anthropology, University of Berne; 2006. Available at: http://cmpg.unibe.ch/software/arlequin3.
16.Liu k, Muse SV. PowerMarker: Integrated analysis environment for genetic marker data. Bioinformatics 2005; 21:2128-2129.
17.Gelehrter TD, Collins FS, Ginsburg D. Principles of Medical Genetics. 2nd ed. Baltimore: Williams & Wilkins; 1998 p. 48-49.
18.Bittles AH. The impact of consanguinity on the Indian population. Indian J Hum Genet 2002; 8:45-51.
19.Vallian S, Moeini H. Genotyping of five polymorphic STR loci in Iranian province of Isfahan. J Sci 2006; 17:113-117.
20.Hedjazia A, Nikbakhta A, Hosseinia M, Hoseinzadeha A, Hosseini SMV. Allele frequencies for 15 autosomal STR loci in Fars province population, southwest of Iran. Leg Med 2013; 15:226–228.
21.Souto L, Gusmao L, Amorim A, Ferreira E, CorteReal F, Vieira DN, et al. POP-Gene TIMOR: first forensic DNA marker study of East-Timor people. Int Congr Ser 2004; 1261:201– 203.
22.Gustavo M, Vazquez E, Schaller C, Quevedo N. Genetic data on 11 STRs (CSF1PO, TPOX, TH01, F13A01, FESFPS, VWA, D16S539, D7S820, D13S317, F13B, LPL) in an Argentine northeast population. Forensic Sci Int 2003; 133:254–255.
23.Silva C, Castro-Oliveira P, Montiel R, Lima M. Population genetics of 5 STR loci (CD4, TPO, VWA, F13A1 and MBPB) in S. Miguel Island (Azores, Portugal). Antropologia Portuguesa 2003; 20/21:307-321.
24.Deng YJ, Yan JW, Yu XG, Li YZ, Mu HF, Huang YQ, et al. Genetic analysis of 15 STR loci in Chinese Han population from West China. Genomics Proteomics Bioinformatics 2007; 5:66-69.
25.Lahiani NM, Rebaı A, Makni H. Data for 10 autosomal STR markers in South Tunisian population. Forensic Sci Int 2006; 164:254-256.
26.Sanou I, Nunez G, Rodrıguez A, Silva S, Garcıa O, Uriarte J, et al. STR data for 11 autosomal STR markers from Costa Rica, Central America. Forensic Sci Int 2005; 153:272–273.
27.El-Alfy SH, Abd El-Hafez AF. Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer. J Genet Eng Biotechnol 2012; 10:101–112.
28.Martınez GG, Schaller LC, Vazquez LE, Bolea M, Martınez-Jarreta B. Reference database of hypervariable STR Loci in the Entre Rı´os Province of Argentina. Int Congr Ser 2006; 1288:355–357.
29.Dong L, Liu XX, Zhang HH, Bo R, Wei G, Zhang LS. Genetic data for 15 STR loci in Uygur ethnic group of northwest China. Rom J Leg Med 2010; 18:59–62.
30.Mancuso DJ, Tuley EA, Westfield LA, Worrall NK, Shelton-Inloes BB, Sorace JM, et al. Structure of the gene for human von Willebrand factor. J Biol Chem 1989; 264:19514-19527.
31.O'Malley KL, Anhalt MJ, Martin BM, Kelsoe JR, Winfield SL, Ginns EI. Solation and characterization of the human tyrosine hydroxylase gene: identification of 5′ alternative splice sites responsible for multiple mRNAs. Biochemistry 1987; 26:6910-6914.
32.Anker R, Steinbrueck T, Donis-Keller H. Tetranucleotide repeat polymorphism at the human thyroid peroxidase (hTPO) locus. Hum Mol Genet 1992; 1:137.
Iranian Journal of Basic Medical Sciences
Volume 17, Issue 8 - Serial Number 8
August 2014
Pages 583-587

Files

Share

How to cite

Statistics