The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells

Authors

1 Department of Biology & Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran

2 Developmental Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

3 Department of Medical Genetics & Medical Genetic Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Biology, Research & Science Tehran Branch, Islamic Azad University, Tehran, Iran

Abstract

Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed   to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage.
Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concentration of extract. After 21 days, Alizarin red staining, alkaline phosphatase assay and RT-PCR were performed. The results were analyzed by ANOVA software and P value Results: Morphological methods revealed that appropriate concentrations of extract increased osteogenic differentiation in a dose-dependent manner. RT-PCR revealed that extract without or with osteogenic medium due to osteopontin expression had a potential role in osteogenesis.
Conclusion: Based on our data it concluded that S. cucumber extract stimulated Bone marrow mesenchymal cells to differentiate into osteogenic lineage without existence of osteogenic medium

Keywords

References

1. Kawaguchi H, Hirachi A, Hasegawa N, Iwata T, Hamaguchi H, Shiba H, et al. Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells. J Periodontal 2004; 75:281-287.
2. Cragg GM, Newman DJ. Natural products : A continuing source of novel drug leads . Biochim Biophys Acta 2013; 830:3670–3695.
3. Venugopal V. Marine products for healthcare: functional and bioactive nutraceutical compounds from the ocean. J Aquat Food Product Technol 2010; 19:48-54.
4. Shaifuzain AR, Amran AS, Muzaffar TMS, Tengku AR, Shaifulizan. Effect of oral sea cucumber (Stichopus sp1) extract on fracture healing. Malays J Med Sci 2007; 14:172.
5. Kariya Y, Mulloy B, Imai K, Tominaga A, Kaneko T, Asari A, Suzuki K, et al. Isolation and partial characterization of fucan sulfates from the body wall of sea cucumber Stichopusjaponicus and their ability to inhibit osteoclastogenesis. Carbohydr Res 2004; 339:1339-1346.
6. Yamada S, Sugahara K. Potential therapeutic application of chondroitin sulfate/dermatan sulfate. Curr Drug Discov Technol 2008; 5:289–301.
7. Chen SG, Li GY, Yin LA, Huang WC, Dong P, Xu J,              et al. Identification of eight species of sea cucumber chondroitin sulfates by high temperature ~1H NMR. J Instrumental Analysis 2010; 29-38.
8. Hu J, Feng K, Liu X, Ma PX. Chondrogenic and osteogenic differentiations of human bone marrow-derived mesenchymal stem cells on a nanofibrous scaffold with designed pore network. Biomaterials 2009; 30:5061-5067.
9. Dragoo JL, Samimi B, Zhu M, Hame SL, Thomas BJ, Lieberman JR, et al. Tissue-engineered cartilage and bone using stem cells from human infrapatellar fat pads. J Bone Joint Surg Br 2003; 85:740-747.
10. Eijken M, Koedam M, van Driel M, Buurman CJ, Pols HA, van Leeuwen JPV.The essential role of glucocorticoids for proper human osteoblast differentiation and matrix mineralization. Mol Cell Endocrinol 2006; 248:87-93.
11. Byuna MR, Kim AR, Hwang JH, Sung MK, Lee YK, Hwang BS, et al. Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation. FEBS Lett 2012; 586:1086–1092.
12. Zhang DW, Cheng Y, Wang  NL, Zhang JC, Yang MS, Yao XS. Effects of total flavonoids and flavonol glycosides from Epimedium koreanum Nakai on the proliferation and differentiation of primary osteoblasts. Phytomedicine 2008; 15:55–61.
13.  Ryu B, Qian ZJ, Kim SK. Purification of a peptide from seahorse , that inhibits TPA-induced MMP , iNOS and COX-2 expression through MAPK and NF-  B activation, and induces human osteoblastic and chondrocytic differentiation. Chem Biol Interact 2010; 184:413- 422.
14. Park SJ, Lee KW, Lim DS, Lee S. The sulfated polysaccharide fucoidan stimulates osteogenic differentiation of human adipose-derived stem cells. Stem Cells Dev 2011; 21:2204-2211.
15. Shahrulazua A, Samsudin AR, Iskandar MA, Amran AS. The in vitro effects of sea cucumber (Stichopus sp1) extract on human osteoblast cell line. J Malay Ortho  2013; 7:41-48.
Iranian Journal of Basic Medical Sciences
Volume 17, Issue 8 - Serial Number 8
August 2014
Pages 626-631

Files

Share

How to cite

Statistics