Association of ADAM33 gene polymorphisms with allergic asthma

Document Type : Original Article

Authors

1 Allergy Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Division of Human Genetics, Immunology Research Center, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

3 Bushehr University of Medical Science, School of Medicine. Allergy and clinical Immunology. Department, Bushehr, Iran

4 Department of Pathology, Omid Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s): Asthma results from the interaction between genetic and environmental factors. ADAM33 gene on chromosome 20p13 is associated with asthma and airway hyperresponsiveness.
Materials and Methods: This is a case-control study, where four SNPs S1 (rs3918396), T1 (rs2280091), T2 (rs2280090), V4 (rs2787094) of ADAM33 gene have been assessed in patients with allergic asthma and normal controls (95 patients and 86 normal). Blood samples of these participants have been genotyped by PCR and the RFLP method.
Results: There was no association between asthmatic patients and polymorphisms of alleles, genotypes and haplotypes of the ADAM33 gene. When categorizing the asthmatic patients in severe, moderate and mild groups, associations in the subcategories of asthmatic patients were found. There were associations between polymorphisms of C allele of T1 SNP with severe asthmatic patients and G allele of V4 SNP with moderate asthmatics respectively (P=0.006, P=0.01). There was a significant association between sensitivity to mite and polymorphism of C allele of T1 SNP (P=0.02). Besides, there was a significant association between sensitivity to weeds and genotype GG of V4 SNP (P=0.05).
Conclusion: Polymorphisms of ADAM33 gene might be associated with severe asthma and sensitivity to aeroallergens in northeast of Iran, but further studies are needed to determine the polymorphisms in this area and other regions of our country.

Keywords

References

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Iranian Journal of Basic Medical Sciences
Volume 17, Issue 9 - Serial Number 9
September 2014
Pages 716-721

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