Safranal-loaded solid lipid nanoparticles: evaluation of sunscreen and moisturizing potential for topical applications

Document Type : Original Article


1 Department of Food and Drug Control, Mashhad University of Medical Sciences, Mashhad, Iran

2 School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

3 Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

4 Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran


Objective(s): In the current study, sunscreen and moisturizing properties of solid lipid nanoparticle (SLN)-safranal formulations were evaluated.
Materials and Methods:Series of SLN were prepared using glyceryl monostearate, Tween 80 and different amounts of safranal by high shear homogenization, and ultrasound and high-pressure homogenization (HPH) methods. SLN formulations were characterized for size, zeta potential, morphology, thermal properties, and encapsulation efficacy. The Sun Protection Factor (SPF) of the products was determined in vitro using transpore tape. The moisturizing activity of the products was also evaluated by corneometer.
Results: The SPF of SLN-safranal formulations was increased when the amount of safranal increased. Mean particle size for all formulas was approximately 106 nm by probe sonication and 233 nm using HPH method. The encapsulation efficiency of safranal was around 70% for all SLN-safranal formulations.
Conclusion: The results conclude that SLN-safranal formulations were found to be effective for topical delivery of safranal and succeeded in providing appropriate sunscreen properties.


1. Varvaresou A. Percutaneous absorption of organic sunscreens. J Cosmet Dermatol 2006; 5:53-57.
2. Movileanu L,  Neagoe I, Flonta ML. Interaction of the antioxidant flavonoid quercetin with planar lipid bilayers. Int J Pharm 2000; 205:135-146.
3. Tabrizi H,  Mortazavi SA, Kamalinejad M. An in vitro evaluation of various Rosa damascena flower extracts as a natural antisolar agent. Int J Cosmet Sci 2003; 25:259-265.
4. Katiyar SK, Elmets CA. Green tea polyphenolic antioxidants and skin photoprotection. Int J Oncol 2001; 18:1307-1313.
5. Golmohammadzadeh S,  Imani F,  Hosseinzadeh H, Jaafari MR. Preparation, characterization and evaluation of sun protective and moisturizing effects of nanoliposomes containing safranal. Iran J Basic Med Sci 2011; 14 521-533.
6. Golmohammadzadeh S,  Jaafari MR, Hosseinzadeh H. Does Saffron have antisolar and moisturizing effects? Iran J Pharm Res 2010; 9:133-140.
7. Puglia C,  Blasi P,  Rizza L,  Schoubben A,  Bonina F,  Rossi C, et al. Lipid nanoparticles for prolonged topical delivery: an in vitro and in vivo investigation. Int J Pharm 2008; 357:295-304.
8. Gokce EH,  Korkmaz E,  Tuncay-Tanriverdi S,  Dellera E, Sandri G, Bonferoni MC, et al. A comparative evaluation of coenzyme Q10-loaded liposomes and solid lipid nanoparticles as dermal antioxidant carriers. Int J Nanomedicine 2012; 7:5109-5117.
9. Jenning V, Schäfer-Korting M, Gohla S. Vitamin A-loaded solid lipid nanoparticles for topical use: Drug release properties. J Control Release 2000; 66:115-126.
10. Mosallaei N, Jaafari MR, Hanafi-Bojd MY,  Golmohammadzadeh S, Malaekeh-Nikouei B. Docetaxel-loaded solid lipid nanoparticles: preparation, characterization, in vitro, and in vivo evaluations. J Pharm Sci 2013; 102:1994-2004.
11. Aggarwal N, Goindi S. Preparation and in vivo evaluation of solid lipid nanoparticles of griseofulvin for dermal use. J Biomed Nanotechnol 2013; 9:564-576.
12. Wissing SA, Muller RH. A novel sunscreen system based on tocopherol acetate incorporated into solid lipid nanoparticles. Int J Cosmet Sci 2001; 23:233-243.
13. Smith NB. Perspectives on transdermal ultrasound mediated drug delivery. Int J Nanomed 2007; 2:585-594.
14. Prow TW,  Grice JE,  Lin LL,  Faye R,  Butler M,  Becker W, et al. Nanoparticles and microparticles for skin drug delivery. Adv Drug Deliv Rev 2011; 63:470-491.
15. Muller RHm,  Radtke M, Wissing SA. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. AdvDrug Deliv Rev 2002; 54:131-155.
16. Layegh P, Mosallaei N, Bagheri D, Jaafari MR, Golmohammadzadeh S. The efficacy of Isotretinoin-loaded solid lipid nanoparticles in comparison to Isotrex® on acne treatment. Nanomed J 2013;1:38-47.
17. Zougagh M,  Rios A, Valcarcel M. Determination of total safranal by in situ acid hydrolysis in supercritical fluid media: Application to the quality control of commercial saffron. Anal Chim Acta 2006; 578:117-121.
18. Diffey B, Robson J. A new substrate to measure sunscreen protection factors throughout the ultraviolet spectrum. J Soc Cosmet Chem 1989; 40:127-133.
19. Pardeike J,  Hommoss A, Muller RH. Lipid nanoparticles (SLN, NLC) in cosmetic and pharmaceutical dermal products. Int J Pharm 2009; 366:170-184.
20. Souto EB, Muller RH. Cosmetic features and applications of lipid nanoparticles (SLN, NLC). Int J Cosmet Sci 2008; 30:157-165.
21. Xia Q,  Saupe A,  Müller R, Souto E. Nanostructured lipid carriers as novel carrier for sunscreen formulations. Int J Cosmet Sci 2007; 29:473-482.
22. Bach A,  Zach-Maor A, Semiat R. Characterization of iron oxide nanocatalyst in mineralization processes. Desalination 2010; 262:15-20.
23. Golmohammadzadeh S,  Mokhtari M, Jaafari MR. Preparation, characterization and evaluation of moisturizing and UV protecting effects of topical solid lipid nanoparticles. Brazilian J Pharm Sci 2012; 48: 683-690.
24. Marković IS,  Đarmati ZA, Abramović BF. Chemical composition of leaf extracts of Stevia rebaudiana Bertoni grown experimentally in Vojvodina. J Serbian Chem Soc 2008; 73:283-297.
25. Montenegro L,  Sarpietro MG,  Ottimo S,  Puglisi G, Castelli F. Differential scanning calorimetry studies on sunscreen loaded solid lipid nanoparticles prepared by the phase inversion temperature method. Int J Pharm 2011; 415:301-306.
26. Qian C, Decker EA, Xiao H, McClements DJ. Solid lipid nanoparticles: Effect of carrier oil and emulsifier type on phase behavior and physical stability. J Am Oil Chem Soc 2012;89:17-28.
27. Pathak P, Nagarsenker M. Formulation and evaluation of lidocaine lipid nanosystems for dermal delivery. AAPS Pharm Sci Tech 2009; 10:985-992.
28. Cerreto F,  Scalzo M,  Cesa S,  Paolicelli P, Casadei M. Solid lipid nanosuspensions based on low melting lipids as protective system of retinyl palmitate. J Drug Deliv Sci Technol 2011; 21:479-483.
29. Fluhr J, Lademann J. This Issue at a Glance and an Invitation to the Forum following the Gordon Research Conference. Skin Pharmacol Physiol 2007; 20:121-121.
30. Zhang J, Purdon CH, Smith EW. Solid lipid nanoparticles for topical drug delivery. Am J Drug Deliv 2006; 4:215-220.
31. Muller RH, Petersen RD, Hommoss A, Pardeike J. Nanostructured lipid carriers (NLC) in cosmetic dermal products. Adv Drug Deliv Rev 2007; 59:522-530.
32. Wissing S, Muller R. The influence of the crystallinity of lipid nanoparticles on their occlusive properties. Int J Pharm 2002; 242:377-379.