Comparison of acute effects of heroin and Kerack on sensory and motor activity of honey bees (Apis mellifera)

Document Type : Original Article

Author

Department of Biology, School of Sciences, Shahed University, Tehran, Iran

Abstract

Objective(s):Previous studies demonstrated a functional similarity between vertebrate and honey bee nervous systems. The aim of the present study was to compare the effects of heroin and Iranian street Kerack, a combination of heroin and caffeine, on sensory threshold and locomotor activity in honey bees.
Materials and Methods: All drugs were given orally to honey bees 30 min before each experiment. The levels of these drugs and their metabolites in brain samples of honey bees were determined by GC/MS. The sucrose sensitivity test was used for evaluation of changes in honey bees’ sensory threshold. Following the administration of both drugs, the honey bees’ locomotor activity changes were evaluated in open fields.
Results: 6-acetylmorphine had a higher concentration in comparison with other heroin metabolites in honey bees’ brains. Concentration of the compound in the brain was directly proportional to the amount ingested. Heroin reduced the sensory threshold of honey bees, but Kerack increased it in the same doses. Locomotor activity of honey bee in open field was enhanced after the administration of both drugs. However, immobility time of honey bees was only affected by high doses of heroin.
Conclusion: Acute effects of heroin andKerack on the sensory and motor functions of honey bees were different. Findings of this research suggest that these differences originated from the activation of different neurotransmitter systems by caffeine together with activation of opioid receptors by heroin.

Keywords


1. Pouletty P. Drug addictions: towards socially accepted and medically treatable diseases. Nat Rev Drug Discov 2002; 1:731-736.
2. Morrison J, Thornton V, Ranaldi R. Chronic intermittent heroin produces locomotor sensitization and long-lasting enhancement of conditioned reinforcement. Pharmacol Biochem Behav 2011; 99:475-479.
3. Blum J, Gerber H, Gerhard U, Schmid O, Petitjean S, Riecher-Rössler A, et al. Acute effects of heroin on emotions in heroin-dependent patients. Am J Addict 2013; 22:598-604.
4. Mysels DJ, Sullivan MA. The relationship between opioid and sugar intake: review of evidence and clinical applications. J Opioid Manag 2010; 6:445-452.
5. Alam Mehrjerdi Z. Crystal in Iran: methamphe-tamine or heroin Kerack. Daru 2013; 21:22.
6. Klous MG, Nuijen B, Van den Brink W, Van Ree JM, Beijnen JH. Development and manufacture of diacetylmorphine/caffeine sachets for inhalation by ‘chasing the dragon’ by heroin addicts. Drug Dev Ind Pharm 2004; 30:775–784.
7. Klous MG, Nuijen B, Van den Brink W, Van Ree JM, Beijnen JH. Process characterisation, optimisation and validation of production of diacetylmorphine/caffeine sachets: a design of experiments approach. Int J Pharm 2004; 285:65–75.
8. Klous MG1, Lee W, Hillebrand MJ, van den Brink W, van Ree JM, Beijnen JH. Analysis of diacetylmorphine, caffeine, and degradation products after volatilization of pharmaceutical heroin for inhalation. J Anal Toxicol 2006; 30: 6-13.
9. Griffiths P, Gossop M, Powis B, Strang J. Transitions in patterns of heroin administration: a study of heroin chasers and heroin injectors. Addiction 1994; 89:301-309.
10. Rush CR, Baker RW, Wright K. Acute behavioral effects and abuse potential of trazodone, zolpidem and triazolam in humans. Psychopharmacol 1999; 144:220-233.
11. Kuribara H, Uchihashi Y. Caffeine enhances acute stimulant effect of morphine but inhibits morphine sensitization when assessed by ambulation of mice. Prog Neuropsychopharmacol Biol Psychi 1995; 19:313-321.
12. Mustard JA, Edgar EA, Mazade RE, Wu C, Lillvis JL, Wright GA. Acute ethanol ingestion impairs appetitive olfactory learning and odor discrimination in the honey bee. Neurobiol Learn Mem 2008; 90:633-643.
13. Chittka L, Niven J. Are bigger brains better? Curr Biol 2009; 19:R995-R1008.
14. Fu Y, Chen Y, Yao T, Li P, Ma Y, Wang J. Effects of morphine on associative memory and locomotor activity in the honey bee (Apis mellifera). Neurosci Bull 2013; 29:270-278.
15. Ellen CW, Mercer AR. Modulatory actions of dopamine and serotonin on insect antennal lobe neurons: insights from studies in vitro. J Mol Histol 2012; 43:401-404.
16. Topper SM, Aguilar SC, Topper VY, Elbel E, Pierce-Shimomura JT. Alcohol disinhibition of behaviors in C. elegans. PLoS One 2014; 9:e92965.
17. Barron AB, Maleszka J, Vander Meer RK, Robinson GE Maleszka R. Comparing injection, feeding and topical application methods for treatment of honey bees with octopamine. J Insect Physiol 2007; 53:187-194.
18. Mousavi SM, Imani S, Haghighi S, Mousavi SE, Karimi A. Effect of Iranian honey bee (Apis mellifera) venom on blood glucose and insulin in diabetic rats. J Arthropod Borne Dis 2012; 6:136-143.
19. Farhoudian A, Sadeghi M, Khoddami Vishteh HR, Moazen B, Fekri M, Rahimi Movaghar A. Component analysis of Iranian Kerack; a newly abused narcotic substance in Iran. Iran J Pharm Res 2014; 13:337-344.
20. Djozan D, Baheri T. Preparation and evaluation of solid-phase microextraction fibers based on monolithic molecularly imprinted polymers for selective extraction of diacetylmorphine and analogous compounds. J Chromatogr A 2007; 1166:16-23.
21. Huang ZY, Robinson GE, Tobe SS, Yagi KJ, Strambi C, Strambi A, Stay B. Hormonal regulation of behavioural development in the honey bee is based on changes in the rate of juvenile hormone biosynthesis. J Insect Physiol 1991; 37:733-741.
22. Mahdy T, El-Shihi TH, Emara MM, Chericoni S, Giusiani M, Giorgi M. Development and validation of a new GC-MS method for the detection of tramadol, O-desmethyltramadol, 6-acetylmorphine and morphine in blood, brain, liver and kidney of Wistar rats treated with the combination of heroin and tramadol. J Anal Toxicol 2012; 36:548-559.
23. Matsuda K, Nakatoh S, Minatogawa Y, Suzuki K, Kimura H, Yamamoto S, et al. Development of GC/MS library for analyzing pesticides and drugs. Rinsho Byori 2003; 51:963-968.
24. Bitterman ME, Menzel R, Fietz A, Schaefer S. Classical conditioning of proboscis extension in honey bees (Apis mellifera). J Comp Physiol A 1983; 97:107-119.
25. Haupt SS, Klemt W. Habituation and dishabituation of exploratory and appetitive responses in the honey bee (Apis mellifera L.). Behav Brain Res 2005; 165: 12–17.
26. Aliouane Y, EL Hassani AK, Gary V, Armengaud C, Lambin M, Gauthier M. Subchronic exposure of honey bees to sublethal doses of pesticides: effects on behavior. Environmental Tofcol Chem 2009; 28:113-122.
27. Huber R, Panksepp JB, Nathaniel T, Alcaro A, Panksepp J. Drug-sensitive reward in crayfish: an invertebrate model system for the study of seeking, reward, addiction, and withdrawal. Neurosci Biobehav Rev 2011; 35:1847-1853.
28. Moufid-Bellancourt S, Velley L. Effects of morphine injection into the parabrachial area on saccharin preference: modulation by lateral hypothalamic neurons. Pharmacol Biochem Behav 1994; 48:127-133.
29. Bertino M, Beauchamp GK, Engelman K. Naltrexone, an opioid blocker, alters taste perception and nutrient intake in humans. Am J Physiol 1991; 261:R59-R63.
30. Martinović-Mitrović S, Dickov A, Mitrović D, Dickov V. Reaction time in relation to duration of heroin abuse. Srp Arh Celok Lek 2011; 139:69-75.
31. Xi ZX, Stein EA. Increased mesolimbic GABA concentration blocks heroin self-administration in the rat. J Pharmacol Exp Ther 2000; 294:613-619.
32. El Hassani AK, Dacher M, Gary V, Lambin M, Gauthier M, Armengaud C. Effects of sublethal doses of acetamiprid and thiamethoxam on the behavior of the honey bee (Apis mellifera). Arch Environ Contam Toxicol 2008; 54:653–661.
33. Kay LM, Stopfer M. Information processing in the olfactory systems of insects and vertebrates. Sem Cell Dev Biol 2006; 17:433–442.
34. Kucharski R, Maleszka R. Microarray and real-time PCR analyses of gene expression in the honey bee brain following caffeine treatment. J Mol Neurosci 2005; 27:269-276.
35. Crickmore MA, Vosshall LB. Opposing dopaminergic and GABAergic neurons control the duration and persistence of copulation in Drosophila. Cell. 20137; 155:881-893.
36. Ribeiro JA, Sebastião AM. Caffeine and adenosine. J Alzheimers Dis. 2010; 20 Suppl 1:S3-15.
37. Adler EM. Tasting the bitter and the sweet, honey bee memories, and visualizing calcium throughout entire astrocytes. J Gen Physiol 2013; 141: 511-512.
38. Fredholm BB, Battig K, Holmen J, Nehlig A, Zvartau EE. Actions of caffeine in the brain with special reference to factors that contribute to its widespread use. Pharmacol Rev 1999; 51:83-133.
39. Mustard JA1, Dews L, Brugato A, Dey K, Wright GA. Consumption of an acute dose of caffeine reduces acquisition but not memory in the honey bee. Behav Brain Res 2012; 232:217-224.
40. Andersen JM, Ripel A, Boix F, Normann PT, Mørland J. Increased locomotor activity induced by heroin in mice: pharmacokinetic demonstration of heroin acting as a prodrug for the mediator 6-monoacetylmorphine in vivo. J Pharmacol Exp Ther 2009; 331:153-161.
41. Castellano C, Filibeck L, Oliverio A. Effects of heroin, alone or in combination with other drugs, on the locomotor activity in two inbred strains of mice. Psychopharmacol 1976; 49:29-31.
42. Gottås A, Boix F, Øiestad EL, Vindenes V, Mørland J. Role of 6-monoacetylmorphine in the acute release of striatal dopamine induced by intravenous heroin. Int J Neuropsychopharmacol 2014; 17:1357-1365.
43. Perry CJ, Barron AB. Neural mechanisms of reward in insects. Annu Rev Entomol 2013; 58:543-562.
44. Harano KI, Sasaki M, Nagao T, Sasaki K. Dopamine influences locomotor activity in honey bee queens: implications for a behavioural change after mating. Physiolo Entomol 2008; 33: 395–399.
45. Scheiner R, Baumann A, Blenau W. Aminergic Control and Modulation of Honey bee Behaviour. Curr Neuropharmacol 2006; 4:259–276.
46. Mustard JA. The buzz on caffeine in invertebrates: effects on behavior and molecular mechanisms. Cell Mol Life Sci 2014; 71:1375-382.