Nephroprotective potential of Graptophyllum pictum against renal injury induced by gentamicin


1 Department of Chemistry, Shri Madhwa Vadiraja Institute of Technology and Management, Udupi- 574115, India

2 Department of Pharmaceutical Chemistry, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India

3 Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576104, Karnataka, India


Objective(s):To evaluate the effect of Graptophyllum pictum on lipid peroxidation and tissue antioxidant enzymes in liver and kidney of gentamicin induced nephrotoxic rats.
Materials and Methods: Animals were grouped into 6: Group 1 received gum acacia, Group 2 received G. pictum ethanol extract (300 mg/kg), Group 3 received gentamicin, Groups 4, 5, 6 received gentamicin along with G. pictum at 300, 150, 75 mg/kg, respectively. Nephroprotective activity was evaluated by measuring thiobarbituric acid-reactive substances (TBARS), biochemical markers  Glutathione (GSH), Glutathione-S Transferase(GST), Superoxide dismutase (SOD), Catalase (CAT), serum urea and creatinine levels.
Results: Results obtained showed that gentamicin induced nephrotoxic rats exhibited lower activities of biochemical markers and raised levels of TBARS, serum creatinine and urea. Remarkably, after treatment with G. pictum extract, anomalous levels of biochemical markers, lipid peroxidation and serum creatinine were returned to normal.
Conclusion: The results propose that G. pictum has nephroprotective effects, and can be a promising natural source against gentamicin induced nephrotoxicity.


1. Silan C, Uzun O, Comunoglu NU, Gokcen S, Bedirhan S, Cengiz M. Gentamicin-induced nephrotoxicity in rats ameliorated and healing effects of resveratrol. Biol Pharm Bull 2007; 30:79-83.
2. Tavafi M, Hasan A, Toolabi P. Inhibitory effect of olive leaf extract on gentamicin-induced nephrotoxicity in rats. Iran J Kidney Dis 2012; 6:25-32.
3. Kim YH, Kim YW, Oh YJ, Back NI, Chung SA, Chung HG, et al. Protective effect of the ethanolic extract of the roots of Brassica rapa on cisplatin induce nephrotoxicity in LLC-PK1 cells and rats. Biol Pharm Bull 2006; 29:2436-2441.
4. Kasahara YS, Mangunkawatjia S. Medicinal Herb index in Indonesia. Jakarta: P.T. Eisai, Indonesia; 1986. p. 318.
5. Olagbende-Dada SO, Ogbonnia SO, Coker HAB, Upko GE. Blood glucose lowering effect of aqueous extract of Graptophyllum pictum (Linn) Griff. on alloxan-induced diabetic rats and its acute toxicity in mice. Afr J Biotechnol 2010; 10:1039-1043.
6. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95:351-358.
7. Sedlak J, Lindsay RH. Estimation of total protein-band and nonprotein sulfhydryl group in tissue with Ellman’s reagent. Anal Biochem 1968; 25:192-205.
8. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 1971; 44:276-287.
9. Aebi H. Catalase in vitro. Methods Enzymol 1984; 105:121-126.
10. Ghalia AA, Rabboh NA,  Shalakani A, Seada L, Khalifa A. Estimation of glutathione S-transferase and its Pi isoenzyme in tumor tissues and sera of patients with ovarian cancer. Anticancer Res 2000; 20:1229-1235.
11.  Soliman KM, Abdul-Hamid M, Othman AI. Effect of carnosine on gentamicin-induced nephrotoxicity. Med Sci Monit 2007; 13:73-83.
12. Lopez-Novoa JM, Quiros Y, Vicente L, Morales AI, Lopez-Hernandez FJ. New insights into the mechanism of aminoglycoside nephrotoxicity: an integrative point of view. Kidney Int 2011; 79:33-45.
13. Kalayarasan S, Prabhu PN, Sriram N, Manikandan R, Arumugam M, Sudhandiran G. Diallyl sulphide enhances antioxidants and inhibits inflammation through the activation of Nrf2 against gentamicin induced nephrotoxicity in Wistar rats. Eur J Pharmacol 2009; 606:162-171.
14. Van de Water B, Zoeteweij JP, Nagelkerke JF. Alkylation-induced oxidative cell injury of renal proximal tubular cells: involvement of glutathione redox-cycle inhibition. Arch Biochem Biophys 1996; 327:71-80.
15. Gilbert DN, Wood CA, Kohlhepp SJ, Kohnen PW, Houghton DC, Finkbeiner HC, et al. Polyaspartic acid prevents experimental aminoglycoside nephrotoxicity. J Infect Dis 1989; 159:945-953.