Antidiabetic effect of honey feeding in noise induced hyperglycemic rat: involvement of oxidative stress

Document Type : Original Article


1 Deptartment of Biology, Basic Sciences Faculty, Sciences and Researches, Azad University, Tehran, Iran

2 Deptartment of Physiology, Physiology Research Center, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran

3 Deptartment of Anatomy, Physiology Research Center, School of Medicine, Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran


Objective(s):In this study the effect of oral administration of honey on serum glucose, lipids, stress oxidative markers, and morphology of langerhans islets in noise induced hyperglycemic rats was investigated.
Materials and Methods: Male Wistar rats were divided into control, hyperglycemic, honey treated control, and honey treated hyperglycemic groups. For induction of hyperglycemia, noise stress was used. Serum glucose, triglyceride (TG), total cholesterol, low density lipoprotein (LDL), and high density lipoprotein (HDL)-cholesterol levels were determined before the study and at 4th and 8th weeks after the study. Markers of oxidative stress in brain were also measured. Morphology of langerhans islets in four groups was evaluated using Gomori staining method.
Results: Treatment of noise induced hyperglycemic rats with honey produced a hypoglycemic effect and appropriate changes regarding serum lipids in treated diabetic group at 4th and 8th weeks as compared to the control group. Meanwhile, honey treatment significantly ameliorated the increased malondialdehyde (MDA) content and reduced the activity of superoxide dismutase (SOD) in brain. Histology of langerhans islets in hyperglycemic group showed a lower number and granularity of beta cells; honey treatment produced beneficial change in this respect.
Conclusion: Oral administration of honey in experimental model of diabetes showed a significant hypoglycemic effect and led to appropriate changes in serum lipid profiles.


1. Mahmood R, Khan GJ, Alam S, Safi AJ. Effect of 90 decibel noise of 4000 hertz on blood pressure in young adults. J Ayub Med Coll Abbottabad 2003; 16:30-33.
2. Babisch W. The noise/stress concept, risk assessment and research needs. Noise  Health 2002; 4:1.
3. Babisch W. Epidemiological studies of the cardiovascular effects of occupational noise-a critical appraisal. Noise Health 1998; 1:24.
4. Theebe MA. Planes, trains, and automobiles: the impact of traffic noise on house prices. J Real Estate Finance and Economics 2004; 28:209-234.
5. Van Kempen EE, Kruize H, Boshuizen HC, Ameling CB, Staatsen BA, de Hollander AE. The association between noise exposure and blood pressure and ischemic heart disease: a meta-analysis. Environ Health Perspect 2002; 110:307.
6. Maschke C, Harder J, Ising H, Hecht K, Thierfelder W. Stress hormone changes in persons exposed to simulated night noise Noise Health 2002; 5:35.
7. Gate L, Paul J, Ba GN, Tew K, Tapiero H. Oxidative stress induced in pathologies: the role of antioxidants. Biomed Pharmacol 1999; 53:169-180.
8. Packer L, Tritschler HJ, Wessel K. Neuroprotection by the metabolic antioxidant [alpha]-lipoic acid. Free Radic Biol Med 1997; 22:359-378.
9. Fridovich I. Superoxide anion radical (O• ̄2), superoxide dismutases, and related matters. J Biol Chem 1997; 272:18515-18517.
10. Madrigal JL, Olivenza R, Moro MA, Lizasoain I, Lorenzo P, Rodrigo J, et al. Glutathione depletion, lipid peroxidation and mitochondrial dysfunction are induced by chronic stress in rat brain. Neuropsychopharm 2001; 24:420-429.
11. Van Campen LE, Murphy WJ, Franks JR, Mathias PI, Toraason MA. Oxidative DNA damage is associated with intense noise exposure in the rat. Hear Res 2002; 164:29-38.
12. Molan PC. The antibacterial activity of honey: 2. Variation in the potency of the antibacterial activity. 1992.
13. Molan P. Honey as an antimicrobial agent.  Bee Products: Springer; 1997.p.27-37.
14. Beretta G, Granata P, Ferrero M, Orioli M,                  Maffei Facino R. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Anal Chim Acta 2005; 533:185-191.
15. Nagai T, Inoue R, Kanamori N, Suzuki N, Nagashima T. Characterization of honey from different floral sources. Its functional properties and effects of honey species on storage of meat. Food Chem 2006; 97:256-262.
16. Martos I, Ferreres F, Yao L, D'Arcy B, Caffin N, Tomás-Barberán FA. Flavonoids in monospecific Eucalyptus honeys from Australia. J Agric  Food Chem 2000; 48:4744-4748.
17. Machha A, Mustafa MR. Chronic treatment with flavonoids prevents endothelial dysfunction in spontaneously hypertensive rat aorta. J Cardiovasc Pharmacol 2005; 46:36-40.
18. Estevinho L, Pereira AP, Moreira L, Dias LG, Pereira E. Antioxidant and antimicrobial effects of phenolic compounds extracts of Northeast Portugal honey. Food Chem Tox 2008; 46:3774-3779.
19. Roghani M, Baluchnejadmojarad T. Chronic epigallocatechin-gallate improves aortic reactivity of diabetic rats: underlying mechanisms. Vascul Pharmacol 2009; 51:84-89.
20. Roghani M, Baluchnejadmojarad T. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology 2010; 17:55-59.
21. Roghani M, Baluchnejadmojarad T. Mechanisms underlying vascular effect of chronic resveratrol in streptozotocin‐diabetic rats. Phytother Res 2010; 24:S148-S54.
22. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18:499-502.
23. Carrasco GA, Van de Kar LD. Neuroendocrine pharmacology of stress. Euro J Pharm 2003; 463:235-272.
24. Makino S, Asaba K, Nishiyama M, Hashimoto K. Decreased type 2 corticotropin-releasing hormone receptor mRNA expression in the ventromedial hypothalamus during repeated immobilization stress. Neuroendocrinology 1999; 70:160-167.
25. la Fleur SE, Akana SF, Manalo SL, Dallman MF. Interaction between corticosterone and insulin in obesity: regulation of lard intake and fat stores. Endocrinology 2004; 145:2174-2185.
26. Gao B, Kikuchi-Utsumi K, Ohinata H, Hashimoto M, Kuroshima A. Repeated immobilization stress increases uncoupling protein 1 expression and activity in Wistar rats. Jpn J Physiol 2003; 53:205-214.
27. Akana SF, Strack AM, Hanson ES, Horsley CJ, Mulligan ED, Bhatnagar S, et al. Interactions among chronic cold, corticosterone and puberty on energy intake and deposition. Stress 1999; 3:131-46.
28. Lin YH, Liu AH, Xu Y, Tie L, Yu HM, Li XJ. Effect of chronic unpredictable mild stress on brain–pancreas relative protein in rat brain and pancreas. Behav Brain Res 2005; 165:63-71.
29. Toleikis PM, Godin DV. Alteration of antioxidant status in diabetic rats by chronic exposure to psychological stressors. Pharmacol Biochem  Behav 1995; 52:355-366.
30. Gill-Sharma M, D’Souza S, Parte P, Balasinor N, Choudhuri J, Majramkar D, et al. Effect of oral tamoxifen on semen characteristics and serum hormone profile in male bonnet monkeys. Contraception 2003; 67:409-413.
31. Abdul-Ghani AS, Dabdoub N, Muhammad R, Abdul-Ghani R, Qazzaz M. Effect of palestinian honey on spermatogenesis in rats. J Med Food 2008; 11:799-802.
32. Siti A. Honey and reproductive hormones. Malays J Med Sci 2007; 14:105-106.
33. Kenjerić D, Mandić ML, Primorac L, Bubalo D, Perl
A. Flavonoid profile of< i> Robinia</i> honeys produced in Croatia. Food Chem 2007; 102:683-690.
34. Mirshekar M, Roghani M, Khalili M, Baluchnejadmojarad T, Moazzen SA. Chronic oral pelargonidin alleviates streptozotocin-induced diabetic neuropathic hyperalgesia in rat: involvement of oxidative stress. Iran Biomed J 2010; 14:33.
35. Yanardağ R, Bolkent Ş, Özsoy‐Saçan Ö, Karabulut‐Bulan Ö. The effects of chard (Beta vulgaris L. var. cicla) extract on the kidney tissue, serum urea and creatinine levels of diabetic rats. Phytother Res 2002; 16:758-761.