Plasma Nitric Oxide and Acute Phase Proteins after Moderate and Prolonged Exercises

Document Type : Original Article


1 Department of Chemical Pathology and Immunology, Faculty of Basic Medical Sciences, Olabisi Onabanjo University, Ago-iwoye, Nigeria

2 Department of Chemical Pathology and Immunology, University College Hospital, Ibadan, Nigeria


The aim of this study was to evaluate plasma levels of nitric oxide (NO) and certain acute phase proteins
(caeruloplasmin, transferrin, haptoglobin, C-reactive protein) in Nigerian subjects after short and prolonged exercises.
Materials and Methods
A total of 57 subjects (34 males and 23 females) between ages of 19 and 45 years participated in this study and were
divided into three groups: Group 1 (Prolonged exercise) consisted of footballers (10 males and 9 females) who played
football for at least two hrs daily; Group II (Moderate exercise) consisted of individuals (14 males and 5 females) who
played football for 30 min 3 times a week; Group III (10 males and 9 females) were sedentary workers, who rarely had
any form of physical exercise. The plasma samples were assayed for NO, C-reactive protein (CRP), caeruloplasmin,
haptoglobin, and transferrin using spectrophotometer and immunoplates. Statistical analysis was done using the
The result showed that there was a significant reduction in the level of NO in prolonged exercise (P<0.05) when
compared with control subjects while the increase of NO in subjects with moderate exercise was not statistically
significant when compared with control subjects. C-reactive protein was significantly increased (P< 0.01) while
transferrin and haptoglobin were significantly reduced (P< 0.001 and P< 0.01 respectively) in subjects with prolonged
exercise when compared with control subjects. In moderate exercise, haptoglobin was significantly reduced (P< 0.05)
while the reduction in the levels of caeruloplasmin and transferrin was not statistically significant when compared with
control subjects. The mean level of CRP was significantly raised in prolonged exercise compared with controls or
moderate exercise while the level of caeruloplasmin was significantly reduced in prolonged exercise compared with
controls or moderate exercise.
Moderate exercises should be encouraged.


1. Stampfer M, Hu F, Manson J, Rimm E, Willett W. Primary prevention of coronary heart disease in women through diet and lifestyle. N Eng J Med 2000; 343:16-23.
2. Wenger NK, Froelicher ES, Smith LK, Ades PA, Berra K, Blumenthal JA, et al. Cardiac Rehabilitation as Secondary Preventio; Clinical Practice Guideline No. 17. Rockville, Md: US Department of Health and Human Services, Public Health Service, Agency for Health Care Policy and Research and the National Heart, Lung, and Blood Institute;. ACHCPR Publication No. 1995.p. 96-0672.
3. Spirduso WW. Physical fitness, aging, and psychomotor speed: a review. J Gerontol 1985; 35:850-865.
4. Martinsen EW, Medhus A, Sandvik L. Effects of aerobic exercise on depression: a controlled study. Br Med J Clin Res Ed 1985; 291:109-116.
5. Nieman DC. Exercise and resistance to infection. Canada J Physiol Pharmacol 1998; 76: 573-580.
6. Arner P, Kriegholm E, Engfeldt P, Bolinder J. Adrenergic regulation of lipolysis in situ at rest and during exercise. J Clin Invest 1990; 85:893–898.
7. Adedapo KS, Akinosun OM, Odegbemi BO, Arinola OG, Adedeji OI. Plasma biochemical changes during moderate and vigorous exercises. Int J Sport Sci Eng. 2009; 3:73-76.
8. Green DJ, Maiorana A, O'Driscoll G, Taylor R. Effect of exercise training on endothelium-derived nitric oxide function in humans. J Physiol 2004; 561:1-25.
9. Jungersten L, Anneli A, Björn W, Åke W. Both physical fitness and acute exercise regulate nitric oxide formation in healthy humans. J Appl Physiol 1997; 82:760-764.
10. Bode-Boger S, Boger R, Schroder E, Frolich J. Exercise increases systemic nitric oxide production in men. J Cardiovasc Risk 1994; 1:173-178.
11. Gilligan DM, Panza JA, Kilcoyne CM, Waclawiw MA, Casino PR, Quyyumi AA. Contribution of endothelium-derived nitric oxide to exercise-induced vasodilation. Circulation 1994; 90:2853-2858.
12. Morley JJ, Kushner I. Serum C-reactive protein levels in disease. Ann N Y Acad Sci 1982; 389: 406-418.
13. Gabay C, Kushner I. Acute-phase proteins and other systemic responses to inflammation. N Engl J Med 1999; 340:1376.
14. Maes M, Delange J, Ranjan R. Acute phase proteins in schizophrenia, mania and major depression: modulation by psychotropic drugs. Psychiatry Res 1997; 66:1-11
15. Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnosk JS, Tannenbaum SR. Analysis of Nitrate, Nitrite and (N-15N)-labelled nitrate in biological fluids. Ann Biochem 1982; 126:131-138.
16. Mackinnon LT. Advances in Exercise Immunology. Champaign, IL: Human Kinetics Books;1999.p.149–151.
17. Laudanska E, Gwuzdz A, Brudel G, Rajchet J. Evaluation of Griess reagent and TTC tests in obstetrics. Ginekol Pol 1970; 41:857-862.
18. Arinola OG, Arowojolu A, Bamgboye A, Akinwale A, Adeniyi FAA.Serum concentrations of immunoglobulins and acute phase proteins in Nigerian women with preeclampsia. Repr. Biol. 2006; 6: 265-274.
19. Kobashigawa JA, Leaf DA, Gleeson MP. Benefit of cardiac rehabilitation in heart transplant patients: a randomized trial. J Heart Lung Transplant.1994; 90: S77.
20. Ades PA, Waldmann ML, Gillespie C. A controlled trial of exercise training in older coronary patients. J Gerontol Biol Sci Med Sci.1995; 50A: M7-M11
21. Strachan AF, Noakes TD, Kotzenberg G, Nel AE, de Beer FC. C reactive protein concentrations during long distance running. Br Med J Clin Res 1984; 289:1249-1251.
22. Pearson TA, Mensah GA, Alexander RW, Anderson JL, Cannon RO III, Criqui M, et al. Markers of inflammation and cardiovascular disease: application to clinical and public health practice: a statement for healthcare professionals from the Centers for Disease Control and Prevention and the American Heart Association. Circulation 2003; 107:499–511.
23. Ridker PM, Rifai N, Rose L, Buring JE, Cook NR. Comparison of C-reactive protein and low-density lipoprotein cholesterol levels in the prediction of first cardiovascular events. N Engl J Med 2002; 347:1557–1565.
24. LaManca JJ, Haymes EM. Effects of iron repletion on VO2max, endurance, and blood lactate in women. Med Sci Sports Exerc 1993; 25: 1386–1392.
25. Puntum FW. Haptoglobin. In: The plasma proteins: Structure, function, and genetic control. 2nd Ed. (Puntum FW eds.), New York: Academic Press; 1975.
26. Hanzawa K, Orihara K, Kubo K, Hiraga A, Watanade S. Changes of two-dimensional electrophoretic patterns of plasma with maximum exercises in young thoroughbred horses. Asian-Aus. J Anim Sci 2000; 13:152-155.