Effect of Vitamin Supplementation on Serum Oxidized Low-Density Lipoprotein Levels in Male Subjects with Cardiovascular Disease Risk Factors

Document Type: Original Article


1 Faculty of Health, Bushehr University of Medical Sciences. Bushehr, Iran

2 Department of Nutrition and Dietetics. Faculty of Medicine and Health Sciences, University Putra Malaysia, Malaysia

3 Isfahan Cardiovascular Research Center, Isfahan University of Medical Sciences. Isfahan, Iran


Oxidized low-density lipoproteins (ox-LDLs) appear to play a significant role in atherogenesis. In fact, circulating ox-LDL concentrations have been recognized as a risk factor for cardiovascular disease (CVD). The main objectives of this study were to assess the effects of antioxidant vitamins on ox-LDL as a biomarker of CVD in male subjects with CVD risk factors.
Materials and Methods
The effect of antioxidant vitamins on ox-LDL as a biomarker of CVD in male subjects with CVD in male subjects with CVD risk factors at baseline and after 12 weeks of supplementation with vitamin E (400 IU), C (500 mg), ß-carotene (15 mg), and the combined supplements (E, C, and ß-carotene) respectively defined as group E, C, B and control group was considered as group P.
The mean values for ox-LDL at the baseline were 86.93 ± 26.30 U/l in group C, 94.52 ± 38.40 U/l in group E, 79.73±2.07 U/l in group B, 85.97±23.07 U/l in combined group, and 84.90± 14.66 U/l in group P. After 12 weeks of intervention the percentage of changes for group C, group E, group B, COM group, and group P were (-18.32), (-2286), (-17.31), (-19.01) and (-2.0), respectively. Using Wilcoxon method, significant differences were detected in the mean ox-LDL concentrations of baseline and after intervention, values in the C, E, B and combined groups (P< 005).
This study illustrated that diet supplemented with vitamin C (500 mg), vitamin E (400 IU), ß-carotene (15 mg), and the combination of these vitamins was associated with lower serum ox-LDL levels.


1. Bjelakovic G, Nikolova D, Gluud LL, Simonetti RG, Gluud C. Mortality in randomized trials of antioxidant supplements for primary and secondary prevention: systematic review and metaanalysis. JAMA 2007; 297:842–857.
2. Abudu N, Miller JJ, Attaelmannan M, Levinson SS.Vitamins in human arteriosclerosis with emphasis on vitamin C and vitamin E. Clinica ChemicaActa2004;339:11–25.
3. Lapointe A,  Couillard C, Lemieux S. Effects of dietary factors on oxidation of low-density lipoprotein particles. J Nutr Biochem2006; 17:645-658.
4. Witztum JL,Steinberg D. The oxidative modification hypothesis of atherosclerosis: does it hold for humans? Trends Cardiovasc Med2001; 11:93–102.
5. Asmis R, Begley JG.Oxidized LDL promotes peroxide-mediated mitochondrial dysfunction and cell death in human macrophages. Circ Res 2003;92:20-27.
6. Koenig W, Khuseyinova N.Biomarkers of atherosclerotic plaque instability and rupture. Arterioscler Thromb Vasc Biol2007; 27:15.
7. Holvoet P, Kritchevsky  SB, Tracy  RB, Mertens  A. The metabolic syndrome, circulating oxidized LDL, and risk of myocardial infarction in well-functioning elderly people in the health, aging, and body composition cohort. Diabetes 2004; 53-59.
8. Weinbrenner T, Cladellas  M, Isabel Covas M. High oxidative stress in patients with stable coronary heart disease. Atherosclerosis 2003; 168:99–106.
9. Pryor WA, Stahl W, Rock CL. Beta-carotene: from biochemistry to clinical trials. Nutr Rev 2000; 58:39-53.
10.  Keaney JF. Oxidative stress and the vascular wall: NADPH oxidases take center stage. Circulation2005; 112:2585–2858.
11. Frei B, Forte TM, Ames BN, Cross CE. Gas phase oxidants of cigarette smoke induce lipid peroxidation changes in lipoprotein properties in human blood plasma. Protective effects of ascorbic acid.Biochem J 1991; 277:133–138.
12. Azzi  A, Gysin  R, Kempna  P, Ricciarelli  R, Villacorta  L, Visarius  T, et al. The role of alpha-tocopherol in preventing disease: from epidemiology to molecular events. Mol Aspects Med 2003; 24:325–336.
13. Omenn GS, Goodman GE, Thornquist MD, Balmes J, Cullen MR, Glass A. Risk factors for lung cancer and for intervention effects in CARET, the beta-carotene and retinol efficacy trial. J Natl Cancer Inst 1996; 88:1550–1559.
14.  Reaven  PD, Khouw  A, Beltz  WF, Parthasarathy  S, Witztum JL. Effect of dietary antioxidant combinations in humans: protection of LDL by vitamin E, but not by beta-carotene. Arterioscler Thromb 1993; 13:590-600.
15. Jialal I, Fuller CJ, Huet  BA. The effect of alpha-tocopherol supplementation on LDL oxidation: A dose-response study. Arterioscler Thromb 1995; 15:190-196.
16. Hodis  HN, Mack WJ, LaBree  L, Mahrer  PR,  Sevabian  A, Liu C, et al. Alpha-tocopherol supplementation in healthy individuals reduces low-density oxidation but not atherosclerosis. Circulation2002; 106:1453–1459.
17. Princen  HMG, Poppel GV, Vogelezang C, Buytenhek R, Kok  F. Supplementation with Vitamin E but not beta-carotene in vivo protects low density lipoprotein from lipid peroxidation in vitro: effect of cigarette smoking. Arteriosc Thromb1992; 12:554-562.
18. Fuller CJ, Grundy SM, Nurkos  EP, Jialal  I. Effect of ascorbate supplementation on low density lipoprotein oxidation in smokers. Arthrosclerosis 1996; 119:139-150.