Study of Radioprotective Effect of Green Tea against Gamma Irradiation Using Micronucleus Assay on Binucleated Human Lymphocytes

Document Type : Original Article

Authors

1 Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Iran

2 Department of Physics, Faculty of Sciences, Ferdowsi University of Mashhad, Iran

3 Cancer Research Centre (CRC), Department of Radiation Oncology, Omid Hospital, Mashhad University of Medical Science, Mashhad, Iran

Abstract

Objective(s)
The aim of this study was to investigate the radioprotective effect of green tea against genotoxicity induced by gamma irradiation in cultured blood lymphocytes from 5 human volunteers.
Materials and Methods
Peripheral blood samples were collected from volunteers before and 1, 3 and 5 hr after drinking a decoction 4 g green tea in 280 ml boiling water for 5 constitutive days with the same quantity. At each time point, the whole blood samples were exposed to 200 cGy of 60 Co gamma irradiation and then were cultured with mitogenic stimulation to determine the chromosomal aberration in micronucleus assay on cytokinesis-blocked binucleated cells.
Results
As expected, for each volunteer, the results showed a significant increase in the incidence of micronuclei after exposure to gamma irradiation as compared to non-irradiated control samples. Only lymphocytes blood sample collected 3 hr after drinking green tea exhibited a significant decrease in incidence of micronuclei compared to non-treated irradiated samples.
Conclusion
These results suggest the radioprotective ability of green tea against ionizing radiation in human lymphocytes, at specified time after consumptior.

Keywords

References

1. Goodhead DT. Initial events in the cellular effects of ionizing radiations: clustered damage in DNA. Int J Radiat Biol 1994; 65:7-17.
2. Iyer R, Lehnert BE. Effects of ionizing radiation in targeted and nontargeted cells. Arch Biochem Biophys 2000; 376:14-25.
3. Reily PA. Free radicals in biology: oxidative stress and the effect of ionizing radiation. Int J Radiat Biol 1994; 65:27–33.
4. Hosseinimehr SJ. Trends in the development of radioprotective agents. Drug Discov Today 2007; 12:794–805.
5. Kouvaris JR, Koulouias VE,Vlahos LJ. Amifostine: The First selective-yrtget and broad-spectrum radioprotector. Oncologist 2007; 12:738-747.
6. Van der Vijgh WJ, Peters GJ. Protection of normal tissues from the cytotoxic effects of chemotherapy and radiation by amifostine (Ethyol): preclinical aspects. Semin Oncol 1994; 21:2-7.
7. Maurya DK, Devasagayam TP, Nair CK. Some novel approaches for radioprotection and the beneficial effect of natural products. Indian J Exp Biol 2006; 44:93-114.
8. Hosseinimehr SJ, Mahmoudzadeh A, Ahmadi A, Mohamadifar S, Akhlaghpoor Sh. Radioprotective effects of hesperidin against genotoxicity induced by gamma-irradiation in human lymphocytes. Mutagenesis 2008; 24:233–235.
9. Hosseinimehr SJ, Mahmoudzadeh A, Azadbakht M, Akhlagpoor Sh. Radioprotective effects of hawthorn against genotoxicity induced by gamma-irradiation in human blood lymphocytes. Radiat Environm Biophys 2009; 48:95–98.
10. Shimoi K, Masuda S, Furugori M, Esaki S, Kinae N. Radioprotective effects of antioxidative flavonoids in γ-ray irradiated mice. Carcinogen 1994; 15:2669-2672.
11. Shimoi K, Masuda S, Furugori M, Esaki S, Kinae N. Radioprotective effects of antioxidative plant flavonoids in mice. Mutat Res 1996; 350:153-161.
12. Tiwari AK. Imbalance in antioxidant defense and human diseases: multiple approach of natural antioxidants therapy. Current Sciences? 2001; 81:1179–1187.
13. Hertog MGL, Hollman PCH, Vanderputte B. Content of potentially anticarcinogenic flavonoids of tea infusions, wines and friut juices. J Agric Food Chem 1993; 41:1242-1246.
14. Cabrera C, Artacho R, Gime´nez R. Beneficial effects of green tea-a review. J Am Coll Nutr 2006; 25:79–99.
15. Fenech M, Morley AA. Measurement of micronuclei in lymphocytes. Mutat Res 1985; 147:29-36.
16. Fenech M. The in vitro micronucleus technique. Mutat Res 2000; 455:81–95.
17. Durakovic CA. Radioprotective agents in medicine. Arh Hig Rada Toksilokol 1993; 44:331-354.
18. Butt MS, Sultan MT. Green Tea: nature’s defense against malignancies. Critl Rev Food Sci Nutr 2009; 49:463–473.
19. Velayutham P, Babu A, Liu D. Green Tea catechins and cardiovascular health: an update. Curr Med Chem 2008; 15:1840–1850.
20. Wang Y, Ho CT. Polyphenolicc Chemistry of tea and coffee: a century of progress. J Agric Food Chem 2009; 57:8109–8114.
21. Elbling L, Herbacek I, Weiss RM, Jantschitsch C, Micksche M, Gerner C, et al. Hydrogen peroxide mediates EGCG-induced antioxidant protection in human keratinocytes. Free Radic Biol Med 2010; 15:49:1444-1452.
22. Li Q, Zhao H, Zhao M, Zhang Z, Li Y. Chronic green tea catechins administration prevents oxidative stress-related brain aging in C57BL/6J mice. Brain Res 2010; 24:1353:28-35.
23. Itoh T, Imano M, Nishida S, Tsubaki M, Hashimoto S, Ito A, Satou T. (-)-Epigallocatechin-3-gallate protects against neural cell death and improves cerebral function after traumatic brain injury in rats. Neuromolecular Med 2011; 13:300-309.
24. Yoo KM, Hwang IK, Moon B. Comparative flavonoids contents of selected herbs and associations of their radical scavenging activity with antiproliferative actions in V79-4 cells. J Food Sci 2009; 74:419-425.
25. Alcaraz M, Armero D, Martínez-Beneyto Y, Castillo J, Benavente-García O, Fernandez H, et al. Chemical genoprotection: reducing biological damage to as low as reasonably achievable levels. Dentomaxillofac Radiol 2011; 40:310-314.
26. Hu Y, Guo D, Liu P, Cao J, Wang Y, Yin J, et al. Bioactive components from the tea polyphenols influence on endogenous antioxidant defense system and modulate inflammatory cytokines after total-body irradiation in mice. Phytomedicine 2011; 18:970-975.
27. Stalmach A, Troufflard S, Serafini M, Crozier A. Absorption, metabolism and excretion of choladi green tea flavan-3-ols by humans. Mol Nutr Food Res 2009; 53:S44 –S53.
28. Stalmach A, Mullen W, Steiling H, Williamson G, Lean MEJ, Crozier A. Absorption, metabolism, and excretion of green tea flavan-3-ols in humans with an ileostomy. Mol Nutr Food Res 2010; 54:323–334.
Iranian Journal of Basic Medical Sciences
Volume 15, Issue 5 - Serial Number 5
September 2012
Pages 1026-1031

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