Effect of Matricaria aurea (Loefl.) Shultz-Bip. Hydroalcoholic Extract on Acetic Acid-Induced Acute Colitis in Rats

Document Type : Original Article


1 Department of Pharmacology and Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

3 Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran


Matricaria aurea is found abundant in Iran and has large similarities in constituents especially essential oils, flavones and flavonoides as well as traditional uses to the main species; Matricaria recutita L. Anti-inflammatory, antioxidant and spasmolytic properties of the main species suggest that this plant may have beneficial effects on inflammatory bowel diseases so the present study was carried out.
Materials and Methods
Hydroalcoholic extract of plant with doses of 200, 400, 800 mg/kg were administered orally (p.o.) for 5 days and rectally (i.r.) (400 and 800 mg/kg) at 15 and 2 hr before ulcer induction. To induce colitis, 2 ml of acetic acid 4% was instilled intra-colonically to separate groups of male Wistar rats (n= 6). Normal saline (2 ml), prednisolone (4 mg/kg) and hydrocortisone acetate (20 mg/kg) enema were administered to control and reference groups respectively. The tissue injures were assessed macroscopically and histopathologically.
Greater doses of extract (400 and 800 mg/kg) reduced colon weight/length ratio (P< 0.01) and the highest test dose (800 mg/kg p.o. or i.r.) was effective to decrease tissue damage parameters including ulcer severity, area and index (P< 0.01) as well as inflammation severity and extent, crypt damage and total colitis index (P< 0.01) significantly.
It is concluded that Matricaria aurea extract was effective to protect against acute colitis in acetic acid model and this effect was more significant with the greater doses administered orally or rectally. Further studies are warranted to ascertain the mechanisms that are involved and the responsible active constituents.


1. McKay DL, Blumberg JB. A review of bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytother Res 2006; 20:519-530.
2. Ghassemi-Dehkordi N. Matricaria. In: Iranian Herbal Pharmacopoeia Scientific Committee, editors, Iranian Herbal Pharmacopeia. 1st ed. Tehran: Iran Ministry of Health & Medical Education Publications; 2002. p. 99- 107.
3. Ahmed AA, Abou Elela MA. Highly oxygenated bisabolenes and an acetylene from Matricaria aurea. Phytochemistry 1999; 51:551-554.
4. Ali-Shtayeh M, Yaniv Z, Mahajna J. Ethnobotanical survey in the Palestinian area. A classification of the healing potential of medicinal plants. J Ethnopharmacol 2000; 73: 221-232.
5. Capasso F, Grandolini G. Fitofarmacia. 2nd ed. Milan: Springer Veralg; 1999.
6. Al-Mustafa AH, Al-Thonibat OY. Antioxidant activity of some Jordanian medicinal plants used traditionally for treatment of diabetes. Pak J Biol Sci 2008; 11:351-358.
7. Sartor RB. Pathogenesis and immune mechanism of chronic inflammatory bowel diseases. Am J Gastroenterol 1997; 92:5S-11S.
8. Brzezinski A. Inflammatory bowel diseases. In: Andreoli TE, Carpenter CCJ,Griggs RC, Loscalzo TE, editors. Cecil essentials of medicine. 5th ed. Philadelphia:Saunders WB Company; 2001.
9. Lih-Brody L, Powell SR, Collier KP, Reddy GM, Cerchia R, Kahn E, et al. Increased oxidative stress and decreased antioxidant defences in mucosa of inflammatory bowel disease. Dig Dis Sci 1996; 41:2078-2086.
10. Bouma C, Strober W. The immunological and genetic basis of inflammatory bowel disease. Nat Rev Immunol 2003; 3:521-533.
11. Mascolo N, Izzo A, Autore G, Maiello F, Dicarlo G, Capsso F. Acetic acid-induced colitis in normal and essential fatty acid deficient rats. J Pharmacol Exp Ther 1995; 272:469- 475.
12. Morris GP, Beck PL, Herridge MS, Depew WT, Szewczuck MR, Wallace JL. Hapten induced model of inflammation and ulceration in rat colon. Gastroenterology 1989; 96:795-803.
13.Minaiyan M, Ghannadi AR, Karimzadeh A.Antiulcerogenic effects of ginger (Zingiber officinale Roscoe) on cysteamine induced duodenal ulcer in rats. DARU 2006; 14:97-101.
14. Vogel HG, Vogel WH. Drug discovery and evaluation (Pharmacological Assays). 2nd ed. Berlin: Springer Verlag;1997.
15. Minaiyan M, Ghannadi A, Mahzouni P, Nabi-Meibodi. Anti-ulcerogenic effect of ginger (rhizome of Zingiber officinale Roscoe) hydroalcoholic extract on acetic acid- induced acute colitis in rats. Res Pharm Sci 2008; 3:15-22.
16. Cooper HS, Murthy SNS, Shah RS, Sedergran DJ. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest 1993; 69:238-249.
17. Dieleman LA, Palmen MJHJ, Akol H, Bloemena E, Pena AS, Meuwissen SGM. Chronic experimental colitis induced by dextran sulfate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol 1998; 114:385-391.
18. MacPherson BR, Pfeiffer CJ. Experimental production of diffuse colitis in rats. Digestion 1978; 17:135-150.
19. Mahgoub AA, El-Medany AH, Hagar HH, Sabah DM. Protective effect of natural honey against acetic acidinduced colitis in rats. Trop Gastroenterol 2002; 23:82-87.
20. Paiva LA, Gurgel LA, De Sousa ET, Silveria ER, Silva RM, Santos FA, et al. Protective effect of Copaifera langsdorffii oleo-resin against acetic acid-induced colitis in rats. J Ethnopharmacol 2004; 93:51-56.
21. Evans WC. Trease and evance pharmacognosy. 15th ed. Edinburgh: Saunders WB; 2002.
22. Khayyal MT, El-Ghazaly MA, Kenawy SA, Seif-El-Nasr M, Mahran LG, Kafafi YA, et al. Antiulcerogenic effect of some gasterointestinally acting plant extracts and their combinations. Arzneimittelforschung 2001; 51:545-553.
23. Gholami A, Aboulhasani Z, Mohagheghzadeh A. Treatment of gastric ulcer by herbal medicine. In: Ebadi M, editor. Pharmacodynamic basis of herbal medicine. 1st ed.Tehran: Rahe Kamal Press Inc; 2007. p. 933-943.
24. Tan PV, Njimi CK, Ayafor JF. Screening of some African medicinal plants for antiulcerogenic activity: part 1. Phytother Res 1997; 11:45-47.
25. Borelli F, Izzo AA. The plant kingdom as a source of antiulcer remedies. Phytother Res 2000; 14:581-591.
26. El-Abhar HS, Hammad LN, Gawad HS. Modulating effects of ginger extract on rats with ulcerative colitis. J Ethnopharmacol 2008; 118:367-372.
27. Jagtap AG, Shirke SS, Phadke AS. Effect of polyherbal formulation on experimental models of inflammatory bowel diseases. J Ethnopharmacol 2004; 90:195-204.