Effects of Chronic Oral Administration of Natural Honey on Ischemia/Reperfusion-induced Arrhythmias in Isolated Rat Heart

Document Type : Original Article


1 Department of Pharmacology, School of Pharmacy and Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran

2 School of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

3 Department of Pathology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

4 School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran


In this study, effects of chronic administration of oral natural honey against ischemia/reperfusion (I/R)-  induced cardiac arrhythmias were investigated in isolated rat heart.
Materials and Methods
Male Wistar rats were divided into four groups (n= 10-14 rats in each group) and fed with natural honey (1%, 2% and 4% dissolved in the drinking water) for 45 days except for the control group. After anesthesia, the rats’ hearts were isolated quickly, mounted on a Langendorff apparatus and perfused with a modified Krebs-Henseleit solution during stabilization, 30 min regional ischemia followed by 30 min reperfusion. The ECGs were recorded throughout the experiments to analyze cardiac arrhythmias based on the Lambeth conventions.
In the ischemic phase, honey (1%) significantly reduced (P<0.05) the number and duration of ventricular tachycardia (VT). Honey (1% and 2%) also significantly decreased number of ventricular ectopic beats (VEBs). In addition, incidence and duration of reversible ventricular fibrillation (Rev VF) were lowered by honey 2% (P<0.05). During reperfusion time, VT incidence was 73% in the control group, however natural honey (1%) decreased it to 22% (P<0.05). Honey also produced significant reduction in the incidences of total VF, Rev VF, duration and number of VT.
For the first time, the results of present study demonstrated protective effects of chronic oral honey administration against I/R-induced arrhythmias in isolated rat heart. Antioxidant activity, the existence of energy sources such as glucose and fructose and improvement of some hemodynamic functions might be responsible for these effects.


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