The Effects of Achillea wilhelmsii Extract on Rat’s Gastric Motility at Basal and Vagal Stimulated Conditions

Document Type : Original Article


1 Department of Physiology and Cardiovascular Research Center, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Biology, Sciences School, Azad University of Mashhad, Mashhad, Iran


Achillea genius is widely used in traditional medicine for gastrointestinal disorders. The aim of this study was to investigate the effects of aqueous-ethanol extract of Achillea wilhelmsii on rat’s gastric motility in basal and vagal stimulated conditions.
Materials and Methods
Twenty four Wistar rats were randomly divided into two groups: control and test. The extract was prepared by maceration which was used to prepare three 0.5 ml samples of three doses (0.5, 1 and 2 mg/kg) in the test group. The same volume of saline was used in the control group. Gastric motility was measured by inserting a small balloon in the stomach which was connected to a pressure transducer. The data were recorded for 25 min duration after each dose and these data were analyzed for 3 intermittent five min intervals (t1= 0-5, t2= 10-15 and t3= 20-25 min).
The extract at basal condition decreased intragastric pressure (IGP) by 1 mg/kg dose in the t3 and 2 mg/kg in the t2 and t3 intervals. The extract at vagal stimulated condition decreased IGP by 1 and 2 mg/kg doses in the t2 and t3 intervals. The extract reduced contraction amplitude at basal condition by 2 mg/kg dose in the t2 and t3 intervals. At vagal stimulated condition contraction amplitude was reduced by 1 mg/kg dose in the t2 and t3 by 2 mg/kg in all three intervals. The extract showed no effect on frequency of gastric contraction in either basal or vagal stimulated conditions.
The extract showed an inhibitory effect on gastric motility in both basal and vagal stimulated condition. This inhibitory effect may be exerted by an antagonistic effect on acetylcholine dependent calcium influx or release of calcium from intracellular storage in gastric smooth muscle.


1. Kasper DL. Harrison's principels of internal medicine. 16th ed. New york:McGraw- Hill medical publishing division; 2005.
2. World Health Organization. Traditional medicine growing needs and potential. Geneva: World Health Organization; 2002.No.2.
3. Saeidnia S, Yassa N, Rezaeipoor R, Shafiee A, Gohari AR, Kamalinejad M, et al. Immunosuppressive principles from Achillea talagonica, an endemic species of Iran. Daru 2009; 17:37-41.
4. Benedek B, Kopp B, Melzig MF. Achillea millefolium L. s.l. is the anti-inflammatory activity mediated by protease inhibition? J Ethnopharmacol 2007; 113:312-317.
5. Candan F, Unlu M, Tepe B, Daferera D, Polissiou M, Sokmen A, Akpulat HA. Antioxidant and antimicrobial activity of the essential oil and methanol extracts of Achillea millefolium subsp. millefolium Afan. (Asteraceae). J Ethnopharmacol 2003; 87:215-220.
6. Stojanovic G, Radulovic N, Hashimoto T, Palic R. In vitro antimicrobial activity of extracts of four Achillea species: the composition of Achillea clavennae L. (Asteraceae) extract. J Ethnopharmacol 2005; 101:185-190.
7. Asgary S, Naderi GH, Sarrafzadegan N, Mohammadifard N, Mostafavi S, Vakili R. Antihypertensive and antihyperlipidemic effects of Achillea wilhelmsii. Drugs Exp Clin Res 2000; 26:89-93.
8. Tozyo T, Yoshimura Y, Sakurai K, Uchida N, Takeda Y, Nakai H, et al. Antitumor sesquiterpenoids in Achillea millefolium. Chem Pharm Bull (Tokyo) 1994; 42:1096-100.
9. Csupor-Löffler B, Hajdú Z, Zupkó I, Réthy B, Falkay G, Forgo P, et al. Antiproliferative effect of flavonoids and sesquiterpenoids from Achillea millefolium s.l. on cultured human tumour cell lines. Phytother Res 2009; 23:672-676.
10. Nemeth E, Bernath J. Biological activities of yarrow species (Achillea spp.). Curr Pharm Des 2008; 14:3151-3167.
11. Lemmens-Gruber R, Marchart E, Rawnduzi P, Engel N, Benedek B, Kopp B. Investigation of the spasmolytic activity of the flavonoid fraction of Achillea millefolium s.l. on isolated guinea-pig ilea. Arzneimittelforschung 2006; 56:582-588.
12. Yaeesh S, Jamal Q ,Khan AU, Gilani AH. Studies on hepatoprotective, antispasmodic and calcium antagonist activities of the aqueous-methanol extract of Achillea millefolium. Phytother Res 2006; 20:546-551.
13. Karamenderes C, Apaydin S. Antispasmodic effect of Achillea nobilis L. subsp. sipylea (O. Schwarz) Bässler on the rat isolated duodenum. J Ethnopharmacol 2003; 84:175-179.
14. Benedek B, Geisz N, Jager W, Thalhammer T, Kopp B. Choleretic effects of yarrow (Achillea millefolium s.l.) in the isolated perfused rat liver. Phytomedicine 2006; 13:702-706.
15. Cavalcanti AM, Baggio CH, Freitas CS, Rieck L, de Sousa RS, Da Silva-Santos JE, et al. Safety and antiulcer efficacy studies of Achillea millefolium L. after chronic treatment in Wistar rats. J Ethnopharmacol 2006; 107:277-2784.
16. Mahady GB, Pendland SL, Stoia A, Hamill FA, Fabricant D, Dietz BM, et al. In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders. Phytother Res 2005; 19:988-9891.
17. Dokhani S, Cottrell T, Khajeddin J, Mazza G. Analysis of aroma and phenolic components of selected Achillea species. Plant Foods Hum Nutr 2005; 60:55-62.
18. Afsharypuor S, Asgary S, Lockwood GB. Constituents of the essential oil of Achillea wilhelmsii from Iran. Planta Med 1996; 62:87-78.
19. Gherase F, Spac A, Dorneanu V, Stănescu U, Grigorescu E. Pharmacognostic research of some species of Achillea. Note 1. Volatile oils analysis. Rev Med Chir Soc Med Nat Iasi 2003; 107:188-191. (Article in Romanian)
20. Javidian K, Miri R, Sadeghpour H. Compsition of the volatile oil of Achillea wilhelmsii C. Koch from Iran. Daru 2004; 12:63-66.
21. Nabavizadeh Rafsanjani F, Vahedian J. The effect of insulin-dependent diabetes mellitus on basal and distention-induced gastric acid and pepsin secretion in rat. Diabetes Res Clin Pract 2004; 66:1-6.
22. Niazmand S, Hosaini KH, Derakhshan M. The effects of Ziziphora clinopodioides Lam. extract on rat’s gastric motility at basal and vagal stimulated conditions.Pharmacologyonline (newsletter) 2009; 2:734-740
23. Rotondo A, Serio R, Mulè F. Gastric relaxation induced by apigenin and quercetin: analysis of the mechanism of action. Life Sci 2009; 85:85-90.
24. Wells RW, Lourenssen S, Blennerhassett MG. Impaired acetylcholine-induced smooth muscle contraction in colitis involves altered calcium mobilization and AKT phosphorylation. Pflugers Arch 2008; 456:507-517.
25. Bergner A, Sanderson MJ. Acetylcholine-induced calcium signaling and contraction of airway smooth muscle cells in lung slices. J Gen Physiol 2002; 119:187-198.