Effects of Combined Sonodynamic and Photodynamic Therapies on a Colon Carcinoma Tumor Model

Document Type : Original Article


1 Research Center and Department of Medical Physics, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Medicinal Chemistry, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of pathology, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Community Medicine& Public Health, Mashhad University of Medical Sciences Mashhad, Iran


Although photodynamic therapy is considered as a noninvasive method, most photosensitizers are susceptible to  ultrasound.  Therefore,  it  is  expected  that  the  combination of  two  activation  methods  might  have  a synergistic effect. This probable effect has been investigated in this study.
Materials and Methods
This  study  was  conducted  on  colon  carcinoma  tumor  in  Balb/c  mice.  The  tumors  were  induced  by subcutaneous injection of CT26 cells.   Ultrasound and light irradiations were performed on tumors 24 hr after injection of liposomal Zn (II)-phthalocyanine. The treatment efficacy was evaluated using daily measurement of the tumor dimensions.
Ten days post treatment, relative tumor volumes of all groups were significantly reduced in comparison with the main control group. The best response was observed when one of the two treatment methods had been applied. The longest doubling time of tumor was related to the treatment group namely photodynamic, sonodynamic and combination technique, while the shortest belonged to the control group.
This study showed that liposomal Zn phthalocyanine is both photosensitizer and sonosensitizer. Photodynamic and sonodynamic therapies can be efficient in retarding tumor growth rate. In this study, the combination of two methods didn’t show any improvement in therapeutic outcomes. It is predicted that latest results are related to the treatments sequence and could be optimized in the future.


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