The Differences in the Relaxant Effects of Different Fractions of Rosa damascena on Guinea Pig Tracheal Smooth Muscle

Document Type : Original Article

Authors

1 Pharmacological Research Center of Medicinal Plants, Medical School, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Physiology, Medical School and Pharmaceutical Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s)
In the present study, the differences in the relaxant effects of aqueous, ethyl acetate and n-butanol fractions of Rosa damascena on tracheal smooth muscle of guinea pigs were examined.
Materials and Methods
The relaxant effects of three cumulative concentrations of each fraction (0.1, 0.2 and 0.4 g%) in comparison with saline and three cumulative concentrations of theophylline (0.1, 0.2, and 0.4 mM) were examined on precontracted tracheal chains of guinea pig by 60 mM KCl (group 1, n= 5) and 10 pM methacholine (group 2, n= 8).
Results
In group 1, all concentrations of theophylline, ethyl acetate fraction and two final concentrations of n-butanol fraction showed significant relaxant effects in comparison with saline (p< 0.05 to P< 0.001). In group 2, all concentrations of theophylline, ethyl acetate and aqueous fractions showed concentration dependent relaxant effects compared to that of saline (p< 0.01 to P< 0.001). In addition, the effect of ethyl acetate fraction in group 1 was significantly higher than those of theophylline (p< 0.05 to p< 0.001). However, the effects of other fractions were significantly lower than theophylline in both groups (p< 0.01 to p< 0.001). There were significant correlations between the relaxant effects and concentrations for theophylline and all fractions (except aqueous fractions in group 1) in both groups.
Conclusion
The results showed a potent relaxant effect for ethyl acetate fraction of R. damascena on tracheal smooth muscle comparable to that of theophylline but a relatively weak relaxant effect for aqueous and n-butanol fractions at concentrations used.

Keywords

References

1.Green M. The Rose. Aromatic thymes; 1999; 7:11-15.
2.Buckle J. Clinical aromatherapy in nursing. London:Arnold, copublished by singular;1997.
3.Libster M, Libster M. Delmars integrative herb guide for nurses. Delmar, Thomson Learning; 2002; 360-370.
4.Wood G, Bache F. The dispensatory of the United States of America .4thed. Philadelphia:Griggand Elliot;1839.
5.Ave-Sina. Law in Medicine. Interpreter; Sharafkhandy A.Tehran:Minishy of Guidance publication; 1990.p.129-131.
6.Zargari A. Medicinal plants. 5th ed. Tehran:Tehran University publications;1992.Vol.2.p.281-284.
7.Mahmood N, Piacenet S, Pizza C, Bruke A, Khan A, Hay A. The anti -HIV activity and mechanisms of action of pure compounds isolated from Rosa damascena. Biochem Biophys Res Commun 1996; 229:73-79.
8.Maleev A, Neshtev G, Stoianov S, Sheikov N. The ulcer protective and antiinflamatory effect of Bulgarian Rose oil. Article in Bulgarian. Eksp Med Morfol; 1972; 11:55-60.
9.Hosseini M, Rakhshandah H, Shafieenic R, Dolati K. Analgesic effect of Rosa damascena on mice. Abstract book of 16th Iranian congress of physiology and pharmacology; 2003; 167.
10.Kumar N, Bhandari P, Singh B, Bari SS. Antioxidant activity and ultra-performance LC-electrospray ionization-quadrupole time-of-flight mass spectrometry for phenolics-based fingerprinting of Rose species: Rosa damascena, Rosa bourboniana and Rosa brunonii. Food Chem Toxicol 2009; 47:361-367.
11.Basim E, Basim H. Antibacterial activity of Rosa damascena essential oil. Fitoterapia 2003; 74: 394-396.
12.Ramezani R, Moghimi A, Rakhshandeh H, Ejtehadi H, Kheirabadi M. The effect of Rosa damascena essential  oil on the amygdala electrical kindling seizures in rat. Pak J Biol Sci 2008; 11:746-751.
13.Rakhshandah H, Hosseini M. Potentiation of pentobarbital hypnosis by Rosa damascena in mice. Indian J Exp Biol 2006; 44:910-912.
14.Jafari M, Zarban A, Pham S,Wang T. Rosa damascena decreased mortality in adult Drosophila. J Med Food 2008; 11:9-13.
15.Kuriyama1 H, Watanabe1 S, Nakaya1 T, Shigemori1 I, Kita1 M, Yoshida N, et al. Immunological and psychological benefits of aromatherapy massage. Evid Based Complement Alternat Med 2005; 2:179-184.
16.Shafei MN, Boskabady MH. Antitussive effect of Rosa damascena in guinea pigs. Iran J Pharmac Res; 2003; 2: 231-234.
17.Boskabady MH, Kiani S, Rakhshandeh H. Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol 2006; 106:377-382.
18.Holroyde MC. The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol 1986; 87:501-507.
19.Martin CAE, Naline E, Bakdach H, Advenier C. Beta3 adrenoceptor agonists, BRL 37344 and SR 58611A do not induce relaxation of human, sheep and guinea-pig airway smooth muscle in vitro. Eur Respir J 1994; 7:1610-1615.
20.Linden A, Bergendal A, Ullman A, Skoogh BE, Lofdahl CG. Salmetrol, formetrol, and salbutamol in the isolated guinea-pig trachea: differences in maximum relaxant effect and potency but not in functional antagonism. Thorax 1993; 48:547-553.
21.Popa VT, Somani P, Simon P, Simon V. The effect of inhaled verapamil on resting bronchial tone and airway constriction by histamine and acetylcholine in normal and asthmatic subjects. Am Rev Respir Dis 1984; 130:106¬113.
22.Leonards B, Rampart M, Herman AG. Selective M3 muscarinic receptor inhibit smooth muscle contraction in rabit trachea without increasing the release of acetylcholine. J Pharmacol Exp Ther 1992; 263:773-770.
23.Miyahara Y, Kizawa Y, Sano M, Murakami H. Effect of organic and inorganic Ca2+ antagonists on acetylcholine induced contraction in molluscan (Mytilus edulis) smooth muscle. Gen Pharmacol 1993; 24:1419-1423.
24.McCaig D, DeJonckeere S. Effect of two Ca2+ modulator in normal and albumin sensitized guinea pig trachea. Eur J Pharmacol 1993; 249:53-63.
25.Buckle DR, Arch JRS, Boering NE, Foster KA, Taylor JF, Taylor SG, et al. Relaxation effect of potassium channel activators BRL 38227 and Pinacidil on guinea-pig and human airway smooth muscle, and blockade of their effects by Glibenclamide and BRL 31660. Pulm Pharmacol 1993; 6:77-86.
26.Perez-Guerrero C, Suarez J, Herrera MD, Marhuenda E. Spasmolytic effect of tetrazepam on rat duodenum and guinea pig ileum. Pharmacol Res 1997; 35:493-494.

Files

Share

How to cite

Statistics