Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor

Fatemi, Farnaz; Pakzad, Parviz; Zeraati, Hojjat; Talebi, Saeed; Asgari, Soheila; Akhondi, Mohammad-Mehdi; Chamani-Tabriz, Leili

doi:10.22038/ijbms.2010.5111

Comparative Molecular and Microbiologic Diagnosis of Vaginal Colonization by Group B Streptococcus in Pregnant Women during Labor

Document Type : Original Article

Authors

¹ Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

² Department of Microbiology, Islamic Azad University, North of Tehran Branch, Tehran, Iran

³ Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

⁴ Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

⁵ International Campus, Tehran University of Medical Sciences, Kish, Iran

10.22038/ijbms.2010.5111

Abstract

Objective(s)
Rapid tests for detection of Streptococcus agalactiae or Group B Streptococci (GBS) at the onset of labor are needed to permit early intrapartum antibiotic prophylaxis. This study aimed to evaluate the PCR assays targeting the 16S ribosomal RNA gene (16S rDNA) for detection of the GBS in comparison with a specific culture method.
Materials and Methods
Two swabs were used to obtain vaginal specimens from the 330 pregnant women attended delivery room at Hedayat hospital, Tehran, Iran. One swab was analyzed by direct plating onto selective GBS agar medium (ISLAM) and the other swab was used for a PCR assay, which amplified the 16S rDNA of S. agalactiae. Comparative study between the selective culture and the PCR assay was done among the 330 tested women. Results
The GBS colonization rate based on the culture results was 20.6% (68/330). Both culture and PCR methods were positive for 56 and negative for 253 women. The culture method was positive and PCR was negative in 12 women. The culture was negative and the PCR positive for 9 women. Sensitivity of the PCR assay was 82.3% and specificity was 96.5%. The positive predictive value was 86.15% and negative predictive value was 95.4%.
Conclusion
ISLAM diagnostic procedure and PCR are rapid and reliable analyzing methods, which might be useful for accurate diagnosis of GBS colonization in pregnant women at the time of delivery.

Keywords

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