Comparison of Prooxidant-antioxidant Balance Method with Crocin Method for Determination of Total Prooxidant-antioxidant Capacity

Document Type : Original Article

Authors

1 Department of Biological Chemistry, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece

2 Department of Nutrition and Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

3 Department of Chemistry, Laboratory of Food Chemistry and Technology, Aristotle University of Thessaloniki, Thessaloniki, Greece

Abstract

Objective(s)
Comparison of prooxidant-antioxidant balance (PAB) assay with crocin assay.
Materials and Methods
Twenty eight serum samples were chosen, PAB and the total antioxidant capacity were measured by PAB assay and crocin, respectively, and the correlation of both assays, along with their correlation with other clinical and biochemical parameters, were determined.
Results
A significant negative correlation was established between PAB assay and crocin assay. Also a significant negative correlation was established between PAB and uric acid and creatinine.
Conclusion
The results showed that by increasing the total antioxidant capacity, which is showed by crocin, the PAB shifts in favor of antioxidants, which is showed by PAB assay. Now, it could be considered that the PAB, along with other risk factors, might help in the prediction of the risk for cardiovascular events; and further research could clarify whether by application of PAB assay and appropriate interventions for correcting oxidative stress, progression of the cardiovascular disease could be reduced.

Keywords

References

1.Alamdari DH, Paletas K, Pegiou T, Sarigianni M, Befani C, Koliakos G. A novel assay for the evaluation of the prooxidant-antioxidant balance, before and after antioxidant vitamin administration in type II diabetes patients. Clin Biochem 2007; 40:248-254.
2.Erel O.A new automated colorimetric method for measuring total oxidant status. Clin Biochem 2005; 38:1103-11011.
3.Erel O. A novel automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem 2004;37:112-119.
4.Bors W, Michel C, Saran M. Inhibition of the bleaching of the carotenoid crocin. A rapid test for quantifying antioxidant activity. Biochim Biophys Acta 1984; 796:312-319.
5.Bors W, Michel C, Saran M. Determination of rate constants for antioxidant activity and use of the crocin assay. In: Ong ASH, Packer L, editors. In Lipid Soluble Antioxidants: Biochemistry and Clinical Applications. Birkhauser Verlag: Basel, Switzerland; 1992. p. 53-63.
6.Tubaro F, Micossi E, Ursini F. The antioxidant capacity of complex mixtures by kinetic analysis of crocin bleaching inhibition. J Am Oil Chem Soc 1996; 73:173-179.
7.Tubaro F, Ghiselli A, Rapuzzi P, Maiorino M, Ursini F. Analysis of plasma antioxidant capacity by competition kinetics. Free Radic Biol Med 1998; 24:1228-1234.
8.Ordoudi SA, Tsimidou MZ. Crocin bleaching assay step by step: observations and suggestions for an alternative validated protocol. J Agric Food Chem 2006 ;54:1663-1671.
9.Sugherini L, Valentini M, Cambiaggi C, Tanganelli I, Gragnoli G, Borgogni P, et al. Determination of a redox compensation index and its relationships to glycaemic control in type 2 diabetes mellitus. Clin Chem Lab Med 2000 ;38:983-987.
10.Prior RL, Cao G. In vivo total antioxidant capacity: comparison of different analytical methods. Free Radic Biol Med 1999; 27:1173-1181.
11.Trotti R, Carratelli M, Barbieri M, Micieli G, Bosone D, Rondanelli M, et al. Oxidative stress and a thrombophilic condition in alcoholics without severe liver disease. Haematologica 2001; 86:85-91.
12.Josephy PD, Mason RP, Eling T. Cooxidation of the clinical reagent 3, 5 3'5'-tetramethylbenzidine by prostaglandin synthase. Cancer Res 1982;42:2567-2570. 
13.MisonoY, Ohkata Y, Morikawa T, Itoh K. Resonance Raman and absorption spectroscopic studies on the electrochemical oxidation processes of 3, 3', 5, 5'-tetramethylbenzidine. J Electroanal Chem 1997; 436:203-212.
14.Gutteridge JM, Halliwell B. The measurement and mechanism of lipid peroxidation in biological systems. Trends Biochem Sci 1990 ;15:129-135.
15.Wilson PW, D'Agostino RB, Levy D, Belanger AM, Silbershatz H, Kannel WB. Prediction of coronary heart disease using risk factor categories. Circulation 1998 12; 97:1837-1847.
16.Bierman EL. George Lyman Duff Memorial Lecture. J AHA, Atherogenesis in diabetes. Arterioscler Thromb 1992; 12:647-656.
17.Glass CK, Witztum JL. Atherosclerosis. the road ahead. Cell 2001;104:503-516.
18.Jay D, Hitomi H, Griendling KK. Oxidative stress and diabetic cardiovascular complications. Free Radic Biol Med 2006; 40:183-192.
19.Stocker R, Keaney JF, Jr. Role of oxidative modifications in atherosclerosis. Physiol Rev 2004; 84:1381-478.
20.Madamanchi NR, Hakim ZS, Runge MS. Oxidative stress in atherogenesis and arterial thrombosis: the disconnect between cellular studies and clinical outcomes. J Thromb Haemost 2005;3:254-267.
21.Alamdari DH, Ghayour-Mobarhan M, Tavallaie S, Parizadeh MR, Moohebati M, Ghafoori F. et al.Prooxidant-antioxidant balance as a new risk factor in patients with angiographically defined coronary artery disease. Clin Biochem 2008; 41: 375-380.

Files

Share

How to cite

Statistics