Evaluation of Hair Growth Activity of Buxus wallichiana Baill Extract in Rats

Document Type : Original Article


1 Department of Pharmacognosy, Sree Siddhaganga College of Pharmacy, Tumkur, Karnataka, India

2 Department of Pharmacognosy, Sri K.V.College of Pharmacy, Chickballapur, Karnataka, India

3 Department of Pharmacognosy, PES College of Pharmacy, Bangaluru, Karnataka, India

4 Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India

5 Department of Pharmaceutical Biotechnology, Sri Krishan Chaitanya College of Pharmacy, Madanapalle, Andhra Pradesh, India

6 Department of Pharmacy, Andhra University, Vishakapatanam, Andhra Pradesh, India


The aim of this study was to evaluate antioxidant and hair growth activities of Buxus wallichiana Baill (Buxaceae).
Materials and methods
Petroleum ether, chloroform, methanol and aqueous extracts of Buxus wallichiana subjected to antioxidant activity by; 2, 2-diphenyl-1-picryl hydrazyl and nitric oxide methods. Methanol extract of Buxus wallichiana at 50, 100 mg/kg, ointment of methanol extract at 5 and 10% used for the evaluation of hair growth property.
Methanol extract showed potential antioxidant activity. Methanol extract at 100 mg/kg showed consistent and significant increase in mean score of hair growth from day 3 to day 24. Whereas 50 mg/kg increased the mean score significantly, only from day 15 to day 24. When methanol extract at 10% applied topically, significant increase in mean hair score observed only from day 15, but at 5% showed considerable increase in mean hair score only from day 21 and 24, when compared to the control.
The result of this study suggests that Methanol extract of Buxus wallichiana possess good antioxidant and hair growth activity.


1.Butler H. Poucher’s Perfumes, Cosmetics and Soaps. 9th ed. Chapman and Hall: London; 1993.   Vol.3.p.130.
2.Olsen EA, Weiner MS, Amara IA, Delory ER, Five-year follow-up of men with androgenetic alopcia treated with topical minoxidil. Brit. J Am Acad Dermatol 1990; 22: 643-646.
3.Wilson C, Walkden V, Powell S, Shaw S, Wilkinson J, Dawber R. Contact dermatitis to 2% topical minoxidil solution. Brit. J Am Acad Dermatol 1991; 24: 661-662.
4. Bhalerao SS, Solanki N H. Therapeutic approaches to the management of common baldness. Indian     Drugs 2002; 39: 567.
5.Matsuda H, Yamazaki M, Matsuo K, Assanuma Y, Kubo M. Anti-androgenic activity of Myricae        cortex isolation of active constituents from bark of Myrica rubra. Biol Pharm Bull 2001; 24: 259-63.
6.Matsuda H, Yamazaki M, Naruo S, Asanuma Y, Kubo M. Anti-androgenic and hair growth promoting        activities of Lygodii spora (spore of Lygodium japonicum) I. Active constituents inhibiting testosterone       5 alpha-reductase. Biol Pharm Bull 2002; 25: 622-26.
7. Takahashi T, Kamiya T, Yokoo Y. Procyanidin oligomers selectively and intensively promote       proliferation of mouse hair epithelial cells in vitro and active hair follicle growth in vivo. J Invest Dermatol 1999; 112: 310-16.
8.Takahashi T, Kamiya T, Yokoo Y. Proanthocyanidins promote hair follicle cell proliferation and the   anagen phase of hair growth.  Acta Derm Venereol 1998; 78: 428-432.
9.Takahashi T, Kamimura A, Shirai A, Yokoo Y. Several selective protein kinase C inhibitors including procyanidins promote hair growth.  Skin Pharmacol Appl Skin Physiol 2000; 13:133-142.
10.Sasajima, Μ Hotta Y. Mechanism of hair growth promoting by 3,4’ dimethyl –3 - hydroxy flavanone. European Hair Growth Society. 2003. Available at: http://www.ehrs.org/conferenceabstracts/2003 barcelona/researchposterindex1.htm
11.Proanthocyanidins–Nature’s minoxidil. Available at: www.stophairlossnow.com.
12.Fleming T. PDR for herbal medicines. New Jersey, Montvale: Medical Economic Company; 1999.
13.Chopra RN, Ayer SL, Chopra IC. Glossary of Indian medicinal plants. New Delhi: Council of Scientific and Industrial Research (CSIR); 1992.
14.Kritikar KR, Basu BD. Indian medicinal plants. 2nd ed. Dehradun, Delhi: Bishon Singh Mahendra Pal Singh and Periodical Experts; 1989.
15.Husain Α, Virman OP, Popli SP, Mishra LN, Gupta Μ, Srivastava GN, et al. Dictionary of medicinal plants. Lucknow: Central Institute of Medicinal and Aromatic Plants Publication; 1992.p. 89.
16.Kvaltinova Z, Lukovie L, Machova Μ Fatranska M. Effect of steroidal alkaloid buxaminol E on blood pressure, acetyl cholinesterase activity and (tritiated) quinuclidinyl benzilate binding in cerebral cortex. Pharmacol 1991; 43:20-25.
17.Ata A,Naz S, Choudhary MI, Atta-ur-Rahman, Sener B, Turkoz S. New triterpenoidal alkaloids from Buxus sempervirens. Z Naturforsch 2002; 57:21-28.
18.Atta-ur-Rahman Au, Ata A, Naz S, Choudhary MI, Sener B, Turkoz S. New steroidal alkaloids from the roots of Buxus sempervirens. J Nat Prod 1999; 62: 665-669.
19.Kokate CK. Practical Pharmacognosy. 2nd ed. New Delhi: Vallabh Prakashan; 1986.
20. Organization for Economic Co-operation and Development (OECD) 423 guidelines for the testing of chemicals (acute oral toxicity), 2000; 1-14.
21.Hwang BY, Kim H.S, Lee JH, Hong YS, Ro JS, Lee KS, et al Antioxidant benzolyated flavan-3-ol glycoside from Celastrus orbiculatus. J Nat Prod 2001; 64: 82-84.
22. Garrat DC. The quantitative analysis of drugs. Chapman and Hall, Japan, Vol.3 1964.
23.Marcocci L, Packer L, Droy-Lefaix, MT, Sekaki A, Gardes-Albert M. Antioxidant action of Ginkgo biloba extracts EGB 761. Methods Enzymol 1994; 234:462-475.
24.Saraf S, Pathak AK, Dixit VK. Hair growth promoting activity of Tridax procumbens. Fitoterapia 1991; 62: 495-98.
25.Ishida H, Inaoka Y, Okada M, Fukushima M, Fukazawa H, Tsuji K. Studies of the active substances in herbs used for hair treatment.  III. Isolation of hair-regrowth substances from Polygara senega var. latifolia TORR et GRAY. Biol Pharm Bull 1999; 22: 1249-50.