Relative Electropermeability and Electric Pulse Effectiveness in Human Breast Adenocarcinoma: An in vitro Study

Document Type : Original Article


1 Research Center of Medical Physics, Avicenna Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

2 Zabol University of Medical Sciences, Zabol, Iran

3 Research Center of Cancer; Avicenna Research Institute; Mashhad University of Medical Science, Mashhad, Iran

4 Department of Community Medicine &Public Health, Mashhad University of Medical Sciences, Mashhad, Iran


This study was carried out in order to evaluate the effects of electrochemotherapy, electrical pulses and chemotherapeutic drugs on the killing of cancerous cells and their probable synergistic effects.
Materials and Methods
Electrochemotherapy treatments conducted on MCF-7 cell line derived from human breast adenocarcinoma tumor using four chemotherapeutic drugs including bleomycin, cisplatin, adriamycin and cyclophosphamide and six electrical doses. Cell survival assayed using MTT method, 72 hrs after the treatment; also the killing effects of each drug and electric dose determined. Finally, “Relative Pulse Effectiveness” and “Relative Electropermeability Effectiveness” calculated.  
All electrical doses decreased cell survival, significantly for bleomycin and cisplatin, however, they were only, significant in high concentration of cyclophosphamide and adriamycin. For the applied drugs, “Relative Electropermeability Effectiveness” was more than one (1.00), except for adriamycin.   
It seems that for the diffusion of molecules into cells, application of high duration electric pulses is more efficient for high molecular weight drugs while for low molecular weight drugs, strong pulses are more effective. In intermediate molecular weight, there is no difference between increasing the pulse strength    and/or duration to achieve additional electropermeability. Electropermeability effect of different electric doses and electrochemotherapy efficiency can be evaluated by “REE” and “RPE”, respectively.


1.Miklavcic D, Kotnik T. Electroporation for electrochemotherapy and gene therapy. Ljubljana, Slovenia: University of Ljubljana; 2004.
2.Pucihar G, Mir LM, Miklavcic D. The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy. Bioelectrochemis 2002; 57:167-172.
3.Sersa G, Krzic M, Sentjurc M, Ivanusa T, Beravs K, Kotnik V, et al. Reduced blood flow and oxygenation in SA-I tumors after electrochemotherapy with cisplatin. Br J Cancer 2002; 87:1047-1054.
4.Sazgarnia A, Khoie A, Bahreyni MH, Mahmoudi M, Feyzi R. In vitro and in vivo studies on enhanced effects of electrical pulses in photodynamic therapy with 5ALA. Iran J Medical Physi 2005; 3:37-50.
5.Sersa G, Stabuc B, Cemazar M, Miklavcic D, Rudolf Z. Electrochemotherapy with cisplatin: the systemic antitumour effectiveness of Cisplatin can be potentiated locally by the application of electric pulses in the treatment of malignant melanoma skin metastases. Melanoma Res 2000; 10:381-385.
6.Yanai H, Kubota Y, Nakada T. Effects of electropermeabilization after the administration of anticancer drugs on transitional cell carcinoma. BJU International 2002; 89: 438-442.
7.Kranjc S, Cemazar M, Grosel A, Sentjurc M, Sersa G.Radiosensitising effect of electrochemotherapy with bleomycin in LPB sarcoma cells and tumors in mice. BMC cancer 2005; 5:115.
8.Sersa G, Miklavcic D, Cemazar M, Rudolf Z, Pucihar G, Snoj M. Electrochemotherapy in treatment of tumors. Eur J Surg Oncol 2008; .34: 232-240.
9.Sersa G, Cemazar M, Miklavcic D, Rudolf Z. Electrochemotherapy of tumours. Radiol Oncol 2006; 40:163-174.
10.Besic E. Physical mechanisms and methods employed in drug delivery to tumors. Acta Pharm 2007; 57:249-268.