Determination of SPF and Moisturizing Effects of Liposomal and Conventional Formulations of Octyl Methoxycinnamte as a Sunscreen

Document Type : Original Article

Authors

1 Department of Pharmaceutics, School ofPharmacy and Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Pharmaceutics, School ofPharmacy and Pharmaceutical Research Center, Mashhad University of Medical Sciences

3 Department of Pharmaceutics, School of Pharmacy and Biotechnology Research Center, Mashhad University of Medical Sciences

4 Australian Photobiology Testing Facility, University of Sydney, Sydney, Australia

Abstract

Objective
To determine and compare the SPF (Sun Protection Factor) and moisturizing effects of the liposomal and conventional lotion formulations containing octyl methoxycinnamte (OMC) as a sunscreen by in vivo methods.
Materials and Methods
The multilamellar liposomes (MLVs) containing OMC were prepared by fusion method and o/w emulsion was prepared as FDA standard sunscreen method. The SPFs of the formulations were determined by in vivo method according to Australian standard. The exposure area was the back of ten volunteers. Subsites of the backs were exposed to solar simulator as ultraviolet (UV) source. The minimum erythemal dose (MED) for unprotected skin was observed in the next day. The sunscreen was spread (2 mg/cm2) over the area with a finger stall to achieve a uniform film. Each test subsite in a series was exposed to controlled amounts of simulated sunlight by a constant ratio. In the third day, the MEDs of the formulations were observed. The SPF was determined by the ratio between the time required to produce the minimal erythematous reaction by using sunscreen and the time needed to produce the same reaction without using sunscreen. The moisture content of the skin was determined after 30 min, 2, 3, 6 and 10 hours post-application of the formulations containing OMC and also NaCl 3% in eucerin (as a positive control) using Corneometer by measuring electrical capacitance.
Results
The SPF obtained from our in vivo results for standard Homosalate reference was almost the same as published SPF for this standard. The SPF of the liposomes containing OMC was a little bit more than lotion at the same concentration of OMC. All the tested formulations significantly increased the moisture content of the skin compared to control (without any treatment), in all the tested point times. After 30 minutes of post-application, the skin moisture content resulted from OMC-lotion was significantly more than liposomal-OMC and NaCl 3% in eucerin; however, 10 hours after post-application there were no significant differences in the skin moisture content of these three treatment groups.
Conclusion
MLV liposomes prepared by fusion method is a good vehicle for OMC as a sunscreen since it provides proper SPF and increase the moisture content of the skin.

Keywords

References

1.Kullavanijaya P, Henry W. Photoprotection. J Am Acad Dermatol 2005; 52: 937-958.
2.Wilkinson JB, Moore RJ. Harry’s Cosmeticology. Singapore: Longman Scientific & Technical; 1996. 222¬260.
3.Burke KE. The value of sunscreens. Int J Dermatol 1999; 38: 88-90.
4. Periolia L, Ambrogi V, Bertini B, Ricci M, Nocchetti M, Latterini L, Rossi C. Anionic clays for sunscreen agent safe use: Photoprotection, photostability and prevention of their skin penetration. Eur J Pharm Biopharm 2006; 62: 185-193. 
5.Couteau C, Cullel NP, Connan AE, Coiffard LJM. Stripping method to quantify absorption of two sunscreens in human. Int J Pharm 2001; 222: 153-157.
6.Treffel P, Gabard B. Skin penetration and sun protection factor of ultra-violet filters from two vehicles. Pharm Res 1996; 13: 770-774.
7.Cummings SR, Tripp MK, Hemmann B. Approaches to the prevention and control of skin cancer. Cancer Metastasis Rev 1997; 16: 309-327.
8.Sheu MT, Lin CW, Huang MC, Shen CH, Ho HO. Correlation of in vivo and in vitro measurements of sun protection factor. Int J Cos Sci 2003; 11: 128-132.
9.Foldvari M. Effect of vehicle on topical liposomal drug delivery: petrolatum bases, J Microencapsul 1996; 13: 589-600.
10.Mezei M. Liposomes and the skin. In: Gregoriadis G, Florence AT, Patel HM, (eds). Liposomes in Drug Delivery. Switzerland Harwood Academic Publishers GmbH; 1993, 25-135.
11.Verma DD, Verma S, Blume G, Fahr A. Particle size of liposomes influences dermal delivery of substances into skin. Int J Pharm 2003; 258: 141-151.
12.Foldvari M, Gesztes A, Mezei M. Dermal drug delivery by liposome encapsulation. Clinical and electron microscopic studies. J Microencapsul 1990; 7: 479-482.
13.Mezei M, Gulasekharam V. Liposomes-a selective drug delivery system for the topical route of administration: a gel dosage form. J Pharm Pharmacol 1982; 34: 473-474.
14.Gregoriadis G, Kirby C, Large P, Meehan A, Senior J. Targeting of liposomes: study of influence factors. In: Gregoriadis G, Senior J. (eds). Targeting of Drugs. New York: Plenium Press; 1981, 155-184.
15.Foldvari M, Blair J, Oguejiofor JN. Topical dosage form of liposomal tetracaine: effect of additives on the in vitro release and in vivo efficacy. J Control Release 1993; 27: 193-205.
16.Ramon E, Alonso C, Coderch L, de la Maza A, Lopez O, Parra JL, Notario J. Liposomes as alternative vehicles for sun filter formulations. Drug Deliv 2005; 12: 83-88.
17.Harish M, Patel M, Moghimi M. Liposomes and the skin Permeability barrier. In: Gregoriadis G, Florence AT, Patel HM. (eds). Liposomes in Drug Delivery. Switzerland: Harwood Academic Publishers GmbH. 1993, 137-147.
18.Suzuki K, Sakon K. The application of liposomes to cosmetics. Cosmetic & Toiletries 1990; 105: 65-78.
19.Bernacchi F, Puglia C, Citernesi U, Bonina F. Efficacy evaluation of sun products formulated with radical- scavenger in liposome complexes. Ricerche Technologie Cosmeticologiche 2000; 1-15.
20.Barenholz Y, Crommelin JA. Liposomes as pharmaceutical dosage forms. In: Swarrick J, Boylan JC. (eds). Encyclopedia of Pharmaceutical Technology. New York: Marcel Dekker Inc.1994, 1-33.
21.Food and Drug Administration. U.S. Department of Health and Human Services, 1999. Sunscreen drug products for over the counter human use: final monograph. Federal Register May 21, 64(98): 27666-27693.
22.Foldvari M. 1998, Biphasic Liposomes. In US Patent. No. 5,853,755.
23.New RRC. Liposomes: a Practical Approach. New York: Oxford University Press.1990, 105-160.
24.Chisvert A, Pascual-Marti MC, Salvador A. Determination of UV-filters in sunscreens by HPLC. Fresenius J Anal Chem 2001; 369: 638-641.
25.Jimenez MM, Pelletier J, Bobin MF, Martini MC. Influence of encapsulation on the in vitro percutaneous absorption of octyl methoxycinnamate. Int J Pharm 2004; 272: 45-55.
26.Fang JY, Hwang TL, Huang YL, Fang CL. Enhancement of the transdermal delivery of catechins by liposomes incorporating anionic surfactants and ethanol. Int J Pharm 2006; 310: 131-138.
27.Australian/New Zealand Standard. Sunscreen products. Evaluation and classification. AS/NZS 2604:1998, 1-32.
28.Matts PJ. Solar ultraviolet radiation: definitions and terminology. Dermatol Clin 2006; 24: 1-8.
29.Sato PG, Schmidt JB. Honigsmann H. Comparison of epidermal hydration and skin surface lipids in healthy individuals and in patients with atopic dermatitis. J Am Acad Dermatol 2003; 352-358.
30.Alanen E, Nuutinen J, Niclen K, Lahtinen T, Monkkonen J. Measurement of hydration in the stratum corneum with the MoistureMeter and comparison with the Corneometer. Skin Res Technol 2004; 10: 32-37.
31.Dykes P J. What are meters measuring? Int J Cos Sci 2002; 24: 241-245.
32.Gustavsson H, Farbrot A, Larko O. Percutaneous absorption of benzophenone-3, a common component of topical sunscreens. Clin Exp Dermatol 2002; 27: 691-694.
33.Springsteen A, Yurek R, Frazier M, Carr F. In vitro measurement of sun protection factor of sunscreens by diffuse transmittance. Anal Chim Acta 1999; 380: 155-164. 
34.Chatelain E, Gabard B, Surber C. Skin penetration and sun protection factor of five UV filters: Effect of the vehicle. Skin. Pharmacol. Appl Skin Physiol 2003; 16: 28-35.
35.Fernandez C, Marti-Mestres G, Ramos J, Maillols H. LC analysis of benzophenone-3: II application to determination of “in vitro” and “in vivo” skin penetration from solvents, coarse and submicron emulsions. J Pharm Biomed Anal 2002; 24: 155-165.
36.Sood A, Venugopalan P, Venkatesan N, Vyas SP. Liposomes in cosmetics and skin care. Indian Drugs 1995; 33: 43-49.
37.Ghyczy M, Gareiss J. Liposomes from vegetable phosphatidylcholine. Cosmetic & Toiletries 1994; 109: 75-81.
38.Skalko N, Cajkovab M, Jalsenjak I. Liposomes with clindamycin hydrochloride in the therapy of Acne vulgaris. Int J Pharm 1991; 85: 97-101.
39.Shaku M, Kuroda H, Okura A. Enhancing stratum corneum functions. Cosmetic & Toiletries 1997; 112: 65-76.
40.Straianse SJ. Human skin-moisturizing mechanism and natural moisturizers. Cosmetics & Toiletries 1978; 93: 37-41.
41.Oleniacz WS. 1976, In U.S. Patent 3, 957,971.
42.Sone T, Hanamizu T, Ichioka M, Yokokura T. Moisturizing effect of vesicles formed from monoglycerides on human skin. Int J Cos Sci 1999; 21: 23-31.
43.Idson B, Lazarus J. Semisolids. In: Lachman L, Lieberman HA, Kanig JL. (eds). The Theory and Practice of Industrial Pharmacy. Philadelphia: Lea & Febiger. 1986, 534-563.
44.Fluhr JW, Gloor M, Lazzerini S, Kleesz P, Grieshaber R, Berardesca E. Comparative study of five instruments measuring stratum corneum hydration (Corneometer CM 820 and CM 825, Skicon 200, Nova DPM 9003, Dermalab). Part I. In vitro. Skin Res Technol 1999; 5: 161-170.
45.Rawlings AV, Canestrari DA, Dobkoweski B. Moisturizer technology versus clinical performance. Dermato Ther 2004; 17: 49-56.

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